Comparative assessment of immunodiffusion and enzyme-linked immunosorbent assay used for bovine leukosis diagnosis

Mustafayev, Arkif R.; Baratov, M.O.

doi:10.29326/2304-196x-2023-12-1-52-56

Veterinary Science Today

2023

DOI: 10.29326/2304-196x-2023-12-1-52-56

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Comparative assessment of immunodiffusion and enzyme-linked immunosorbent assay used for bovine leukosis diagnosis

Arkif R. Mustafayev

M.O. Baratov

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Cited by 3 publications

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Retrospective analysis of enzootic bovine leucosis spread in Republic of Dagestan considering natural and climatic conditions

Mustafayev,

Baratov

2023

Veterinariâ segodnâ

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Retrospective analysis of enzootic bovine leucosis (EBL) data received by the Republic of Dagestan Veterinary Laboratory and Veterinary Department of the Republic of Dagestan was made. From 1988 to 2022, the Republican veterinary laboratories serologically tested3,205,118 animal sera for the antibodies to bovine leukaemia virus (BLV) antigen, and 76,133 (2.4%) of them were positive. High BLV infection levels were detected in 1988 (32.2%), 1989 (21.3%), 1991 (23.3%), 1993 (23.0%), 2005 (24.2%), 2010 (23.0%), and the lowest ones were reported in the recent years: 2020 – 1.0%, 2021 – 1.0%, 2022 – 0.5%. In2022, diagnostic testing of 875,312 serum samples was carried out, which included 476,493 sera collected from bovines in high-altitude and mountainous areas of Dagestan. In the plain areas, 255,312 bovine animals were tested for leucosis, and 122,967 animals were tested in the sub-mountain areas. The animal infection with BLV in these natural and climatic conditions was reported as follows: high-altitude and mountainous areas– 0.5% (2,313 animals), plain areas– 0.8% (1,925 animals), sub-mountain areas– 0.1% (109 animals). Additional 20,540 serum samples were tested in the laboratories at the transhumance pasture veterinary units, and 170 BLV seropositive animals (0.83%) were detected. No EBL was diagnosed in the laboratories of the Derbent, Kochubeysk, Ulankholsk, Bakressk veterinary units, but other four laboratories detected high level of BLV seropositive animals (Kizlyarsk– 14.6%, Babayurt – 3.6%, Tarumovsk– 3.0%, Kyzylyurt– 1.06%). Thus, EBL is widespread in animals, especially in the plain areas of the Republic of Dagestan.

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Retrospective analysis of enzootic bovine leucosis spread in Republic of Dagestan considering natural and climatic conditions

Mustafayev,

Baratov

2023

Veterinariâ segodnâ

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Enzyme-linked immunosorbent assay for post-slaughter diagnosis of bovine leukosis

Mustafayev,

Baratov

2024

Veterinariâ segodnâ

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Postmortem diagnosis of enzootic bovine leukosis is made on the basis of the results of tests of biological materials from emergently slaughtered or fallen animals using pathomorphological, histological and molecular genetic methods that have some disadvantages. Results of post-slaughter diagnostic tests for bovine leukosis with enzyme-linked immunosorbent assay are described in the paper. For this purpose, 83 swabs were collected from different carcass parts including 71 swabs from carcasses of the animals that were not pre-slaughter tested and 12 samples from the carcasses of the animals that were pre-slaughter tested with immunodiffusion assay and found bovine leukemia virus-seronegative (control samples). Sterile scalpels, cotton wool, 5 mL tubes with caps were used for swab collection. The samples were taken from incisions in carcasses and internal organs of slaughtered animals with sterile cotton-wool swabs and placed in single-use tubes. Distilled water (or isotonic solution – 0.85% NaCl) was added to the tubes with samples, 0.1 to 0.2 mL per tube depending on the sample size, and the tubes were left at room temperature (22–26 °С) for 1.5–2.0 hours and regularly shaken. Resulting homogeneous substrate was used for enzyme-linked immunosorbent assay carried out in accordance with the instructions for the test-kit for detection of antibodies against bovine leukemia virus. Specific antibodies to bovine leukemia virus gp51 antigen were detected in 6 (8.5%) out of 71 swabs subjected to the laboratory tests. Therewith, the antibodies were detected only in 3 swabs (4.2%) when the swabs were tested with immunodiffusion assay. All 12 control samples from animals that were pre-slaughter tested and found seronegative were negative when tested with enzyme-linked immunosorbent assay. Therefore, the above-said serological method can be used for post-slaughter diagnosis of bovine leukosis together with conventional methods.

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The epizootic situation of bovine leukemia in the Republic of Dagestan in 2023

Mustafaev

2024

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Bovine enzootic leukemia (EBLV) remains an acute problem for animal husbandry in many regions of Russia. Based on this, the goal was set: to study the epizootic situation of bovine leukemia in the Republic of Dagestan. The material for the study was taken from the official source of the Veterinary Committee of the Republic, as well as supplemented with our own serological blood tests for bovine leukemia virus (bovine leukemia). Laboratory tests for the detection of antibodies to BLV in RID were carried out according to the "Guidelines for the diagnosis of bovine leukemia" (2000). Research results: the spread of BLV has been established in some municipal districts and urban districts throughout the republic. In 2023, from all veterinary laboratories (stations), serological studies were conducted in the amount of 1101,689 animal blood samples for BLV. Of the animals studied, antibodies to the BLV antigen in RID were obtained in 2,778 (0.25%) samples. A high percentage of animal HCV infection was detected in the following districts and cities: Kizlyarsky – 2% (388), Tlaratinsky – 1.3% (341), Botlikhsky – 1.2% (450), Babayurt – 1.1% (188), Gunibsky – 0.8% (228) and in Makhachkala – 0.9% (180). In 16 districts and in Khasavyurt, the percentage of infection of animals with BLV was at the level of 0.3% or lower. We obtained 440 animal blood samples from sample farms, which were examined in the RID. 37 (8.4%) animal blood samples gave a positive result for BLV. Thus, the general epizootic situation in EBLV remains relevant, but the prevalence of BLV among animals is decreasing in the republic.

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Comparative assessment of immunodiffusion and enzyme-linked immunosorbent assay used for bovine leukosis diagnosis

Cited by 3 publications

References 0 publications

Retrospective analysis of enzootic bovine leucosis spread in Republic of Dagestan considering natural and climatic conditions

Retrospective analysis of enzootic bovine leucosis spread in Republic of Dagestan considering natural and climatic conditions

Enzyme-linked immunosorbent assay for post-slaughter diagnosis of bovine leukosis

The epizootic situation of bovine leukemia in the Republic of Dagestan in 2023

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