Comparative assessment of Helicobacter pylori colonization in children tonsillar tissues

Agreement rate of rapid urease test, conventional PCR, and scorpion real-time PCR in detecting Helicobacter pylori from tonsillar samples of patients with chronic tonsillitis

“…These results are consistent with those reported by Jabbari-Moghaddam et al .,[29] in which a low efficacy of RUT in detecting H. pylori in tonsillar biopsy specimens was obtained. In addition, our data regarding the existence of a strong agreement between the conventional PCR and Scorpion real-time PCR are also in harmony with the results described by Burucoa et al .,[28] Liu et al .,[26] Ben Mansour et al .,[25] and Qiu et al .…”

“…The results reported by Jabbari Moghaddam et al ., confirm the ability of urease production by other bacterial populations in such anatomical areas. [29] Moreover, the ureC gene target in conventional PCR, which identifies the presence of the urease enzyme is also of inadequate specificity. [30] Above all, the negative culture result seen in the H. pylori -infected tonsillar samples,[31] is regarded as another hampering factor in the application of RUT and conventional PCR as the standard tests.…”

State of the globe: Diagnostic tests to detect Helicobacter pylori tonsillitis

“…[1920] Urease production by other bacterial species colonized in tonsil, throat, and mouth is documented; this can interfere with the RUT, leading to low specificity for the detection of H. pylori . [21] Yilmaz et al . used the CLO-test on the adenotonsillectomy specimens obtained from 50 children; they found no infection in the specimens, though the H. pylori Ag was detected in the stools of 50% of the children.…”

“…[31] The scorpion real-time PCR has been used by a number of researchers. [1213213233] Utilization of the conventional PCR and the scorpion real time PCR in comparison with URT showed a strong agreement between PCR-based methods in detection of the bacterium DNA in the specimens of chronic tonsillitis and found the scorpion real-time PCR as a preferred method for diagnosis of such specimens. [34]…”

Codetecção de bactérias em pacientes com adenoamigdalite crônica

“…Infecções por vários tipos de microrganismos podem levar ao aumento do tecido de amígdala e adenoide, (Hellings et al, 2000;Brandtzaeg, 2003;Jabbari Moghaddam et al, 2009). Os antígenos são transportados através do epitélio pelas células membranosas (M) e atingem um tecido com altas concentrações de linfócitos, macrófagos e células dendríticas (Van Kempen et al, 2000).…”