Comparative Assessment of in Vitro Antimicrobial Activity of Curcuma Caesia Roxb. And Curcuma Amada Roxb

Abstract

Objective: The aim of the present study was to compare the in vitro antimicrobial activity of Curcuma caesia Roxb. and Curcuma amada Roxb. family Zingiberaceae. Both plants have been used traditionally for curing a number of diseases and ailments. The plants have been specifically used in skin problems and inflammatory conditions. Methods: Each plant material was extracted with dichloromethane (DCM) and ethanol. All extracts were subjected to preliminary phytochemical screening. The antibacterial activity of the extracts was tested against two Gram-positive (Staphylococcus aureus, Streptococcus pyogenes), and two Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa) using the cup-plate method. The standard drug used was ofloxacin (0.2 ml) at a concentration of 5 mg/ml. The antifungal activity was tested against Aspergillus fumigatus, and Candida albicans using the cup-plate method and clotrimazole (10 mg/ml) was taken as standard reference.Results: Phytochemical screening performed on the extracts has shown the presence of various constituents such as glycosides, carbohydrates, saponins, phytosterols, resins, flavonoids, and diterpenoids. On comparing both species, C. amada was found to be stronger growth inhibitor against both Gram-positive and Gram-negative strains. C. caesia ethanol extract at a dose of 200 mg/ml was most effective and C. amada DCM extract 100 mg/ml was least effective in case of antifungal activity against C. albicans. In case of A. fumigatus, C. caesia DCM extract 100 mg/ml was least effective and C. caesia ethanol extract 200 mg/ml was most effective. Conclusion:The present study provides the information on phytochemical screening and antimicrobial activities of extracts prepared from two plants of family Zingiberaceae, i.e., C. caesia Roxb. and C. amada Roxb.

Antimicrobial Assessment and Phytochemical Screening of Medicinal Plants and Ganoderma lucidum.

“…The resistance shown by E.coli could be attributed to its cell wall structure as it has an effective permeability barrier, comprised of a thin lipopolysaccharide exterior membrane, which could restrict the penetration of the plant extract. A similar study showed that the zone of inhibition of ethanol C. caesia extract on E.coli at 100mg/ml produced zone of inhibition 12mm which was least effective (Kaur et al, 2018). Despite being ineffective against E.coli, the ethanolic extract of this plant showed the maximum zone of inhibition of 22mm at concentration 200mg/ml against S. aureus.…”

Phytochemical and pharmacological properties of Curcuma amada: A Review

“…From Table 1 it was concluded that, chloroform, ethanol, methanol and distilled water extracts were gave good results for terpenoid, cardiac glycosides and gum (Singh and Phucho, 2015) positive results for tannins in ethanol, methanol and distilled water (Singh and Phucho, 2015). (Kaur et al, 2018)revealed the presence of carbohydrates, saponins, glycosides, phytosterols, resins, and lavonoids in rhizome extracts. From Table 1 can conclude the rhizome extract of C.amada exhibited optimistic results for maximum therapeutic chemical constituents.…”

Antimicrobial activity of Curcuma amada extract on Streptococcus mutans—An in vitro study

“…The studies have shown that ethanolic extract of C. amada against various tested microorganisms was most effective compared to other phenolic extracts (acetone, chloroform, ether and carbon tetrachloride) and aqueous extract. [ 6 7 11 ]…”