Comparative Aspects of Certain Cancers

Stewart, Harold L.

doi:10.1007/978-1-4684-2736-3_10

Cited by 13 publications

(6 citation statements)

References 62 publications

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“…Little or no information on the malignant behavior, that is, invasion and metastasis, of the lesions was given. Others expressed the view that many of the &dquo;hepatomas&dquo; were carcinomas (36,42). The (50) was confirmed by Jones and Butler (22).…”

Section: Historical Backgroundsupporting

confidence: 55%

A Review of the Hepatic Tumors Related to Mixed-Function Oxidase Induction in the Mouse

Butler

1996

Toxicol Pathol

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Section: Historical Backgroundsupporting

confidence: 55%

A Review of the Hepatic Tumors Related to Mixed-Function Oxidase Induction in the Mouse

Butler

1996

Toxicol Pathol

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“…Epithelial HBs include fetal and embryonal subtypes [8]. Mouse HBs occur spontaneously in aged mice and can be induced by certain chemicals in several strains [4,15,20,21]. We have previously reported mouse HBs induced by DEN and PB [9], which are similar to those of embryonal type seen in man.…”

Section: Discussionmentioning

confidence: 79%

“…Cells were plated at 1 × 10 5 cells/dish in 35 mm plastic dishes in control medium alone or supplemented with MITO, hepatocyte growth factor (HGF; 10 ng/ml, Toyobo, Tokyo, Japan) or insulin/transferrin (each, 5 µg/ml, Sigma, Mouse hepatoblastomas (HBs) occur spontaneously in aged animals and also can be induced by certain chemicals in some strains [4,20,21]. Distinct histological, ultrastractural and immunohistochemical characteristics of mouse HBs have been described [3,4,15].…”

Section: Hematoxilin and Eosin (He)mentioning

confidence: 99%

Establishment and Characterization of a Cell Line from a Chemically-induced Mouse Hepatoblastoma

Kobayashi

Sakairi

Goto

et al. 2000

The Journal of Veterinary Medical Science

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ABSTRACT. We established a cell line (MHB-2) from a hepatoblastoma (HB) induced by diethylnitrosamine (DEN) and sodium phenobarbital (PB) in male B6C3F 1 mice and examined the biological characteristics of MHB-2. MHB-2 cells grew as monolayers in culture and showed a spindle or polygonal shape. Immunohistochemically, the original tumor cells and MHB-2 cells were negative for keratin, alphafetoprotein and albumin. Electron microscopically, MHB-2 cells had irregular-shaped nuclei with prominent nucleoli, abundant free ribosomes, myelinosomes, desmosomes and surface microvilli. Growth of this cell line was significantly accelerated by hepatocyte growth factor (HGF) and expression of its receptor c-met was confirmed by the reverse transcription-polymerase chain reaction (RT-PCR). MHB-2, however, was not found to be tumorigenic when transplanted into the subcutaneous tissue of syngeneic, nude or scid mice. To our knowledge, this is the first report on the establishment of a cell line derived from a mouse HB. MHB-2 would be useful for further studies to clarify the biological characteristics of mouse HB. hematoxilin and eosin (HE).Cell culture: Fresh tumor tissues were cut into small pieces, minced in Dulbecco's modified Eagle's medium (DMEM) (Nissui Pharmaceutical, Tokyo, Japan) with sterile scissors and washed with DMEM. The tumor pieces were treated with dispase (50 U/ml; Godo Shusei, Tokyo) for 30 min at 37°C and large tumor pieces were allowed to settle. Supernatant fluid containing cell clumps was collected after centrifugation at 100 g and cell pellets were resuspended in DMEM containing 10% fetal bovine serum (FBS; GIBCO, Grand Island, NY, U.S.A.) plus serum extender MITO (0.1%, Collaborative Biochemical Products, Bedford, MA, U.S.A.), 100 units/ml penicillin (GIBCO), and 100 units/ml streptomycin (GIBCO) and cultured on dishes coated with collagen type I (Iwaki, Tokyo) in a humidified 5% CO2 incubator at 37°C with weekly changes of the medium. After 2 months, growing colonies were harvested with trypsin/EDTA and passaged several times. Any remaining fibroblasts were removed by mechanical scraping and the differential attachment selection method, after which cultures of the cells forming epithelial-like monolayers were obtained (MHB-2 cell line). MITO contains insulin, transferrin, epidermal growth factor, endothelial growth supplement, dexamethasone and triiodo-17β-estradiol. The absence of Mycoplasma pulmoris contamination of the MHB-2 cell line was confirmed by the direct agar method.Growth characteristics: To evaluate the influence of growth factors on cell proliferation of the cell line, DMEM with 10% FBS in the absence of MITO was used as a control medium. Cells were plated at 1 × 10 5 cells/dish in 35 mm plastic dishes in control medium alone or supplemented with MITO, hepatocyte growth factor (HGF; 10 ng/ml, Toyobo, Tokyo, Japan) or insulin/transferrin (each, 5 µg/ml, Sigma, Mouse hepatoblastomas (HBs) occur spontaneously in aged animals and also can be induced by certain chemicals in some strains [4,...

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“…N early all types of primary liver tumors known to occur in humans can be reproduced by chemicals in laboratory animals, especially in rats. 1 In experimental carcinogenesis, preneoplastic foci of altered hepatocytes emerge weeks or months before the appearance of hepatocellular adenomas and hepatocellular carcinomas (HCCs), 2,3 and this has also been discovered in humans with hepatocellular neoplasms and/or cirrhosis. 4 This fact has led to the development of a number of in vivo systems for the study of early neoplasia in rat liver.…”

mentioning

confidence: 99%

The in vivo apoptotic effect of interferon alfa-2b on rat preneoplastic liver involves Bax protein

Álvarez¹,

Cerliani²,

Monti³

et al. 2002

Hepatology

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To determine whether interferon alfa (IFN-␣) prevents in vivo oncogenesis in very-earlystage cancer cells, we evaluated the action of IFN-␣2b over preneoplastic foci in rats. Animals were divided into 6 groups: subjected to a 2-phase model (diethylnitrosamine [DEN] plus 2-acetylaminofluorene [2-AAF]) of preneoplasia development (group 1), treated with IFN␣2b during the 2 phases (group 2), only during initiation with DEN (group 3), only during administration of 2-AAF (group 4), subjected only to an initiation stage (group 5), and treated with IFN-␣2b during this period (group 6). The numbers of placental form of rat glutathione S-transferase (rGST-P)-positive foci per liver and the foci as percentage of liver were significantly reduced in groups 2, 3, and 6 but not in group 4. Rats treated with IFN-␣2b showed a higher apoptotic index (AI) in altered hepatic foci (AHF). Levels of p53 and Bax protein in liver lysates were significantly increased in those animals. Similarly, levels of antiapoptotic proteins Bcl-2 and Bcl-x L in mitochondrial fraction were decreased. Finally, increased levels of Bax protein were localized in the mitochondria of rats that received IFN-␣2b, at least during the DEN phase (groups 2, 3, and 6), whereas mitochondrial Bax expression was not increased in group 4. In conclusion, the preneoplastic hepatocytes in rats that received IFN-␣2b during the initiation stage undergo programmed cell death as a primary result of a significant increase in the amount and translocation to the mitochondria of Bax protein. (HEPATOLOGY 2002;35:824-833.)

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Comparative Aspects of Certain Cancers

Cited by 13 publications

References 62 publications

A Review of the Hepatic Tumors Related to Mixed-Function Oxidase Induction in the Mouse

A Review of the Hepatic Tumors Related to Mixed-Function Oxidase Induction in the Mouse

Establishment and Characterization of a Cell Line from a Chemically-induced Mouse Hepatoblastoma

The in vivo apoptotic effect of interferon alfa-2b on rat preneoplastic liver involves Bax protein

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