Comparative Clinical Observation of Arthroscopic Microfracture in the Presence and Absence of a Stromal Vascular Fraction Injection for Osteoarthritis

Nguyen, Phu; Tran, Tung Dang-Xuan; Nguyen, Huynh Ton-Ngoc; Vu, Hieu Trung; Le, Phuong Thi-Bich; Vu, Ngoc Bich; Phan, Ngoc Kim; Pham, Phuc Van

doi:10.5966/sctm.2016-0023

Cited by 99 publications

(174 citation statements)

References 81 publications

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“…Adipose tissues from patients were collected according to a previously published study (Nguyen et al, 2016). Briefly, patients were given local anesthesia with 2-3 mL of marcaine (5 g/L); the lower abdomen area was also anesthetized.…”

Section: Adipose Tissue Aspirationmentioning

confidence: 99%

Expanded autologous adipose derived stem cell transplantation for type 2 diabetes mellitus

Pham

et al. 2016

Biomed Res Ther

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Introduction: Type 2 diabetes mellitus (T2D) is the most common form of diabetes mellitus, accounting for 90% of diabetes mellitus in patients. At the present time, although T2D can be treated by various drugs and therapies using insulin replacement, reports have shown that complications including microvascular, macrovascular complications and therapy resistance can occur in patients on long term treatment. Stem cell therapy is regarded as a promising therapy for diabetes mellitus, including T2D. The aim of this study was to evaluate the safety and therapeutic effect of expanded autologous adipose derived stem cell (ADSC) transplantation for T2D treatment; the pilot study included 3 patients who were followed for 3 months. Methods: The ADSCs were isolated from stromal vascular fractions, harvested from the belly of the patient,and expanded for 21 days per previously published studies. Before transplantation, ADSCs were evaluated for endotoxin, mycoplasma contamination, and karyotype.All patients were transfused with ADSCs at 1-2x10 6 cells/kg of body weight.Patients were evaluated for criteria related to transplantation safety and therapeutic effects; these included fever, blood glucose level before transplantation of ADSCs, and blood glucose level after transplantation (at 1, 2 and 3 months). Results: The results showed that all samples of ADSCs exhibited the MSC phenotype with stable karyotype (2n=46), there was no contamination of mycoplasma, and endotoxin levels were low (<0.25 EU/mL). No adverse effects were detected after 3 months of transplantation. Decreases of blood glucose levels were recorded in all patients. Conclusion: The findings from this initial study show that expanded autologous ADSCs may be a promising treatment for T2D.

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Section: Adipose Tissue Aspirationmentioning

confidence: 99%

Expanded autologous adipose derived stem cell transplantation for type 2 diabetes mellitus

Pham

et al. 2016

Biomed Res Ther

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“…Patients have experienced improvements in pain, function, mobility, and overall quality of life on various clinical questionnaires after SVF and ASC administration into the affected joints (Fig. H) . Clinical improvement persisted in these studies for several months to more than two years.…”

Section: Resultsmentioning

confidence: 80%

“…Various studies have been conducted in which human patients with osteoarthritis of the knee received an intra‐articular injection with autologous SVF and ASC. Many of the studies specifically recruited patients with osteoarthritis which was refractory to oral medications, physical therapy, autologous cartilage transplantation, and hyaluronic acid injection . Patients have experienced improvements in pain, function, mobility, and overall quality of life on various clinical questionnaires after SVF and ASC administration into the affected joints (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Clinical improvement persisted in these studies for several months to more than two years. Additionally, MRI and arthroscopy have demonstrated improvement or maintenance of cartilage status along the same time period after SVF or ASC administration . Preclinical studies suggest that these effects of ASCs may be due to ASC‐mediated reduction of pro‐inflammatory cytokines and chemokines, apoptosis of chondrocytes, hypertrophic and fibrotic chondrocyte phenotypes, and collagenases .…”

Section: Resultsmentioning

confidence: 99%

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Concise Review: Using Fat to Fight Disease: A Systematic Review of Nonhomologous Adipose-Derived Stromal/Stem Cell Therapies

et al. 2018

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The objective of this Review is to describe the safety and efficacy of adipose stem/stromal cells (ASC) and stromal vascular fraction (SVF) in treating common diseases and the next steps in research that must occur prior to clinical use. Pubmed, Ovid Medline, Embase, Web of Science, and the Cochrane Library were searched for articles about use of SVF or ASC for disease therapy published between 2012 and 2017. One meta-analysis, 2 randomized controlled trials, and 16 case series were included, representing 844 human patients. Sixty-nine studies were performed in preclinical models of disease. ASCs improved symptoms, fistula healing, remission, and recurrence rates in severe cases of inflammatory bowel disease. In osteoarthritis, ASC and SVF improved symptom-related, functional, radiographic, and histological scores. ASC and SVF were also shown to improve clinical outcomes in ischemic stroke, multiple sclerosis, myocardial ischemia, chronic obstructive pulmonary disease, idiopathic pulmonary fibrosis, chronic liver failure, glioblastoma, acute kidney injury, and chronic skin wounds. These effects were primarily paracrine in nature and mediated through reduction of inflammation and promotion of tissue repair. In the majority of human studies, autologous ASC and SVF from liposuction procedures were used, minimizing the risk to recipients. Very few serious, treatment-related adverse events were reported. The main adverse event was postprocedural pain. SVF and ASC are promising therapies for a variety of human diseases, particularly for patients with severe cases refractory to current medical treatments. Further randomized controlled trials must be performed to elaborate potential safety and efficacy prior to clinical use. Stem Cells 2018;36:1311-1328.

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“…Some recent studies have shown that autologous adipose stem cell transplantation can improve osteoarthritis (Bui et al, 2014). Combination of stem cell transplantation and microfracture have also proven to be better than microfracture alone (Nguyen et al, 2016). However, similar to osteochondral allograft transplantation and autologous chondrocyte implantation, stem cell transplantation is expensive but yields promising results in clinical trials.…”

Section: Introductionmentioning

confidence: 99%

Autologous osteochondral transplantation for treatment of cartilage defects in osteoarthritic knee: preliminary results

Bui

Nguyen

et al. 2016

Biomed Res Ther

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Introduction: Osteoarthritis is a contributing factor for pain and loss of function of the knee. Osteoarthritis results in many damages to the knee; one of the most common damages that is difficult to recover is cartilage injury. This study aims to apply autologous osteochondral transplantation (OAT) under knee arthroscopy for the treatment of knee cartilage defects. Methods: This was a prospective, descriptive and non-controlled study. Patients were diagnosed as having osteoarthritis, as confirmed by 1cm 2 -3cm 2 cartilage defects. Arthroscopic OAT was performed on each patient. Treatment efficacy and safety were evaluated based on Lysholm, Oxford Knee Scores (OKS) and pain scales (VAS) after 3, 6, 12 and 18 months. Results: From 3/2014 -8/2016, 61 cases (54 women and 7 men) participated in the study. The average age was 55 ± 8 years old. Most cases had cartilage defects in the medial condyle. Results showed that Lysholm, OKS scores and VAS scales improved after 12 months of treatment. Of the cases, 33 of 61 were followed out to 18 months; these patients showed improvement in knee function and pain scores. There was 1 case with incomplete matching between the plug and receiving site and 1 case with a broken plug. At the final stage of monitoring, there were no patients who experienced complications, such as broken instruments or fracture of condyle, nor who experienced early postoperative complications, such as infection and bleeding. Conclusion: Autologous osteochondral transplantation via arthroscopy is a safe and promising method for the treatment of knee cartilage defects in patients with average osteoarthritis.

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Comparative Clinical Observation of Arthroscopic Microfracture in the Presence and Absence of a Stromal Vascular Fraction Injection for Osteoarthritis

Cited by 99 publications

References 81 publications

Expanded autologous adipose derived stem cell transplantation for type 2 diabetes mellitus

Expanded autologous adipose derived stem cell transplantation for type 2 diabetes mellitus

Concise Review: Using Fat to Fight Disease: A Systematic Review of Nonhomologous Adipose-Derived Stromal/Stem Cell Therapies

Autologous osteochondral transplantation for treatment of cartilage defects in osteoarthritic knee: preliminary results

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