2007
DOI: 10.1016/j.foodchem.2006.02.035
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Comparative characterization of monophenolase and diphenolase activities from a wild edible mushroom (Macrolepiota mastoidea)

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Cited by 23 publications
(15 citation statements)
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“…Interestingly, we found a very strong correlation (r = 0.85; P = 0.07) between copper content of fruit and PPO activity (Table 6). According to Aydemir (2004), Cu ++ and Fe +++ ions at 1 mM caused the activation of PPO, but at 10 mM concentration, both Cu ++ and Fe +++ ions acted as poor inhibitors of PPO, whereas, Colak et al (2007) and Kolcuoglu et al (2007) have recently found that Cu ++ at 1 mM concentration was sufficient to inhibit PPO activity. As well, the reported literature on the effects of different metal ions on the PPO activity is conflicting.…”
Section: Resultsmentioning
confidence: 99%
“…Interestingly, we found a very strong correlation (r = 0.85; P = 0.07) between copper content of fruit and PPO activity (Table 6). According to Aydemir (2004), Cu ++ and Fe +++ ions at 1 mM caused the activation of PPO, but at 10 mM concentration, both Cu ++ and Fe +++ ions acted as poor inhibitors of PPO, whereas, Colak et al (2007) and Kolcuoglu et al (2007) have recently found that Cu ++ at 1 mM concentration was sufficient to inhibit PPO activity. As well, the reported literature on the effects of different metal ions on the PPO activity is conflicting.…”
Section: Resultsmentioning
confidence: 99%
“…Antioxidant activity in acidified by 0.5% HCl methanol extracts (40 mg sample/1 ml extract) was assayed for scavenging capacity of the DPPH radical (Pekkarinen et al, 1999) and ABTS cation radical (Re et al, 1999). Total polyphenoloxidases activity (PPO) was determined according to Cano et al (1997), peroxidase (PER) and catalase (CAT) activity according to Bergmeyer (1974), monophenolase (MON) and diphenolase (DIP) according to Kolcuoglu et al (2007). Analyses were carried out in four replications for each sample.…”
Section: Methodsmentioning
confidence: 99%
“…The antioxidant enzymes scavenged O 2 -and H 2 O 2 , which peroxidation and accelerated cell senescence. According toKolcuoglu et al (2007), monophenolase in mushrooms can be inhibited by thiourea and ascorbic acid, and diphenolase by sodium metabisulphite and ascorbic acid.The activity of catalase (CAT), total polyphenol oxidase (PPO), monophenolase (MON) and diphenolase (DIP) in fresh A. bisporus mushrooms was determined. In the case of peroxidase activity, zero or trace levels were detected in both fresh and frozen mushrooms (Tab.…”
mentioning
confidence: 99%
“…For example, for commercial mushroom PPO, the loss of monophenolase activity was observed at pH 3.0, 4.0 and 5.0, while the enzyme is very stable at pH 6.0 [18]. The same phenomenon was observed at pH 3.0 for the monophenolase activity of a wild edible mushroom (Macrolepiota mastoidea), while the enzyme showed highest stability at pH 4.0 [38]. The loss of labile monophenolase activity was also frequently observed during purification of PPO by different workers [2,39,40].…”
Section: Ph Stabilitymentioning
confidence: 65%