Comparative Biochemistry of the Oxidative Burst Produced by Rose and French Bean Cells Reveals Two Distinct Mechanisms1

Gp, Bolwell; Dr, Davies; Gerrish, Chris; Ck, Auh; Murphy, TM

doi:10.1104/pp.116.4.1379

Cited by 253 publications

(188 citation statements)

References 36 publications

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“…However, different plant species can place emphasis on different mechanisms for the production of active oxygen species in response to elicitation (Bolwell et al, 1998;Papadakis and Roubelakis-Angelakis, 1999). Our results indicate that increases in either GSH or salicylic acid are insufficient for the propagation of an oxidative burst in grapevine callus cultures.…”

Section: Discussionmentioning

confidence: 63%

“…Similarly low concentrations (0.1-10 m) of DPI effectively inhibit the elicited formation of H 2 O 2 from membrane-bound plant NADH-oxidases (Levine et al, 1994). Substantially higher concentrations (50-240 m) of DPI are required to inhibit H 2 O 2 production from NADH oxidase-peroxidase (Bolwell et al, 1998;Frahry and Schopfer, 1998), which can be important for the development of the oxidative burst in some plant species (Bolwell et al, 1995). The sensitivity of grapevine callus to DPI therefore suggests that the elicited deposition of GvP1 is driven by an oxidative burst from membrane-bound NADH oxidase.…”

Section: Discussionmentioning

confidence: 99%

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Rapid Deposition of Extensin during the Elicitation of Grapevine Callus Cultures Is Specifically Catalyzed by a 40-Kilodalton Peroxidase

et al. 2001

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Elicitation or peroxide stimulation of grape (Vitis vinifera L. cv Touriga) vine callus cultures results in the rapid and selective in situ insolubilization of an abundant and ionically bound cell wall protein-denominated GvP1. Surface-enhanced laser desorption/ionization/time of flight-mass spectrometry analysis, the amino acid composition, and the N-terminal sequence of purified GvP1 identified it as an 89.9-kD extensin. Analysis of cell walls following the in situ insolubilization of GvP1 indicates large and specific increases in the major amino acids of GvP1 as compared with the amino acids present in salt-eluted cell walls. We calculate that following deposition, covalently bound GvP1 contributes up to 4% to 5% of the cell wall dry weight. The deposition of GvP1 in situ requires peroxide and endogenous peroxidase activity. Isoelectric focusing of saline eluates of callus revealed only a few basic peroxidases that were all isolated or purified to electrophoretic homogeneity. In vitro and in situ assays of extensin cross-linking activity using GvP1 and peroxidases showed that a 40-kD peroxidase cross-linked GvP1 within minutes, whereas other grapevine peroxidases had no significant activity with GvP1. Internal peptide sequences indicated this extensin peroxidase (EP) is a member of the class III peroxidases. We conclude that we have identified and purified an EP from grapevine callus that is responsible for the catalysis of GvP1 deposition in situ during elicitation. Our results suggest that GvP1 and this EP play an important combined role in grapevine cell wall defense.

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Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Rapid Deposition of Extensin during the Elicitation of Grapevine Callus Cultures Is Specifically Catalyzed by a 40-Kilodalton Peroxidase

et al. 2001

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“…The GSH functions as an important antioxidant in both enzymatic and nonenzymatic ROS scavenging reactions (Foyer and Halliwell, 1976;Noctor and Foyer, 1998), and both Cys and N-aC are commonly used as antioxidants to scavenge ROS and protect against oxidative damage (Bolwell et al, 1998). In support of the importance of these thiols in such protective mechanisms, several transgenic plants with elevated levels of GSH have been shown to be particularly resistant to oxidative stress (Foyer et al, 1995;Wellburn et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Increased Cysteine Biosynthesis Capacity of Transgenic Tobacco Overexpressing an O-Acetylserine(thiol) Lyase Modifies Plant Responses to Oxidative Stress

et al. 2001

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O-Acetylserine(thiol) lyase (OASTL), a key enzyme of plant sulfur metabolism, catalyzes the formation of Cys from sulfide and O-acetylserine. The biosynthesis of Cys is regarded as the exclusive function of sulfur reduction in plants, and a key limiting step in the production of glutathione (GSH), a thiol implicated in various cellular functions, including sulfur transport, gene expression, scavenging of reactive oxygen species (ROS), and resistance to biotic and abiotic stresses. To examine whether an increased capacity for cysteine (Cys) biosynthesis alters cellular responses to such stresses, we studied the differential changes in thiol levels and ROS scavenging of transgenic tobacco (Nicotiana tabacum) plants expressing the wheat (Triticum aestivum) OASTL gene, cys1, to SO 2 and to the ROS generator, methyl viologen. Intracellular Cys and GSH contents were generally higher in cys1 transgenics than in controls under normal growth conditions, but became especially elevated in transgenic plants after SO 2 exposure. An examination of differences in the ROS scavenging system of the transgenic plants also demonstrated the specific accumulation of Cu/Zn superoxide dismutase transcripts, known to be induced by Cys or GSH, and elevated cellular superoxide dismutase activities. The transgenic plants accordingly showed dramatic reductions in the extent of both foliar and photooxidative damage in response to acute SO 2 , as well as reduced levels of chlorosis and membrane damage following methyl viologen treatment. Overall, our results imply that OASTL plays a pivotal role in the synthesis of Cys and GSH that are required for regulation of plant responses to oxidative stress.

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“…27,28 We have recently proposed a likely role for free Fe 2C in reduction of ferric HRP into ferrous intermediate and Compound III which may further produces O 2 ¡ , especially under alkaline condition possibly contributing to the plant defense mechanism. 18 The present work confirmed the similar behavior of SBP in response to Fe 2C under alkaline condition.…”

Section: E1010917-4mentioning

confidence: 99%

Hydrogen peroxide-independent generation of superoxide catalyzed by soybean peroxidase in response to ferrous ion

Kimura

Kawano

2015

Plant Signaling & Behavior

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Comparative Biochemistry of the Oxidative Burst Produced by Rose and French Bean Cells Reveals Two Distinct Mechanisms1

Cited by 253 publications

References 36 publications

Rapid Deposition of Extensin during the Elicitation of Grapevine Callus Cultures Is Specifically Catalyzed by a 40-Kilodalton Peroxidase

Rapid Deposition of Extensin during the Elicitation of Grapevine Callus Cultures Is Specifically Catalyzed by a 40-Kilodalton Peroxidase

Increased Cysteine Biosynthesis Capacity of Transgenic Tobacco Overexpressing an O-Acetylserine(thiol) Lyase Modifies Plant Responses to Oxidative Stress

Hydrogen peroxide-independent generation of superoxide catalyzed by soybean peroxidase in response to ferrous ion

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