Based on the field studies conducted in August 2005 and 2007, the distributions of zooplankton structure and abundance, as well as chlorophyll content, in the Sheksna Reservoir are analyzed. A total of 107 zooplankton species have been found (46 Cladocera, 18 Copepoda, and 43 Rotifera), including 24 species (10 Cladocera, 2 Copepoda, and 12 Rotifera) new for the reservoir. In the surface layer of water, where blue green algae concentrate, zooplankton abundance and biomass are 1.5-5 times lower and the zooplankton structure is simpler than in deeper layers. The highest zooplankton biomass (>3 g/m 3 ) are observed in Lake Beloe and the upper area of the Sheksna section, while in the near shore sites it did not exceed 1 g/m 3 . There are no significant changes in zooplankton abundance when compared to the abundance recorded at the end of the past century.