Comparative bacteriology of juvenile periodontitis

Moore, W. E. C.; Holdeman, Lillian V.; Cato, Elizabeth P.; Smibert, Robert M.; Burmeister, J. A.; Palcanis, Kent G.; Ranney, Richard R.

doi:10.1128/iai.48.2.507-519.1985

Cited by 388 publications

(244 citation statements)

References 26 publications

Supporting

Mentioning

219

Contrasting

Unclassified

Order By: Relevance

“…If these criteria were relaxed, well over 200 microbial taxa have been characterized. Similar cultural findings have been reported by Moore et al (1985). Thus investigators are confronted with far more variables (possible pathogens) than observations (sites that can be conveniently analyzed).…”

Section: Conceptual Problems Probiems Associated With the Microbiotasupporting

confidence: 71%

Difficulties encountered in the search for the etiologic agents of destructive periodontal diseases

Socransky

Smith²,

Dzink³

1987

J Clinic Periodontology

100

View full text Add to dashboard Cite

The present paper outlines some of the difficulties encountered in the search for the etiologic agents of destructive periodontal diseases. These include technical problems such as acquiring an appropriate microbial sample, as well as difficulties in the dispersion, cultivation and identification of isolates in that sample. Many of these difficulties are currently being successfully addressed. A second set of problems is more conceptual in nature. These include difficulties in distinguishing between periodontal diseases and determining the state of activity of periodontal lesions. In addition, complexes of organisms and/or sequences of species may be involved in the progress of lesions. A further problem is encountered in attempting to distinguish overgrowths of opportunistic species from increases in proportions of true pathogens. Finally, it appears likely that different infections occur at the same time in a single oral cavity. The technical and conceptual difficulties eventually filter down to the data analytical step and present numerous problems to the analyst. With all of these difficulties in mind, it is not surprising that the etiologic agents of destructive periodontal diseases are not clearly defined. However, improvement in technological assessments of the microbiota and clinical evaluation of the disease should permit reasoned approaches to be taken. The delineation of the etiologic agents of destructive periodontal diseases will be, of necessity, a multistage iterative process. Etiologic agents will be suggested by predominant cultivable studies and hypotheses concerning subsets of these agents tested using more specific procedures such as selective media, immunofluorescent techniques or DNA probes.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

Section: Conceptual Problems Probiems Associated With the Microbiotasupporting

confidence: 71%

Difficulties encountered in the search for the etiologic agents of destructive periodontal diseases

Socransky

Smith²,

Dzink³

1987

J Clinic Periodontology

100

View full text Add to dashboard Cite

show abstract

“…They aiso recommended the avoidance of excessively small or large samples. The use of curette sampling was also supported by the findings of Moore et al (!982a), who showed that sealer sampling provided higher numbers of Capnocytophaga and Etibacteriian species than other methods, and counts similar to samples obtained with paper points (Moore et al 1985). The problems of possible mechanical disturbance of the plaque sample by bleeding or contamination by more coronally located subgingival plaque during insertion and withdrawal of the curette was noted in the present study.…”

Section: Discussionsupporting

confidence: 66%

“…Evian et al 1982. Magnusson et al 1985, Moore et al 1985, and Pihlstrom et al 1985. In spite of the attempts by Williams et al (1976), Darwish et al (1978).…”

Section: Discussionmentioning

confidence: 99%

False results associated with darkground microscopy of subgingival plaque

Omar

Newman

1986

J Clinic Periodontology

View full text Add to dashboard Cite

Abstract. This study considers false results which may arise due to problems in the preparation or examination of specimens for darkground microscopy of subgingival plaque. Subgingival plaque samples obtained with a sterile curette were placed in 0.1–0.3 ml sterile full or 1/4 strength Ringer's solution: 0.85% saline, 1% gelatin in 0.85% saline, formal saline or pyrogen‐free water for injection. Test slides were prepared from the original dispersion, and control slides from the corresponding sterile solution. Optimal dispersion solution, syringe dispersion frequency and the effect on motility of delay in processing samples were tested. Slides were also prepared from dispersions of 11 representative subgingival “periodontopathic” organisms. Problems in sampling included variability in counts between sites with comparable pocket depths, contamination of the sample and reduction of the sample volume after scaling. Problems in dispersion included contamination, uneven distribution of the different morphotypes and destruction of delicate organisms. Problems in slide preparation included slide contamination, limitation in the number of samples that can be assessed by one examiner at a given time without loss of activity of motile cells, and preparation of a cell monolayer. Problems in identification and counting included confusion of Brownian movements with motility, coccoid particles with cocci, spirochetes with campylobacter, flagella with flagella‐like structures, size of cocci, counting of fragmented spirochetes and non‐motile flagellated organisms and motile cells, and also bias in counting. Problems in morpbotype grouping included the observation that many (10 of the 11 representative) periodontitis‐related organisms were in the non‐motile groups and not all cells of the motile species (Campylobacter, Capnocytophaga) showed motility. The results indicate that each stage of subgingival plaque darkground microscopy, sampling, dispersion, slide preparation, counting, morphotype grouping and interpretation may lead to false results if not representative or reproducible. Procedures are suggested for the minimisation of problems in the preparation and examination of subgingival plaque specimens for darkground microscopy.

show abstract

“…The tongue biofilm coating is considered to be the most important source of VSCs, simply because it harbours the highest population of microbes (Greenman et al 2005). To date, the exact number of different species resident within the oral cavity is unknown, although several attempts have estimated numbers ranging from; 500 species (Moore et al 1985, Paster et al 2001, to 600 species (Kazor et al 2003), and up to 700 species (Aas et al 2005). Although the actual exact number will probably remain unknown due to the high diversity of resident oral flora, most estimates propose that over 50% of the microorganisms within the oral cavity are uncultivable, and their presence has only been identified by molecular rDNA testing (Aas et al 2005, Paster et al 2001.…”

Section: Oral Disease and Malodourmentioning

confidence: 99%

Microbial volatile compounds in health and disease conditions

Thorn¹,

Greenman²

2012

J. Breath Res.

113

View full text Add to dashboard Cite

Microbial cultures and/or microbial associated diseases often have a characteristic smell. Volatile organic compounds (VOCs) are produced by all microorganisms as part of their normal metabolism. The types and classes of VOC produced is wide, including fatty acids and their derivatives (e.g. hydrocarbons, aliphatic alcohols and ketones), aromatic compounds, nitrogen containing compounds, and volatile sulfur compounds. A diversity of ecological niches exist in the human body which can support a polymicrobial community, with the exact VOC profile of a given anatomical site being dependent on that produced by both the host component and the microbial species present. The detection of VOCs is of interest to various disciplines, hence numerous analytical approaches have been developed to accurately characterize and measure VOCs in the laboratory, often from patient derived samples. Using these technological advancements it is evident that VOCs are indicative of both health and disease states. Many of these techniques are still largely confined to the research laboratory, but it is envisaged that in future bedside 'VOC profiling' will enable rapid characterization of microbial associated disease, providing vital information to healthcare practitioners.

show abstract

Comparative bacteriology of juvenile periodontitis

Cited by 388 publications

References 26 publications

Difficulties encountered in the search for the etiologic agents of destructive periodontal diseases

Difficulties encountered in the search for the etiologic agents of destructive periodontal diseases

False results associated with darkground microscopy of subgingival plaque

Microbial volatile compounds in health and disease conditions

Contact Info

Product

Resources

About