Comparative- and network-based proteomic analysis of bacterial chondronecrosis with osteomyelitis lesions in broiler’s proximal tibiae identifies new molecular signatures of lameness

Abstract

Bacterial Chondronecrosis with Osteomyelitis (BCO) is a specific cause of lameness in commercial fast-growing broiler (meat-type) chickens and represents significant economic, health, and wellbeing burdens. However, the molecular mechanisms underlying the pathogenesis remain poorly understood. This study represents the first comprehensive characterization of the proximal tibia proteome from healthy and BCO chickens. Among a total of 547 proteins identified, 222 were differentially expressed (DE) with 158 up- and 64 down-regulated proteins in tibia of BCO vs. normal chickens. Biological function analysis using Ingenuity Pathways showed that the DE proteins were associated with a variety of diseases including cell death, organismal injury, skeletal and muscular disorder, immunological and inflammatory diseases. Canonical pathway and protein–protein interaction network analysis indicated that these DE proteins were involved in stress response, unfolded protein response, ribosomal protein dysfunction, and actin cytoskeleton signaling. Further, we identified proteins involved in bone resorption (osteoclast-stimulating factor 1, OSFT1) and bone structural integrity (collagen alpha-2 (I) chain, COL2A1), as potential key proteins involved in bone attrition. These results provide new insights by identifying key protein candidates involved in BCO and will have significant impact in understanding BCO pathogenesis.

Effect of heat stress on the hypothalamic expression profile of water homeostasis‐associated genes in low‐ and high‐water efficient chicken lines

“…The reverse transcription reaction was performed at 42°C for 30 min followed by an incubation at 85°C for 5 min. Real‐time quantitative PCR (Applied Biosystems 7500 Real‐Time PCR system) was performed using 5 μL of 10X diluted cDNA, 0.5 μM of each forward and reverse specific primer, and PowerUp SYBR Green Master Mix (ThermoFisher Scientific, Rockford, IL) in a total 20 μL reaction as previously described (Cook et al., 2023 ; Dridi et al., 2012 ). Oligonucleotide primers specific for chicken aquaporins (AQP1, 2, 3, 4, 5, 7, 8, 9, 10, 11, and 12), arginine vasopressin (AVP) and its related receptors (AVPR1A, AVPR1B, and AVPR2), natriuretic peptide A (NPPA), renin‐angiotensin‐aldosterone (RAAS) system (renin [REN], angiotensinogen [AGT], angiotensin I‐converting enzyme [ACE], angiotensin II receptor type 1 and 2 [AT1 and AT2]), calbindin 1 and 2 (CALB1 and 2), secretin (SCT), and transient receptor potential cation channel subfamily V member 1 and 4 (TRPV1 and TRPV4), and ribosomal 18S as a housekeeping gene are compiled in Table 1 .…”