Comparative assessment of detached leaflet and tuber disc assays for studying the aggressiveness of different isolates of Phytophthora infestans

Majeed, Abdul; Muhammad, Zahir; Ullah, Rehan; Chaudhry, Zubeda; Haq, Irfanul

doi:10.30848/pjb2019-2(35)

Cited by 5 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previously, it was demonstrated that laboratory studies through detached leaf assay for assessing late blight disease is a quick and accurate method [46,47]. Tian et al [15] explained P. infestans inoculation on detached leaves of RNAi transgenic plant at 4dpi and 5dpi with lesser disease symptoms than the control plant.…”

Section: Discussionmentioning

confidence: 99%

Functional inhibition of the StERF3 gene by dual targeting through CRISPR/Cas9 enhances resistance to the late blight disease in Solanum tuberosum L.

et al. 2022

View full text Add to dashboard Cite

BackgroundThe use of disease-resistant cultivars is the best solution to get their maximum yield potential and avoid fungicide application. There is no doubt about the contribution, and use of R genes (resistance genes) in resistance development in plants, while S genes (susceptibility genes) also hold a strong position in pathogenesis by resistance repression, and their loss of function contributes to enhanced resistance.Hence, we attempted to knock out the function of StERF3 in potatoes through CRISPR/Cas9 based genome editing and investigated the CRISPR/Cas9 approach as strategic control against late blight disease in potato plants. Methods and resultsThe StERF3 gene was edited in late blight susceptible cv. Lady Rosetta. Full allelic edited plants were identi ed through DnpI and N1aIV mediated restriction digestion and then further analyzed through Indel Detection by Amplicon Analysis. Sequence analysis of targeted plants for indel identi cation showed full allelic editing. The detached leaf assay of full allelic edited plants demonstrated the role of the StERF3 gene in susceptibility to late blight in potatoes. In planta disease assay also showed reduced, slowed, and delayed disease progression in StERF3-loss-of-function mutants in comparison to wild-type (control) plants. Less fungal biomass was quanti ed in knockouts through Real-time qPCR that supported less susceptibility of edited plants to late blight. Besides, relatively high expression of pathogens-related genes, StPR1, and StNPR1 were also observed in StERF3-loss-of-function mutants compared to the corresponding control. ConclusionThe results showed the functional inhibition of StERF3 genes using the CRISPR/Cas9 approach. The functional knockouts of StERF3 generated potato plants revealed enhanced resistance against Phytophthora infestans and thereby demonstrated as best strategic control for late blight disease in potato plants.

show abstract

Section: Discussionmentioning

confidence: 99%

Functional inhibition of the StERF3 gene by dual targeting through CRISPR/Cas9 enhances resistance to the late blight disease in Solanum tuberosum L.

et al. 2022

View full text Add to dashboard Cite

show abstract

“…In Pakistan, potato area, production and average yield have been 174.4 thousand hectares, 4,245.9 thousand tones, and 24.3 t/ha, respectively during 2017–18 ( Ijaz-ul-Hassan, Khan & Hussain, 2022 ). The potato is used as a staple food in many countries around the world, feeding more than 10 million people ( Majeed & Muhammad, 2018 ). All essential elements, including carbohydrates, amino acids, several vitamins (such as vitamin C and vitamin A), numerous minerals including (potassium, magnesium, and iron), beneficial phytochemicals (such as phenolics, flavonoids, and carotenoids), and dietary fiber (skin) can be full filed by potato tubers.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of Pectobacterium atrosepticum infecting potato and its management through chemicals

Hameed,

Zeeshan,

Binyamin

et al. 2024

PeerJ

View full text Add to dashboard Cite

Potato farming is a vital component of food security and the economic stability especially in the under developing countries but it faces many challenges in production, blackleg disease caused by Pectobacterium atrosepticum (Pa) is one of the main reason for damaging crop yield of the potato. Effective management strategies are essential to control these losses and to get sustainable potato crop yield. This study was focused on characterizing the Pa and the investigating new chemical options for its management. The research was involved a systematic survey across the three district of Punjab, Pakistan (Khanewal, Okara, and Multan) to collect samples exhibiting the black leg symptoms. These samples were analyzed in the laboratory where gram-negative bacteria were isolated and identified through biochemical and pathogenicity tests for Pa. DNA sequencing further confirmed these isolates of Pa strains. Six different chemicals were tested to control blackleg problem in both vitro and vivo at different concentrations. In vitro experiment, Cordate demonstrated the highest efficacy with a maximum inhibition zones of 17.139 mm, followed by Air One (13.778 mm), Profiler (10.167 mm), Blue Copper (7.7778 mm), Spot Fix (7.6689 mm), and Strider (7.0667 mm). In vivo, Cordate maintained its effectiveness with the lowest disease incidence of 14.76%, followed by Blue Copper (17.49%), Air One (16.98%), Spot Fix (20.67%), Profiler (21.45%), Strider (24.99%), and the control group (43.00%). The results highlight Cordate’s potential as a most effective chemical against Pa, offering promising role for managing blackleg disease in potato and to improve overall productivity.

show abstract

“…Stimulation effects are observed when plants are irradiated at doses determined for each species and depend on their physiological state, for example, for wheat, the stimulating dose of gamma radiation for wheat ranges from 5Gy to 8Gy; for barley, it varies from 10Gy to 30Gy [12][13][14]. The manifestation of stimulating effects after radiation treatment occurs along molecular pathways similar to other stressors [15], when low doses of gamma radiation become plant growth stimulants [16].…”

Section: Introductionmentioning

confidence: 99%

Developing the Adapted Scale of Microphenological Phases for the Controlled Sprouting of Grain Crops

Timakova¹,

Iliukhin²

2023

E3S Web Conf.

View full text Add to dashboard Cite

As a result of the research and in view of the methodological approach to the controlled sprouting of grain crops, the term “sprouted grain” adapted to the conditions of controlled sprouting has been proposed. A unified scale of microphenological phases for controlled sprouting of different types of grain crops treated with low doses of gamma radiation has been developed. In order to take the reading for the quiescence state of the grains of the “Ekaterina” soft spring wheat and the “Remembrance of Chepelev” spring barley of 2019-2021 harvest years zoned in the Ural region, the treatment with low doses of ionizing gamma radiation (5Gy, 10Gy, and 15Gy) has been carried out. In the developed scale of microphenological phases for germination of grain crops, the dry grain phase has been introduced and the technological features of the sprouting and its effectiveness have been given. In the second phase of the sprouting, intense water absorption is observed in all groups of samples. Bioactivation of grain crops effects the increase in germination energy—up to 92-93% when treated with a dose of 10Gy. The germination rate of the 1st seed is on average 2-3 days. Low-dose gamma radiation of grains is distinguished by its industrial applicability when sprouted grains are used as an independent product or as a raw material component.

show abstract

Comparative assessment of detached leaflet and tuber disc assays for studying the aggressiveness of different isolates of Phytophthora infestans

Cited by 5 publications

References 0 publications

Functional inhibition of the StERF3 gene by dual targeting through CRISPR/Cas9 enhances resistance to the late blight disease in Solanum tuberosum L.

Functional inhibition of the StERF3 gene by dual targeting through CRISPR/Cas9 enhances resistance to the late blight disease in Solanum tuberosum L.

Molecular characterization of Pectobacterium atrosepticum infecting potato and its management through chemicals

Developing the Adapted Scale of Microphenological Phases for the Controlled Sprouting of Grain Crops

Contact Info

Product

Resources

About