Comparative anti-inflammatory effect of curcumin at air-liquid interface and submerged conditions using lipopolysaccharide stimulated human lung epithelial A549 cells

Hu, Yue; Sheng, Yunhua; Ji, Xiaoli; Liu, Ping; Tang, Liming; Chen, Gang; Chen, Guiliang

doi:10.1016/j.pupt.2020.101939

Cited by 15 publications

(10 citation statements)

References 49 publications

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“…A few other studies have also indicated larger effects in ALI compared to submerged cultures after exposure to different chemicals (not NPs), such as sodium metavanadate and carbendazim (Gohlsch et al 2019;Tollstadius et al 2019;Hu et al 2020). Additionally, Ohlinger et al (2019) have shown that culturing A549 cells at ALI increased the expression of several alveolar type II specific genes (for example SLCO2B1, ALB, CYP3A5, CFI) compared to when cultured in submerged, further indicating the advantages with ALI culture exposures, which more readily allows the effects of NPs on alveolar type II cell behavior.…”

Section: Discussionmentioning

confidence: 97%

Toxicological effects of zinc oxide nanoparticle exposure: an in vitro comparison between dry aerosol air-liquid interface and submerged exposure systems

et al. 2021

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Section: Discussionmentioning

confidence: 97%

Toxicological effects of zinc oxide nanoparticle exposure: an in vitro comparison between dry aerosol air-liquid interface and submerged exposure systems

et al. 2021

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“…Curcumin is primarily available in capsule form from commercial manufacturers [ 63 ] and the same as its oral absorption, the inhalation of free CUR is hampered because of its poor hydrophilicity. To overcome this, CUR has been derivatized it into a hydrophilic, nebulizable formulation [ 64 ] or it was loaded in liposomes [ 65 ] or polymeric and mesoporous microparticles [ 60 , 61 , 62 , 63 ] to be inhaled as dry powders. However, a nebulized CUR formulation is of interest, mainly because nebulizers are easy to use in infants, the elderly, patients with severely compromised lungs, and even ventilated patients.…”

Section: Discussionmentioning

confidence: 99%

“…However, a nebulized CUR formulation is of interest, mainly because nebulizers are easy to use in infants, the elderly, patients with severely compromised lungs, and even ventilated patients. In addition, nebulization is the aerosolization mechanism best suited to inhale liposomes [ 64 ]. Liposomes, however, have previously shown not to be well suited to retain CUR, which in biological media, tends to be easily released [ 65 ].…”

Section: Discussionmentioning

confidence: 99%

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Reparation of an Inflamed Air-Liquid Interface Cultured A549 Cells with Nebulized Nanocurcumin

et al. 2021

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The anti-inflammatory, antifibrotic and antimicrobial activities of curcumin (CUR) are missed because of its low solubility in aqueous media, low bioavailability, and structural lability upon oral intake. Soft nanoparticles such as nanoliposomes are not efficient as CUR carriers, since crystalline CUR is expelled from them to physiological media. Nanostructures to efficiently trap and increase the aqueous solubility of CUR are needed to improve both oral or nebulized delivery of CUR. Here we showed that SRA1 targeted nanoarchaeosomes (nATC) [1:0.4 w:w:0.04] archaeolipids, tween 80 and CUR, 155 ± 16 nm sized of −20.7 ± 3.3 z potential, retained 0.22 mg CUR ± 0.09 per 12.9 mg lipids ± 4.0 (~600 μM CUR) in front to dilution, storage, and nebulization. Raman and fluorescence spectra and SAXS patterns were compatible with a mixture of enol and keto CUR tautomers trapped within the depths of nATC bilayer. Between 20 and 5 µg CUR/mL, nATC was endocytosed by THP1 and A549 liquid–liquid monolayers without noticeable cytotoxicity. Five micrograms of CUR/mL nATC nebulized on an inflamed air–liquid interface of A549 cells increased TEER, normalized the permeation of LY, and decreased il6, tnfα, and il8 levels. Overall, these results suggest the modified pharmacodynamics of CUR in nATC is useful for epithelia repair upon inflammatory damage, deserving further deeper exploration, particularly related to its targeting ability.

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“…Combination of both endothelial and alveolar components allows optimal assessment of the cell-cell interaction during pulmonary inflammation ( 114 , 123 ). For instance, the impact of bacterial component lipopolysaccharide (LPS) ( 124 , 125 ) or human inflammatory cytokines like initerferon (IFN) or TNF ( 118 ) on the barrier is evaluated by ALI model. In such models, it is possible to include leukocytes in addition to the alveolar cells ( 126 , 127 ) and endothelial cells in a triple culture to optimally mimic disruptive barrier conditions during inflammation ( 120 , 128 ).…”

Section: Alveolocapillary In Vitro Model (Air-liqu...mentioning

confidence: 99%

A Barrier to Defend - Models of Pulmonary Barrier to Study Acute Inflammatory Diseases

et al. 2022

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Pulmonary diseases represent four out of ten most common causes for worldwide mortality. Thus, pulmonary infections with subsequent inflammatory responses represent a major public health concern. The pulmonary barrier is a vulnerable entry site for several stress factors, including pathogens such as viruses, and bacteria, but also environmental factors e.g. toxins, air pollutants, as well as allergens. These pathogens or pathogen-associated molecular pattern and inflammatory agents e.g. damage-associated molecular pattern cause significant disturbances in the pulmonary barrier. The physiological and biological functions, as well as the architecture and homeostatic maintenance of the pulmonary barrier are highly complex. The airway epithelium, denoting the first pulmonary barrier, encompasses cells releasing a plethora of chemokines and cytokines, and is further covered with a mucus layer containing antimicrobial peptides, which are responsible for the pathogen clearance. Submucosal antigen-presenting cells and neutrophilic granulocytes are also involved in the defense mechanisms and counterregulation of pulmonary infections, and thus may directly affect the pulmonary barrier function. The detailed understanding of the pulmonary barrier including its architecture and functions is crucial for the diagnosis, prognosis, and therapeutic treatment strategies of pulmonary diseases. Thus, considering multiple side effects and limited efficacy of current therapeutic treatment strategies in patients with inflammatory diseases make experimental in vitro and in vivo models necessary to improving clinical therapy options. This review describes existing models for studyying the pulmonary barrier function under acute inflammatory conditions, which are meant to improve the translational approaches for outcome predictions, patient monitoring, and treatment decision-making.

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Comparative anti-inflammatory effect of curcumin at air-liquid interface and submerged conditions using lipopolysaccharide stimulated human lung epithelial A549 cells

Cited by 15 publications

References 49 publications

Toxicological effects of zinc oxide nanoparticle exposure: an in vitro comparison between dry aerosol air-liquid interface and submerged exposure systems

Toxicological effects of zinc oxide nanoparticle exposure: an in vitro comparison between dry aerosol air-liquid interface and submerged exposure systems

Reparation of an Inflamed Air-Liquid Interface Cultured A549 Cells with Nebulized Nanocurcumin

A Barrier to Defend - Models of Pulmonary Barrier to Study Acute Inflammatory Diseases

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