Comparative aspects of murine proteinase 3

Relle, Manfred; Thomaidis, Thomas; Galle, Peter R.; Schwarting, Andreas

doi:10.1007/s00296-010-1672-2

Cited by 7 publications

(4 citation statements)

References 34 publications

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“…None of the cells and organs expressed traceable quantities of a truncated isoform of PR3. Note, that breakdown products of PR3 are visible in the bone marrow lane, which was already described in Relle et al [11]. M: Protein ladder in kDa.…”

Section: Cell Sorting and Mrna Detectionsupporting

confidence: 60%

“…For the detection of murine PR3, harvested CMT 64/61, Meta 7, RAG, WEHI 274, and NIH-3T3 D4 as well as spleen, kidney, lung, and bone marrow cells were lysed and 30 mg of lysate was subjected to an 8% SDS-PAGE. Blotting with a rabbit anti-mouse PR3 peptide antibody was done as previously described [11].…”

Section: Immunoblot Of Murine Pr3 and Cdp (Ccaat Displacement Protein)mentioning

confidence: 99%

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Intronic promoters and their noncoding transcripts: A new source of cancer‐associated genes

Relle

Becker

Meyer

et al. 2012

Molecular Carcinogenesis

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Recent studies of mammalian genomes suggest that alternative promoters are associated with various disorders, including cancer. Here we present an intronic promoter of the murine proteinase 3 gene, which drives the expression of an alternative mRNA in intron 2 of the prtn3 gene. The proximal promoter sequences were identified and a series of promoter deletion constructs were used to identify the sequence elements that are required for basal promoter activity. Expression of the homeobox transcription factor CUX1 p75 isoform was found to suppress the activity of the alternative PR3 promoter. Data base analyses, multiple alignments and expression data showed that the intronic PR3 promoter is active in leukemia and other tumor cells as well as in mouse embryo, male mammary gland and bone marrow. In the spleen, the transcript is exclusively expressed by Gr-1(int) /CD11b(+) cells, which are also known as myeloid-derived suppressor cells (MDSCs). In humans, an alternative transcript of the PR3-gene could be detected in the bone marrow and in various cancer cell lines but not in primary leukemia cells, suggesting a species-overarching function of this kind of promoter. Therefore, the alternative PR3 promoter and its mRNA may be useful tools to investigate the fate of hematopoietic stem cells.

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Section: Cell Sorting and Mrna Detectionsupporting

confidence: 60%

Section: Immunoblot Of Murine Pr3 and Cdp (Ccaat Displacement Protein)mentioning

confidence: 99%

Intronic promoters and their noncoding transcripts: A new source of cancer‐associated genes

Relle

Becker

Meyer

et al. 2012

Molecular Carcinogenesis

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“…There is, however, increasing evidence of major functional differences between the human and murine immune systems, indicating limited application of data obtained in studies employing murine cells or models ( 5 , 6 ). On one hand, the composition of the murine leukocyte repertoire differs from that of humans; in mice only 7–28% of peripheral blood leukocytes are PMNs in contrast to 35–70% in human blood ( 7 ). On the other hand, functional differences, such as the sequence of participating immunological cells after antigen challenge as well as the dose of antigen needed to initiate immunological reaction in delayed type hypersensitivity have been described ( 5 ).…”

Section: Introductionmentioning

confidence: 99%

Human and Murine Innate Immune Cell Populations Display Common and Distinct Response Patterns during Their In Vitro Interaction with the Pathogenic Mold Aspergillus fumigatus

et al. 2017

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Aspergillus fumigatus is the main cause of invasive fungal infections occurring almost exclusively in immunocompromised patients. An improved understanding of the initial innate immune response is key to the development of better diagnostic tools and new treatment options. Mice are commonly used to study immune defense mechanisms during the infection of the mammalian host with A. fumigatus. However, little is known about functional differences between the human and murine immune response against this fungal pathogen. Thus, we performed a comparative functional analysis of human and murine dendritic cells (DCs), macrophages, and polymorphonuclear cells (PMNs) using standardized and reproducible working conditions, laboratory protocols, and readout assays. A. fumigatus did not provoke identical responses in murine and human immune cells but rather initiated relatively specific responses. While human DCs showed a significantly stronger upregulation of their maturation markers and major histocompatibility complex molecules and phagocytosed A. fumigatus more efficiently compared to their murine counterparts, murine PMNs and macrophages exhibited a significantly stronger release of reactive oxygen species after exposure to A. fumigatus. For all studied cell types, human and murine samples differed in their cytokine response to conidia or germ tubes of A. fumigatus. Furthermore, Dectin-1 showed inverse expression patterns on human and murine DCs after fungal stimulation. These specific differences should be carefully considered and highlight potential limitations in the transferability of murine host–pathogen interaction studies.

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“…The difficulties in developing animal models for PR3-ANCA vasculitis are in part due to important species differences with respect to expression and physicochemical properties of PR3 [37,38]. To circumvent these differences, current strategies aim to develop humanized experimental model systems.…”

Section: Animal Models For Pr3-anca Vasculitismentioning

confidence: 99%