Comparative assessment of sodium edta and heparin as anticoagulants for the evaluation of haematological parameters in cultured and feral african catfish (Clarias gariepinus)

Adeyemo, Olanike Kudirat; Okwilagwe, O. O.; Ajani, F.

doi:10.14210/bjast.v13n1.p19-24

Cited by 14 publications

(12 citation statements)

References 6 publications

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“…The fish were anaesthetized before blood sampling using 2‐phenoxyethanol (99%; MERCK, Whitehouse Station, NJ, USA) at the concentration of 400 mg⋅l −1 and successively underwent to venipuncture for blood collection. Blood samples were obtained by puncturing the caudal vein using a 20 × 1½‐gauge syringe and collected in microtubes (Miniplast 0.6 ml, LP Italiana Spa, Milano) containing ethylenediaminetetraacetic acid (ratio, 1.26 mg/0.6 ml) as the anticoagulant agent . The time between capture and blood sampling was less than 3 min.…”

Section: Methodscontrasting

confidence: 99%

“…Blood samples were obtained by puncturing the caudal vein using a 20 Â 1½-gauge syringe and collected in microtubes (Miniplast 0.6 ml, LP Italiana Spa, Milano) containing ethylenediaminetetraacetic acid (ratio, 1.26 mg/0.6 ml) as the anticoagulant agent. 25 The time between capture and blood sampling was less than 3 min. All samples were analyzed immediately (T 0 ) by both a manual and automatic method.…”

Section: Methodsmentioning

confidence: 99%

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Effect of storage time on haematological parameters in mullet,Mugil cephalus

Faggio

Casella

Arfuso

et al. 2012

Cell Biochemistry & Function

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The aim of the present study was to assess the effect of storage time at +4 °C on red blood cell count (RBC), haematocrit (Hct), haemoglobin (Hb), white blood cell count (WBC), thrombocyte count (TC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) in mullet (Mugil cephalus) using an automatic method. After blood collection (T0 ), all samples were analyzed using both the manual and automatic method. To test the validation of the automatic method, a paired t-test was applied, and no statistical difference was observed. The samples were successively divided into four different aliquots and stored at +4 °C to assess the haematological parameters using the automatic method. The first aliquot was refrigerated for 6 h, the second one for 24 h, the third one for 48 h and the last one at for 72 h. One-way repeated-measures ANOVA showed a significant effect of storage time (P < 0.05) on Hb, WBC, TC, MCH and MCHC. These results suggest that haematological parameters can be assessed within 6 h from blood collection when samples are stored at +4 °C because long-term storage modifies the results of the analyses. Further studies on these parameters could be still needed in various fish species to validate an appropriate method for haematological analysis useful not only for the evaluation of the health status of animal living in captivity and in aquaculture but also to have reliability environmental haematological biomarkers.

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Section: Methodscontrasting

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Effect of storage time on haematological parameters in mullet,Mugil cephalus

Faggio

Casella

Arfuso

et al. 2012

Cell Biochemistry & Function

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“…Concentrations beyond 2 mg/ml of blood are reported to produce significantly lower values of PVC and higher MCHC, which indicates cell shrinkage and morphological degeneration (Bain et al 2016). An increase in red blood cell volume is thought to be a response to stress due to a variety of factors (Adeyemo et al 2009). Calcium in the nucleated erythrocyte extracellular environment is vital for the maintenance of membrane integrity (Witeska and Wargocka 2011).…”

Section: Discussionmentioning

confidence: 99%

The effect of dipotassium EDTA and lithium heparin on hematologic values of farmed brown trout Salmo trutta (L.) spawners

et al. 2018

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Blood analysis is a very important and powerful diagnostic tool in animal health and welfare control. It is routinely performed in higher vertebrates, for which reference values are well established, but fish hematology still needs further research. Many intrinsic and environmental factors have profound impact on fish hematological values, making determination of reference values difficult. Additionally, fish blood usually requires the addition of an anticoagulant agent, because of short clotting times. The choice of anticoagulant is vital for obtaining reliable blood test values. In the present study, the impact of two common anticoagulants, K 2 EDTA (1.8 mg/ml) and lithium heparin (18 I.U./ml), on hematological values of farmed brown trout Salmo trutta spawners during the spawning season was investigated. Results of basic hematological analysis, such as packed cell volume (PCV), hemoglobin concentration (HGB), red blood cell count (RBC), mean corpuscular hemoglobin (MCH), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and white blood cell count (WBC), were compared between these two compounds. Statistically significant differences were observed in PCV, MCV, and MCHC, whereas HGB, RBC, MCH, and WBC showed no such differences. These results suggest that lithium heparin gives more reliable results, because red blood cells in K 2 EDTA-treated samples have a tendency to swell. It is worth noting that ethylenediaminetetraacetic acid salt did not induce sample hemolysis in the present study.

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“…Cells reaction of various animal species to anticoagulants may be different. Studies on several fish have found that blood cells were unaffected by EDTA, heparin or sodium citrate but both EDTA and citrate reduced white blood cell viability (Walencik and Witeska, 2007;Adeyemo et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…The effects of various types of anticoagulants in hematology were studied in various fish species (Walencik and Witeska, 2007;Witeska and Wargocka, 2011;Ekanem et al, 2012;Adeyemo et al, 2009) but no information's for M. cephalus hematology were found.…”

Section: Introductionmentioning

confidence: 99%

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Faggio¹,

Arfuso²,

Piccione³

et al. 2014

Turk. J. Fish. Aquat. Sci.

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Anticoagulants and storage time's effect on hemogram and differential leukocyte count in 26 Mugil cephalus was investigated. After blood collection, samples were put, in three microtubes containing respectively: ethylenediamine tetraacetic acid (EDTA), heparin, sodium citrate. Analysis were assessed immediately (T0) and 24 h (refrigerated at +4°C) after collection (T24). Statistical analysis showed: significant higher levels of hematocrit (Hct) and hemoglobin (Hb) and lower levels of thrombocytes count (TC) in EDTA (P<0.001) than heparin and sodium citrate treated samples at T0 and T24; white blood cell (WBC) and Lymphocytes values were lower (P<0.05) in sodium citrate than with EDTA and heparin treated samples at T24, while Monocytes levels were higher (P<0.05) in sodium citrate than EDTA and heparin treated samples at T24. Statistical analysis showed also a significant effect of storage time (P<0.01) on TC, Monocytes, Neutrophils, mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) which statistically increase from T0 to T24, while WBC and Lymphocytes statistically decrease from T0 to T24. These finding demonstrate that EDTA showed higher reliability as anticoagulant for this fish and that hematological determinations can be assessed within 24h hours from collection, because storage time modifies analysis results.

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Comparative assessment of sodium edta and heparin as anticoagulants for the evaluation of haematological parameters in cultured and feral african catfish (Clarias gariepinus)

Cited by 14 publications

References 6 publications

Effect of storage time on haematological parameters in mullet,Mugil cephalus

Effect of storage time on haematological parameters in mullet,Mugil cephalus

The effect of dipotassium EDTA and lithium heparin on hematologic values of farmed brown trout Salmo trutta (L.) spawners

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