The purpose of the study was to determine the milk productivity and chemical composition of the milk of mares of the Kazakh breed of the jabe type (KJ) and the Novoaltay-Kazakh crossbreeds of the 1st generation (NA x KJ) in the conditions of herd keeping. To determine the milk productivity of mares, 2 experimental groups were formed with a total of 30 mares, 15 individuals were selected in each group. The studied animals were formed according to the principle of pairs of analogues in the context of full-age groups from 5 to 11 years. Kazakh horses of the jabe type in the group of experimental mares in milk have an average live weight of 433.5 kg, and are significantly inferior to the Novoaltay-Kazakh crosses (515.3 kg). The body measurements were 142.0-149.0-174.9-18.2 cm in Kazakh horses of the jabe type, and 150.1-157.1-186.7-20.02 cm, respectively, in Novoaltay-Kazakh crossbreeds. According to the results of studies, the milkiness of mares of the Novoaltay-Kazakh crosses for 105 days of lactation averaged 1932 liters or higher by 25.2% (487.2 liters) than the milkiness of mares of the Kazakh breed of the jabe type. As a result of studies on the composition of milk of mares of different genotypes, it was found that in mares of Kazakh jabe, on average, protein and fat indicators compared to Novoaltay-Kazakh crossbreeds were higher by 0.07% and 0.05%. In the course of determining the live weight of mares among the experimental groups, it was found that the Novoaltay-Kazakh crossbreeds, on average, had a higher live weight of 515.3 kg or higher compared to the Kazakh jabe by 81.8 kg (15.8%). Thus, the studies have proved the prospects of using stallions of the Novoaltay breed to increase the milkiness of local Kazakh horses of the jabe type in herd and pasture conditions.