Comparative aspects of zebrafish (Danio rerio) as a model for aging research

Gerhard, Glenn S.

doi:10.1016/j.exger.2003.10.022

Cited by 76 publications

(39 citation statements)

References 93 publications

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“…В регуляции метаболизма глюкозы инсулином у зебрафиш и млекопитающих есть много общего. Основные белки -участники инсулинсигнальной системы, по-видимому, структурно и функционально близки к таковым у млекопитающих [44]. Показано, что чувствительность взрослых особей зебрафиш к действию антидиабетических препаратов подобна таковой у млекопитающих.…”

Section: использование зебрафиш для моделирования болезней человекаunclassified

Zebrafish as a model system for biomedical studies

et al. 2010

BIOMED KHIM

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Ключевые слова: зебрафиш (Danio rerio), разработка лекарств, острая токсичность, тератогенность, апоптоз, рак.ВВЕДЕНИЕ. Биомедицинские исследования, направленные на понимание патогенеза заболеваний человека на клеточном и молекулярном уровне и создание новых терапевтических средств, основаны на использование различных моделей животных. Зебрафиш (Danio rerio) -маленькая (размером до 4,5 см) пресноводная тропическая рыбка, обитающая в Индии и Южной Азии и хорошо известная любителям аквариумных рыб. Традиционно эту рыбку использовали в исследованиях в области молекулярной генетики и биологии развития, однако в последнее время она становится привлекательной в работах по созданию новых лекарственных препаратов и моделировании различных физиологических и патологических процессов [1][2][3][4][5][6].Эмбрион зебрафиш развивается быстро. Уже через три дня после оплодотворения у него функционирует сердце, кровеносная и нервная системы. Через четыре дня появляются личинки (мальки), способные есть и плавать. Эпителиальные клетки кишечника выделяют пищеварительные ферменты, гепатоциты секретируют желчь, панкреатические клетки продуцируют инсулин и карбоксипептидазы. Так как эмбрионы прозрачны, за их развитием можно наблюдать визуально с помощью микроскопа. Эмбрионы зебрафиш легко поглощают низкомолекулярные соединения из воды через кожу и жабры, а после 7 суток развития поглощение происходит через рот (а не через кожу) [7].Срок жизни полосатого Danio 3-5 лет. 120 * -адресат для переписки

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Section: использование зебрафиш для моделирования болезней человекаunclassified

Zebrafish as a model system for biomedical studies

et al. 2010

BIOMED KHIM

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“…Recently, it has also attracted attention as a model for studying aging [ 8,13,14,27,31 ]. Under laboratory conditions, zebrafish mature within the first 6 months and survive for up to 6 years.…”

Section: Introductionmentioning

confidence: 99%

Aging of the circadian system in zebrafish and the effects of melatonin on sleep and cognitive performance

Zhdanova

López‐Patiño

et al. 2008

Brain Research Bulletin

132

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Aging is a complex process involving intracellular changes and, notably, modifications in intercellular communications, required for coordinated responses to internal and external events. One of the potential reasons for such changes is an age-dependent failure of the integrating systems, including the circadian clock. Here we demonstrate that aging in a diurnal vertebrate, zebrafish (Danio rerio), is associated with major but selective circadian alterations. By 3-5 years of age, zebrafish have reduced amplitude and increased fragmentation of entrained circadian rhythms of activity, with fast desynchronization of the rhythms in the absence of environmental time cues. Aging in zebrafish is also associated with a reduction in the overall duration of nighttime sleep, followed by lower activity levels and a higher arousal threshold during the day. The production of the principal circadian hormone, melatonin, progressively declines during zebrafish aging. However, the ability of melatonin to acutely promote sleep and entrain circadian rhythms of activity remains robust until at least 4-5-years of age, consistent with the preserved levels of mRNA expression for melatonin receptors. Aged zebrafish have altered expression of the circadian genes zBmal1 and zPer1 but not zClock1. A lack of circadian time cues alters cognitive performance in aged more than in young zebrafish and this can be partially attenuated by daily melatonin administration. The advantages of zebrafish as a diurnal, small, prolific and genetically well-characterized vertebrate model provide new opportunities to clarify the intrinsic circadian factors involved in human aging and promote the search for prophylactic and treatment strategies.

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“…31 The lifespan of inbred zebrafish is reduced by 10%-15% compared to that of outbred zebrafish. 31 Furthermore, zebrafish lose their reproductive function after 2 years; 32 therefore, the electrical impedance of zebrafish cells older than 24 months could be affected by hormonal changes or by epizootic disease. Thus, the older group consisted of healthy 18-month-old zebrafish for appropriate comparison with the younger group.…”

Section: à3mentioning

confidence: 99%

Ex vivo characterization of age-associated impedance changes of single vascular endothelial cells using micro electrical impedance spectroscopy with a cell trap

et al. 2016

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We aimed to characterize aging of single vascular endothelial cells, which are indicators of senescence, using micro electrical impedance spectroscopy (lEIS) for the first time. The proposed lEIS was equipped with two barriers under the membrane actuator near the sensing electrodes, increasing its cell-trapping capability and minimizing the interference between the target cell and subsequent cells. The cell-trapping capability in lEIS with barriers was considerably improved (90%) with a capture time of 5 s or less, compared to lEIS without barriers (30%). Cells were extracted from transgenic zebrafish to minimize an initial discrepancy originating from genetic differences. In order to estimate useful parameters, cytoplasm resistance and membrane capacitance were estimated by fitting an electrical equivalent circuit to the data of ex vivo sensor output. The estimated cytoplasm resistance and membrane capacitance in the younger vascular endothelial cells were 20.16 6 0.79 kX and 17.46 6 0.76 pF, respectively, whereas those in the older cells were 17.81 6 0.98 kX and 20.08 6 1.38 pF, respectively. Discrimination of each group with different aging showed statistical significance in terms of cytoplasm resistance (p < 0.001) and membrane capacitance (p < 0.001). Considering both of the sensor and cellular level, the optimal frequency was determined as 1 MHz at which the electrical impedance of each group was clearly discriminated (p < 0.001). V C 2016 AIP Publishing LLC.

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Comparative aspects of zebrafish (Danio rerio) as a model for aging research

Cited by 76 publications

References 93 publications

Zebrafish as a model system for biomedical studies

Zebrafish as a model system for biomedical studies

Aging of the circadian system in zebrafish and the effects of melatonin on sleep and cognitive performance

Ex vivo characterization of age-associated impedance changes of single vascular endothelial cells using micro electrical impedance spectroscopy with a cell trap

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