Comparative characteristics of endothelial-like cells derived from human adipose mesenchymal stem cells and umbilical cord blood-derived endothelial cells

Abstract

Adult peripheral blood contains a limited number of endothelial progenitor cells that can be isolated for treatment of ischemic diseases. The adipose tissue became an interesting source of stem cells for regenerative medicine. This study aimed to investigate the phenotype of cells obtained by culturing adipose-derived mesenchymal stem cells (ad-MSCs) in the presence of endothelial growth supplements compared to endothelial cells obtained from umbilical cord blood (UCB). Passage 3 ad-MSCs and mononuclear layer from UCB were cultured in presence of endothelial growth media for 3 weeks followed by their characterization by flow cytometry and polymerase chain reaction. After culture in endothelial inductive media, ad-MSCs expressed endothelial genes and some endothelial marker proteins as CD31 and CD34, respectively. Adipose tissue could be a reliable source for easy obtaining, expanding and differentiating MSCs into endothelial-like cells for autologous cell-based therapy.

RETRACTED ARTICLE: Small Diameter Blood Vessels Bioengineered From Human Adipose-derived Stem Cells

“…Another promising cell source is adult mesenchymal stem cells 25 . The hASCs derived from mesoderm differentiate into multiple lineages 17 18 19 20 . Cao et al 26 demonstrated that hASCs showed morphology and function similar to that of endothelial cells when cultured in media containing VEGF and bFGF.…”

“…Recently, hASCs have demonstrated the potential for differentiation into multiple cell lineages, including osteocytes, chondrocytes, smooth muscle cells and endothelial cells 7 8 9 10 11 12 13 14 15 16 17 18 19 20 . In addition, hASCs are easier to obtain and exhibit lower donor-site morbidity.…”

Bone marrow stromal cells transplantation combined with ultrashortwave therapy promotes functional recovery on spinal cord injury in rats

“…Various studies that have used BMSCs transplantation to treat SCI have demonstrated that they can differentiate into functional neurons or glias, while secreting neurotrophic factors that modulate local inflammation, thereby creating a microenvironment that facilitates nerve regeneration and is conducive to functional recovery (Cui et al, ; Kim et al, ; Liu et al, ; Roh et al, ; Jarocha et al, ). Besides the bone marrow, BMSCs can be obtained from umbilical cord and peripheral blood and fat tissue, and the source of BMSCs dictate their differentiation potential (Gaafar et al, ; Pang et al, ; Pham et al, ). Implantation of BMSCs‐derived cells has been reported to elicit de novo neurogenesis and functional recovery in a nonhuman primate model of spinal cord injury (Deng et al, ).…”

Non-Genetic Direct Reprogramming and Biomimetic Platforms in a Preliminary Study for Adipose-Derived Stem Cells into Corneal Endothelia-Like Cells

“…Primary ADSCs also partly expressed CD34 and CD105, which showed that these ADSCs were comprised of heterogeneous cell populations. CD34 was quiescence stem cell and endothelial cell marker [40] . Usually, CD105 expression was relatively low on the freshly isolated ADSCs.…”