Comparative ANNs with Different Input Layers and GA-PLS Study for Simultaneous Spectrofluorimetric Determination of Melatonin and Pyridoxine HCl in the Presence of Melatonin’s Main Impurity

Abstract

Melatonin (MLT) has many health implications, therefore it is important to develop specific analytical methods for the determination of MLT in the presence of its main impurity, N-{2-[1-({3-[2-(acetylamino)ethyl]-5-methoxy-1H-indol-2-yl}methyl)-5-methoxy-1H-indol-3-yl]ethyl}acetaamide (DMLT) and pyridoxine HCl (PNH) as a co-formulated drug. This work describes simple, sensitive, and reliable four multivariate calibration methods, namely artificial neural network preceded by genetic algorithm (GA-ANN), principal component analysis (PCA-ANN) and wavelet transform procedures (WT-ANN) as well as partial least squares preceded by genetic algorithm (GA-PLS) for the spectrofluorimetric determination of MLT and PNH in the presence of DMLT. Analytical performance of the proposed methods was statistically validated with respect to linearity, accuracy, precision and specificity. The proposed methods were successfully applied for the assay of MLT in laboratory prepared mixtures containing up to 15% of DMLT and in commercial MLT tablets with recoveries of no less than 99.00%. No interference was observed from common pharmaceutical additives and the results compared favorably with those obtained by a reference method.

Simultaneous determination of rutin and ascorbic acid mixture in their pure forms and combined dosage form

“…In addition, Several methods have been reported for simultaneous determination of mixture of these active ingredients like UV spectrophotometry [23,24], electrochemical method [25][26][27], chemiluminescence [28], capillary electrophoresis [29][30][31][32], high-performance liquid chromatography (HPLC) [33][34][35][36]and NIR FTIR [37]. The objective of this study is to develop a simple, rapid, sensitive and selective method for determination of rutin and ascorbic acid mixture in their pure forms and pharmaceutical dosage forms.…”

Development of Predictive Models for Initiation and Propagation of Field Transverse Cracking

“…The training data set is used to construct the model, and the testing data set is used to validate the model. The "leave one out cross-validation" (LOOCV) method is used in this study as it is always complete and every single data item can be used (27). Smaller PRESS values indicate a better predictive quality.…”

“…Specifically, (a) if more than the required number of predictor variables are selected, more noise will be added to the data and result in overfitting and (b) if the number of predictor variables is too small, meaningful data for model calibration might be discarded (27).…”

Genetic Algorithms-Based Approach for Wavelength Selection in Simultaneous Spectrofluorimetric Determination of Ofloxacin and Riboflavin in biological samples by Partial Least-Squares

“…The predictive ability for determination of pharmaceutical in biological samples with spectral overlapping improved by employing the proposed method. Several simultaneous determination methods based on the application of genetic algorithm have been reported [39][40][41][42][43].…”