Comparative assessment of the in vitro toxicity of some functionalized carbon nanotubes and fullerenes

Canape, Carolina; Foillard, Stéphanie; Bonafè, Roberta; Maiocchi, Alessandro; Doris, Eric

doi:10.1039/c5ra11489f

Cited by 17 publications

(13 citation statements)

References 58 publications

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“…Canapè et al have reported the hemocompatibility of the functionalized SWCNTs in human and rat RBCs. 47 Herein, the hemolytic activity of the developed nanoantibiotic was studied. Figure 5B shows the percentage of hemolysis at three concentrations (1, 10, and 20 µg/mL) after 2 and 24 h. It appears that there is no hemolytic activity of all tested concentrations for up to 24 h with a hemolysis percentage <10% as indicated in the literature.…”

mentioning

confidence: 99%

Single-walled carbon nanotubes-ciprofloxacin nanoantibiotic: strategy to improve ciprofloxacin antibacterial activity

Assali¹,

Zaid²,

Abdallah³

et al. 2017

IJN

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As infectious diseases continue to be one of the greatest health challenges worldwide, the demand toward alternative agents is continuously increasing. Recent advancement in nanotechnology has expanded our ability to design and construct nanomaterials to treat bacterial infections. Carbon nanotubes are one among these nanomaterials. Herein, we describe the covalent functionalization of the single-walled carbon nanotubes (SWCNTs) with multiple molecules of ciprofloxacin. The prepared nanoantibiotics were characterized using different techniques, including transmission electron microscopy, Raman spectroscopy, and thermogravimetric analysis. The characterization of the nanoantibiotics confirmed the successful covalent functionalization of the SWCNTs with 55% of functionalization as has been observed by thermogravimetric analysis. The release profile revealed that 90% of the loaded ciprofloxacin was released within 2.5 h at pH 7.4 showing a first-order release profile with R 2 >0.99. Interestingly, the results of the antibacterial activity indicated that the functionalized SWCNTs have significant increase in the antibacterial activity against the three strains of bacteria – by 16-fold for Staphylococcus aureus and Pseudomonas aeruginosa and by 8-fold for Escherichia coli – in comparison to the ciprofloxacin free drug. Moreover, the synthesized nanoantibiotic showed high hemocompatibility and cytocompatibility over a wide concentration range.

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mentioning

confidence: 99%

Single-walled carbon nanotubes-ciprofloxacin nanoantibiotic: strategy to improve ciprofloxacin antibacterial activity

Assali¹,

Zaid²,

Abdallah³

et al. 2017

IJN

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“…Carbon nanotubes in their pristine form or with covalent (e.g., amine, alcohol, carboxylate) or non-covalent (e.g., PEG) surface modifications showed no hemolytic activity in the study of Canapè et al [171]. In vitro analyses showed that SWCNT, and in particular oxidized SWCNT, were not internalized by RBCs even at relatively high doses and did not induce hemolysis or toxic effects on human RBCs [172].…”

Section: Carbon Nanotubesmentioning

confidence: 93%

“…Consumption of C3 and C5 [154] No significant hemolytic activity [171] Pro-coagulant activity in a mouse model, formation of large intravascular aggregates [158]. Amplification of vascular thrombosis in rats (carotid artery) [160] C 2020, 6, 12 of 36 Minimal activation [158].…”

Section: Sw Cnts Pristinementioning

confidence: 99%

Hemocompatibility of Carbon Nanostructures

Fedel

2020

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Carbon nanostructures (CNs), such as carbon nanotubes, fullerenes, carbon dots, nanodiamonds as well as graphene and its derivatives present a tremendous potential for various biomedical applications, ranging from sensing to drug delivery and gene therapy, biomedical imaging and tissue engineering. Since most of these applications encompass blood contact or intravenous injection, hemocompatibility is a critical aspect that must be carefully considered to take advantage of CN exceptional characteristics while allowing their safe use. This review discusses the hemocompatibility of different classes of CNs with the purpose of providing biomaterial scientists with a comprehensive vision of the interactions between CNs and blood components. The various complex mechanisms involved in blood compatibility, including coagulation, hemolysis, as well as the activation of complement, platelets, and leukocytes will be considered. Special attention will be paid to the role of CN size, structure, and surface properties in the formation of the protein corona and in the processes that drive blood response. The aim of this review is to emphasize the importance of hemocompatibility for CNs intended for biomedical applications and to provide some valuable insights for the development of new generation particles with improved performance and safety in the physiological environment.

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“…It was found that whereas one derivative had a negligible effect, the addition of an extra trifluoroacetate group induced a significant, time-dependent alteration of gene expression, mainly in biological processes involving protein synthesis, cell cycle progression and cell adhesion, with the authors suggesting an inhibition effect of the mTOR pathway. In a recent study performed by Canape et al, C 60 fullerenes were covalently functionalized with PEG of various sizes, Full-PEG2000, Full-PEG5000 and Full-PEG10000, and viability was studied on a variety of cell lines 24 h after exposure, evaluating mitochondrial activity, cell membrane integrity and hemolysis [48]. However, all the tested compounds were found to reduce, to some extent, the cellular metabolic activity, only two affected the cell membrane integrity, and none induced hemolysis.…”

Section: Fullerene C 60mentioning

confidence: 99%

Review of In Vitro Toxicity of Nanoparticles and Nanorods—Part 2

Perez¹,

Alsharif²,

Martínez-Banderas³

et al. 2018

Cytotoxicity

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The specific use of engineered nanostructures in biomedical applications has become very attractive, due to their ability to interface and target specific cells and tissues to execute their functions. Additionally, there is continuous progress in research on new nanostructures with unique optical, magnetic, catalytic and electrochemical properties that can be exploited for therapeutic or diagnostic methods. On the other hand, as nanostructures become widely used in many different applications, the unspecific exposure of humans to them is also unavoidable. Therefore, studying and understanding the toxicity of such materials are of increasing importance. Previously published reviews regarding the toxicological effects of nanostructures focus mostly on the cytotoxicity of nanoparticles and their internalization, activated signaling pathways and cellular response. Here, the most recent studies on the invitro cytotoxicity of NPs, nanowires and nanorods for biomedical applications are reviewed and divided into two parts. The first part considers nonmagnetic metallic and magnetic nanostructures, while, the second part covers carbon structures and semiconductors. The factors influencing the toxicity of these nanostructures are elaborated to help elucidate the effects of these nanomaterials on cells, which is a prerequisite for their safe clinical use.

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Comparative assessment of the in vitro toxicity of some functionalized carbon nanotubes and fullerenes

Cited by 17 publications

References 58 publications

Single-walled carbon nanotubes-ciprofloxacin nanoantibiotic: strategy to improve ciprofloxacin antibacterial activity

Single-walled carbon nanotubes-ciprofloxacin nanoantibiotic: strategy to improve ciprofloxacin antibacterial activity

Hemocompatibility of Carbon Nanostructures

Review of In Vitro Toxicity of Nanoparticles and Nanorods—Part 2

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