Comparative Biosorption Capacity of Copper and Chromium by Bacillus Cereus

A, Saravana Raj; P, Vinosh Muthukumar; Bharathiraja, B.; Priya, M.

doi:10.14419/ijet.v7i3.34.19355

Cited by 3 publications

(2 citation statements)

References 5 publications

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“…In the pilot scale study, B. altitudinis MT422188 was able to remove 73 mg/l and 82 mg/l at 4 and 8 day intervals, which demonstrated its biosorption ability to be efficient in removing Cu ( Figure 4 ) . B. cereus has been reported to effectively remove more than 40 and 50% of Cu from industrial effluents ( Raj et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Harnessing the Potential of Bacillus altitudinis MT422188 for Copper Bioremediation

et al. 2022

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The contamination of heavy metals is a cause of environmental concern across the globe, as their increasing levels can pose a significant risk to our natural ecosystems and public health. The present study was aimed to evaluate the ability of a copper (Cu)-resistant bacterium, characterized as Bacillus altitudinis MT422188, to remove Cu from contaminated industrial wastewater. Optimum growth was observed at 37°C, pH 7, and 1 mm phosphate, respectively. Effective concentration 50 (EC50), minimum inhibitory concentration (MIC), and cross-heavy metal resistance pattern were observed at 5.56 mm, 20 mm, and Ni > Zn > Cr > Pb > Ag > Hg, respectively. Biosorption of Cu by live and dead bacterial cells in its presence and inhibitors 1 and 2 (DNP and DCCD) was suggestive of an ATP-independent efflux system. B. altitudinis MT422188 was also able to remove 73 mg/l and 82 mg/l of Cu at 4th and 8th day intervals from wastewater, respectively. The presence of Cu resulted in increased GR (0.004 ± 0.002 Ug−1FW), SOD (0.160 ± 0.005 Ug−1FW), and POX (0.061 ± 0.004 Ug−1FW) activity. Positive motility (swimming, swarming, twitching) and chemotactic behavior demonstrated Cu as a chemoattractant for the cells. Metallothionein (MT) expression in the presence of Cu was also observed by SDS-PAGE. Adsorption isotherm and pseudo-kinetic-order studies suggested Cu biosorption to follow Freundlich isotherm as well as second-order kinetic model, respectively. Thermodynamic parameters such as Gibbs free energy (∆G°), change in enthalpy (∆H° = 10.431 kJ/mol), and entropy (∆S° = 0.0006 kJ/mol/K) depicted the biosorption process to a feasible, endothermic reaction. Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and Energy-Dispersive X-Ray Spectroscopy (EDX) analyses revealed the physiochemical and morphological changes in the bacterial cell after biosorption, indicating interaction of Cu ions with its functional groups. Therefore, these features suggest the potentially effective role of B. altitudinis MT422188 in Cu bioremediation.

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Section: Discussionmentioning

confidence: 99%

Harnessing the Potential of Bacillus altitudinis MT422188 for Copper Bioremediation

et al. 2022

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“…Like many other heavy metal ions, Cu is considered to be essential for Bacillus subtilis , while concentrations exceeding normal amounts can be toxic for the cells. Species like B. thuringiensis , B. cereus , B. licheniformis , and B. sphaericus are also involved in the removal of Cu, when their concentrations exceed the required limit [ 117 , 128 , 129 , 130 , 131 , 132 , 133 , 255 , 256 ] ( Table 1 ). In correlation with other bacterial species, Cu in the cytosol is regulated by CueR [ 257 ].…”

Section: Bacillus Species and Heavy Metalsmentioning

confidence: 99%

Unraveling the Underlying Heavy Metal Detoxification Mechanisms of Bacillus Species

2021

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The rise of anthropogenic activities has resulted in the increasing release of various contaminants into the environment, jeopardizing fragile ecosystems in the process. Heavy metals are one of the major pollutants that contribute to the escalating problem of environmental pollution, being primarily introduced in sensitive ecological habitats through industrial effluents, wastewater, as well as sewage of various industries. Where heavy metals like zinc, copper, manganese, and nickel serve key roles in regulating different biological processes in living systems, many heavy metals can be toxic even at low concentrations, such as mercury, arsenic, cadmium, chromium, and lead, and can accumulate in intricate food chains resulting in health concerns. Over the years, many physical and chemical methods of heavy metal removal have essentially been investigated, but their disadvantages like the generation of chemical waste, complex downstream processing, and the uneconomical cost of both methods, have rendered them inefficient,. Since then, microbial bioremediation, particularly the use of bacteria, has gained attention due to the feasibility and efficiency of using them in removing heavy metals from contaminated environments. Bacteria have several methods of processing heavy metals through general resistance mechanisms, biosorption, adsorption, and efflux mechanisms. Bacillus spp. are model Gram-positive bacteria that have been studied extensively for their biosorption abilities and molecular mechanisms that enable their survival as well as their ability to remove and detoxify heavy metals. This review aims to highlight the molecular methods of Bacillus spp. in removing various heavy metals ions from contaminated environments.

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Bacterial Remediation and Prospects for Its Utilization (Review)

Sizencov,

Sal'nikova

2024

Ekosistemy

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The escalating technogenic burden on the environment has adverse effects on ecological systems at various levels. Heavy metals significantly contribute to environmental contamination due to their strong biological impact and cumulative properties. The search for new methodological approaches to mitigate the consequences of technogenic pollution induced by heavy metals is an urgent task for ensuring the environmental sustainability of the region. Physical and chemical remediation techniques have several drawbacks, such as high costs and incomplete removal, which can lead to secondary contamination. Bacterial remediation is a highly efficient method that ensures a reduction in the level of human-induced pressure on the ecological system: bacteria – water – soil – plant – animal – human. The article examines meta-analytical data on the mechanisms of interaction between bacterial cells and metals, as well as methods of soil bioremediation and assessment of the sorption characteristics of microorganisms from the genus Bacillus sp. The results of empirical studies presented in the review demonstrate significant bioremediation potential of representatives of this group towards essential and xenobiotic elements from the group of heavy metals. Thus, the level of mercury sorption from substrates can reach up to 96.40 % of the applied concentration, lead – up to 99.5 %, cadmium – 98.3 %, arsenic – 98.3 %, nickel – 99.2 %, chromium – 95.0 %, copper – 91.8 % and zinc – 87.0 %, respectively. The research focuses on developing alternative methods that are not only highly efficient but also environmentally friendly for remediating areas affected by human-induced stress, by utilizing bacterial cell populations. The majority of the analyzed works studies use indigenous strains the tolerance to metals and sorption capacity of which are determined by their selection characteristics under conditions of excessive cationic load of metals on their environment.

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Comparative Biosorption Capacity of Copper and Chromium by Bacillus Cereus

Cited by 3 publications

References 5 publications

Harnessing the Potential of Bacillus altitudinis MT422188 for Copper Bioremediation

Harnessing the Potential of Bacillus altitudinis MT422188 for Copper Bioremediation

Unraveling the Underlying Heavy Metal Detoxification Mechanisms of Bacillus Species

Bacterial Remediation and Prospects for Its Utilization (Review)

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