Comparative characterization of two cyanobacteria strains of the order Spirulinales isolated from the Baltic Sea - polyphasic approach in practice

Szubert, Karolina; Toruńska-Sitarz, Anna; Stoń-Egiert, Joanna; Wiglusz, Magda; Mazur‐Marzec, Hanna

doi:10.1016/j.algal.2020.102170

Cited by 3 publications

(1 citation statement)

References 76 publications

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“…The air temperature while sampling was 32°C. Aliquots of 0.1 mL of collected soil sample dilutions (10 3 -10 5 ) were streaked into CT solid medium (Andersen 2005) following the slightly modification using NaNO 3 (2,260 µmol L -1 ) as a nitrogen source until a single cyanobacterial colony ap-relationships (Řeháková et al 2007, Hašler et al 2012, Shalygin et al 2017, McGregor and Sendall 2021, Szubert et al 2021. Recently, the polyphasic approach combining molecular, morphological, ecological and other data (ultrastructure, biochemical analysis) has been used for cyanobacterial classification to assist the recognition of new taxa (Komárek et al 2014, Komárek and Johansen 2015, Komárek 2016.…”

Section: Isolation and Cultivationmentioning

confidence: 99%

Amazonocrinis thailandica sp. nov. (Nostocales, Cyanobacteria), a novel species of the previously monotypic Amazonocrinis genus from Thailand

Tawong¹,

Pongcharoen²,

Pongpadung³

et al. 2022

Algae

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Cyanobacteria are distributed worldwide, and many new cyanobacterial species are discovered in tropical region. TheNostoc-like genus Amazonocrinis has been separated from the genus Nostoc based on polyphasic methods. However,species diversity within this genus remains poorly understood systematically because only one species (Amazonocrinisnigriterrae) has been described. In this study, two novel strains (NUACC02 and NUACC03) were isolated from moist ricefield soil in Thailand. These two strains were characterized using a polyphasic approach, based on morphology, 16S rRNAphylogenetic analysis, internal transcribed spacer secondary structure and ecology. Phylogenetic analyses based on 16SrRNA gene sequences confirmed that the two novel strains formed a monophyletic clade related to the genus Amazonocrinisand were distant from the type species A. nigriterrae. The 16S rRNA gene sequence similarity (<98.1%) betweennovel strains and all other closely related taxa including the Amazonocrinis members exceeded the cutoff for speciesdelimitation in bacteriology, reinforcing the presence of a new Amazonocrinis species. Furthermore, the novel strainspossessed unique phenotypic characteristics such as the presence of the sheath, necridia-like cells, larger cell dimensionand akinete cell arrangement in long-chains and the singularity of D1–D1′, Box-B, V2, and V3 secondary structures thatdistinguished them from other Amazonocrinis members. Considering all the results, we described our two strains as Amazonocrinisthailandica sp. nov. in accordance with the International Code of Nomenclature for Algae, Fungi and Plants.

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Section: Isolation and Cultivationmentioning

confidence: 99%

Amazonocrinis thailandica sp. nov. (Nostocales, Cyanobacteria), a novel species of the previously monotypic Amazonocrinis genus from Thailand

Tawong¹,

Pongcharoen²,

Pongpadung³

et al. 2022

Algae

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Biology and composition of microalgae and cyanobacteria

Rodríguez-Zúñiga,

Méndez-Zavala,

Solís-Quiroz

et al. 2024

Sustainable Industrial Processes Based on Microalgae

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Biodiversity of microorganisms in the Baltic Sea: the power of novel methods in the identification of marine microbes

Mazur-Marzec,

Andersson,

Błaszczyk

et al. 2024

FEMS Microbiology Reviews

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Until recently, the data on the diversity of the entire microbial community from the Baltic Sea were relatively rare and very scarce. However, modern molecular methods have provided new insights into this field with interesting results. They can be summarized as follows. (i) Although low salinity causes a reduction in the biodiversity of multicellular species relative to the populations of the North-East Atlantic, no such reduction occurs in bacterial diversity. (ii) Among cyanobacteria, the picocyanobacterial group dominates when considering gene abundance, while filamentous cyanobacteria dominate in means of biomass. (iii) The diversity of diatoms and dinoflagellates is significantly larger than described a few decades ago; however, molecular studies on these groups are still scarce. (iv) Knowledge gaps in other protistan communities are evident. (v) Salinity is the main limiting parameter of pelagic fungal community composition, while the benthic fungal diversity is shaped by water depth, salinity, and sediment C and N availability. (vi) Bacteriophages are the predominant group of viruses, while among viruses infecting eukaryotic hosts, Phycodnaviridae are the most abundant; the Baltic Sea virome is contaminated with viruses originating from urban and/or industrial habitats. These features make the Baltic Sea microbiome specific and unique among other marine environments.

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Comparative characterization of two cyanobacteria strains of the order Spirulinales isolated from the Baltic Sea - polyphasic approach in practice

Cited by 3 publications

References 76 publications

Amazonocrinis thailandica sp. nov. (Nostocales, Cyanobacteria), a novel species of the previously monotypic Amazonocrinis genus from Thailand

Amazonocrinis thailandica sp. nov. (Nostocales, Cyanobacteria), a novel species of the previously monotypic Amazonocrinis genus from Thailand

Biology and composition of microalgae and cyanobacteria

Biodiversity of microorganisms in the Baltic Sea: the power of novel methods in the identification of marine microbes

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