Comparative assessment of genetic diversity among Indian bamboo genotypes using RAPD and ISSR markers

Abstract

Bamboo is one of the important plant for pulp, paper and charcoal industries. After China, India is the second largest bamboo reserve in Asia. Around the globe, wide genetic diversity of bamboo is present which serves as the base for selection and improvement. DNA based molecular markers appears to be a striking substitute for systematic assessment of the genetic diversity in conservation and genetic improvement of plants. DNA based molecular markers such as RAPD and ISSR were used to assess the genetic diversity in 13 bamboo genotypes. Total 120 RAPD and 63 ISSR primers were tested, of which only 42 polymorphic primers (30 RAPD and 12 ISSR), gave reproducible amplification profile and were used in this study. 30 RAPD primers yielded total 645 amplified fragments, of which 623 were polymorphic, and 20.76 polymorphic bands per primer were observed across 13 genotypes. 12 ISSR primers produced 246 amplified fragments, of which 241 were polymorphic, and 20.08 polymorphic bands per primer was observed across 13 different genotypes. The Jaccard's coefficient of RAPD, ISSR and pooled RAPD and ISSR dendrograms ranged from 0.26 to 0.83, 0.23 to 0.86 and 0.26 to 0.84 respectively. The present study found the large genetic diversity present between different elite genotypes of bamboo. Such investigation can deliver a well understanding of the available genotypes, which might be further exploited for the paper industry.

RAPD and ISSR Marker-Based Comparative Evaluation of Genetic Diversity Among Indian Germplasms of Euryale ferox: an Aquatic Food Plant

“…Therefore, in the present investigation, RAPD and ISSR were used as genetic markers to characterize the 16 germplasms of E. ferox. In the last three decades, both ISSR and RAPD markers have played an important role in genetic variability studies of different populations [11,12,22,23]. In the present investigation, 21 ISSR and 40 RAPD primers produced 747 polymorphic bands that clearly discriminated 16 E. ferox germplasms into three main clusters.…”

“…Among different DNA-based markers, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers are inexpensive and involve only a small amount of DNA sample without need of radioactive labelling. The RAPD marker has been utilized in detecting genetic variability in several species of plants [8][9][10][11][12]. The ISSR marker has been also proven to provide a strong, reproducible, easy, fast and economical means to evaluate genetic variability and identify variations between narrowly associated genotypes in several species of plants [11][12][13][14][15][16].…”

ISSR Marker Based Population Genetic Study ofMelocanna baccifera(Roxb.) Kurz: A Commercially Important Bamboo of Manipur, North-East India

“…The results from the Mantel test also supported the above observation as it showed no correlation between the genetic and geographic distances between different populations. The ultimate goal of conservation is to ensure the continuous survival of population and to maintain their evolutionary potential by preserving natural levels of genetic diversity within and between populations [9, 56, 57]. The natural M. baccifera resources in the selected sites have been exploited by locals for many years leading to loss of plant resources, habitat destruction, and fragmentation.…”

Evaluation of genetic diversity of Clinacanthus nutans (Acanthaceaea) using RAPD, ISSR and RAMP markers

“…RAPD has been successfully applied in genetic variation investigations of Mucuna pruriens due to its usefulness as an informative and cheap tool for plant breeding programs (Patil et al 2016). There are also many researchers using these markers as one of their techniques to study the genetic diversity of their desired plant species (Desai et al 2015;Kumar et al 2009Kumar et al , 2010Mei et al 2015;Patel et al 2015;Patil et al 2016;Pu et al 2009;Saleh 2015;Zhao et al 2013;Zhao et al 2015). RAPD and ISSR are dominant markers that function well in DNA fingerprinting studies of plant species.…”