Comparative and temporal transcriptome analysis of peste des petits ruminants virus infected goat peripheral blood mononuclear cells

Manjunath, Siddappa; Mishra, Bishnu Prasad; Mishra, Bina; Sahoo, Aditya Prasad; Tiwari, Ashok Kumar; Rajak, Kaushal Kishor; Muthuchelvan, D.; Saxena, Shikha; Santra, Lakshman; Sahu, Anamika; Wani, Sajad A.; Singh, R. P.; Singh, Yash Pal; Pandey, Awadh Bihari; Kanchan, Sonam; Kumar, Girish; Janga, Sarath Chandra

doi:10.1016/j.virusres.2016.12.014

Cited by 30 publications

(46 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, severe outbreaks of PPR in regions having large sheep populations have also been reported (Singh et al, 2004a; Raghavendra et al, 2008; Maganga et al, 2013). Recently, host–virus interaction studies in PPR have uncovered transcription factors modulating immune response to Sungri/96 live attenuated vaccine strain and predicted an immune signaling pathway that induces immune response (Manjunath et al, 2015, 2017). However, the host miRNAome in PPR has not been explored till date.…”

Section: Introductionmentioning

confidence: 99%

Modulation of Host miRNAs Transcriptome in Lung and Spleen of Peste des Petits Ruminants Virus Infected Sheep and Goats

et al. 2017

Self Cite

View full text Add to dashboard Cite

Peste des petits ruminants (PPR) is one of the highly contagious viral disease, characterized by fever, sore mouth, conjunctivitis, gastroenteritis, and pneumonia, primarily affecting sheep and goats. Reports suggested variable host response in goats and sheep and this host response vis-a-vis the expression of microRNAs (miRNAs) has not been investigated. Here, miRNAs were sequenced and proteomics data were generated to identify the role of differentially expressed miRNA (DEmiRNA) in PPR virus (PPRV) infected lung and spleen tissues of sheep and goats. In lungs, 67 and 37 DEmiRNAs have been identified in goats and sheep, respectively. Similarly, in spleen, 50 and 56 DEmiRNAs were identified in goats and sheep, respectively. A total of 20 and 11 miRNAs were found to be common differentially expressed in both the species in PPRV infected spleen and lung, respectively. Six DEmiRNAs—miR-21-3p, miR-1246, miR-27a-5p, miR-760-3p, miR-320a, and miR-363 were selected based on their role in viral infections, apoptosis, and fold change. The target prediction analysis of these six selected DEmiRNAs from the proteome data generated, revealed involvement of more number of genes in lung and spleen of goats than in sheep. On gene ontology analysis of host target genes these DEmiRNAs were found to regulate several immune response signaling pathways. It was observed that the pathways viz. T cell receptor signaling, Rap1 signaling, Toll-like receptor signaling, and B cell receptor signaling governed by DEmiRNAs were more perturbed in goats than in sheep. The data suggests that PPRV-induced miR-21-3p, miR-320a, and miR-363 might act cooperatively to enhance viral pathogenesis in the lung and spleen of sheep by downregulating several immune response genes. The study gives an important insight into the molecular pathogenesis of PPR by identifying that the PPRV—Izatnagar/94 isolate elicits a strong host response in goats than in sheep.

show abstract

Section: Introductionmentioning

confidence: 99%

Modulation of Host miRNAs Transcriptome in Lung and Spleen of Peste des Petits Ruminants Virus Infected Sheep and Goats

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…The number of DEGs in Crossbred were found to be more than in Tharparkar suggesting a greater dysregulation in systems biology in Crossbred. A contrast in gene expression was observed with 18.5 % of upregulated genes in Crossbred (Vrindavani cattle) downregulated in Tharparkar and 17.5% upregulated genes in Tharparkar downregulated in Crossbred. The increased HSPs levels have been found positively correlated with tolerance in many species.…”

mentioning

confidence: 91%

“…The systems biology behind a phenotype is most commonly studied by transcriptome profiling [14,15]. RNA-seq is a high throughput approach to measure relative global changes in the transcripts under specific condition(s) [14,16,17]. RNA -seq allows for analysis of transcriptome in an unbiased way, with, a tremendous dynamic detection range (>8,000 fold), and low background signals [18].…”

Section: Introductionmentioning

confidence: 99%

Systems Biology under heat stress in Indian Cattle

Khan

Sahu

Malla

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

BackgroundHeat stress induced by high environmental temperature and humidity affects livestock production and health. With global warming on the uprise, indigenous cattle known for their heat tolerance are gaining importance than the crossbreds. However, systems biology behind this phenotype in indigenous cattle is less known. In this study using transcriptome analysis, we identified key molecules and pathways that may lead to the heat tolerance phenotype in indigenous cattle (Tharparkar breed) Results

show abstract

“…PPRV vaccine developed by continuous passage (N=59) of Sungri/96 strain in Vero cells is widely used throughout India [4]. The vaccine provides long-lasting robust innate and adaptive -humoral and a strong cell-mediated immunity [2],which, however, warrants further investigation [5], [6], [7]. PPRV is lymphotropic and epitheliotropic [8], [9], [10].…”

Section: Introductionmentioning

confidence: 99%

“…Previously, RNA sequencing was used to unravel transcription factors, which modulate immune response to PPRV Sungri/96 live attenuated vaccine strain in vitro in PBMCs [6]. Also, a predicted immune signalling pathway of PPRV Sungri/96 vaccine induced immune response with predominant role of IRFs, TRIMs and ISGs in creation of robust antiviral state in vitro in PBMCs has been proposed [7].…”

Section: Introductionmentioning

confidence: 99%

Systems Biology behind immunoprotection of both Sheep and Goats after Sungri/96 PPRV vaccination

Wani

Praharaj

Sahu

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Immune response is a highly coordinated cascade involving all the subsets of PBMCs. In this study, RNA-Seq analysis of PBMC subsets - CD4+, CD8+, CD14+, CD21+ and CD335+ cells from day 0 and day 5 of Sungri/96 Peste des Petits Ruminants vaccinated sheep and goats was done to delineate the systems biology behind immune - protection of the vaccine in sheep and goat. Assessment of the immune response processes enriched by the differentially expressed genes in all the subsets suggested a strong dysregulation towards development of early inflammatory microenvironment, which is very much required for differentiation of monocytes to macrophages, and for activation and migration of dendritic cells into the draining lymph nodes. The protein - protein interaction networks among the antiviral molecules (IFIT3, ISG15, MX1, MX2, RSAD2, ISG20, IFIT5 and IFIT1) and common DEGs across PBMCs subsets in both the species identified ISG15 to be an ubiquitous hub, that helps in orchestrating antiviral host response against PPRV. IRF7 was found to be the key master regulator activated in most of the subsets in sheep and goats. Most of the pathways were found to be inactivated in B - lymphocytes of both the species indicating that 5 dpv is too early a time point for the B - lymphocytes to react. The cell mediated immune response and humoral immune response pathways were found more enriched in goats than in sheep. Though, animals from both the species survived the challenge, a contrast in pathway activation was observed in CD335+ cells.

show abstract

Comparative and temporal transcriptome analysis of peste des petits ruminants virus infected goat peripheral blood mononuclear cells

Cited by 30 publications

References 70 publications

Modulation of Host miRNAs Transcriptome in Lung and Spleen of Peste des Petits Ruminants Virus Infected Sheep and Goats

Modulation of Host miRNAs Transcriptome in Lung and Spleen of Peste des Petits Ruminants Virus Infected Sheep and Goats

Systems Biology under heat stress in Indian Cattle

Systems Biology behind immunoprotection of both Sheep and Goats after Sungri/96 PPRV vaccination

Contact Info

Product

Resources

About