Comparative Cytogenetic Study of Two Sister Species of Iberian Ground Voles, &lt;i&gt;Microtus (Terricola) duodecimcostatus &lt;/i&gt;and &lt;i&gt;M. (T.) lusitanicus&lt;/i&gt; (Rodentia, Cricetidae)

Gornung, Ekaterina; Castiglia, Riccardo; Rovatsos, Michail; Marchal, Juan A.; Guardia-Quiles, R. Díaz de la; Sánchez, Antonio

doi:10.1159/000321572

Cited by 16 publications

(11 citation statements)

References 51 publications

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“…gregalis (six) (Figs 1 and 3E–3G). Previously, it was hypothesized using the ground vole as an example that the “primitive” (ancestral) karyotype is defined by the high number of NORs, whereas species with extensively rearranged karyotypes have a lower number of NORs [51]. Although we have previously shown the hypothesis of multiple NORs as a characteristic of the primitive state this does not hold in other species of voles [25].…”

Section: Discussionmentioning

confidence: 73%

Rapid Karyotype Evolution in Lasiopodomys Involved at Least Two Autosome – Sex Chromosome Translocations

et al. 2016

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The generic status of Lasiopodomys and its division into subgenera Lasiopodomys (L. mandarinus, L. brandtii) and Stenocranius (L. gregalis, L. raddei) are not generally accepted because of contradictions between the morphological and molecular data. To obtain cytogenetic evidence for the Lasiopodomys genus and its subgenera and to test the autosome to sex chromosome translocation hypothesis of sex chromosome complex origin in L. mandarinus proposed previously, we hybridized chromosome painting probes from the field vole (Microtus agrestis, MAG) and the Arctic lemming (Dicrostonyx torquatus, DTO) onto the metaphases of a female Mandarin vole (L. mandarinus, 2n = 47) and a male Brandt's vole (L. brandtii, 2n = 34). In addition, we hybridized Arctic lemming painting probes onto chromosomes of a female narrow-headed vole (L. gregalis, 2n = 36). Cross-species painting revealed three cytogenetic signatures (MAG12/18, 17a/19, and 22/24) that could validate the genus Lasiopodomys and indicate the evolutionary affinity of L. gregalis to the genus. Moreover, all three species retained the associations MAG1bc/17b and 2/8a detected previously in karyotypes of all arvicolins studied. The associations MAG2a/8a/19b, 8b/21, 9b/23, 11/13b, 12b/18, 17a/19a, and 5 fissions of ancestral segments appear to be characteristic for the subgenus Lasiopodomys. We also validated the autosome to sex chromosome translocation hypothesis on the origin of complex sex chromosomes in L. mandarinus. Two translocations of autosomes onto the ancestral X chromosome in L. mandarinus led to a complex of neo-X1, neo-X2, and neo-X3 elements. Our results demonstrate that genus Lasiopodomys represents a striking example of rapid chromosome evolution involving both autosomes and sex chromosomes. Multiple reshuffling events including Robertsonian fusions, chromosomal fissions, inversions and heterochromatin expansion have led to the formation of modern species karyotypes in a very short time, about 2.4 MY.

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Section: Discussionmentioning

confidence: 73%

Rapid Karyotype Evolution in Lasiopodomys Involved at Least Two Autosome – Sex Chromosome Translocations

et al. 2016

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“…The topology of rRNA genes is a popular marker for studying the karyotype evolution (e.g. Gornung et al ., ; Mezzasalma et al ., ; Pucci et al ., ; Rovatsos et al ., ; Sadílek et al ., ; Sember et al ., ). Iguanas possess NORs (or more precisely of 28S rRNA genes) most commonly on a single pair of chromosomes (e.g.…”

Section: Discussionmentioning

confidence: 99%

Minute Y chromosomes and karyotype evolution in Madagascan iguanas (Squamata: Iguania: Opluridae)

Altmanová

Rovatsos

Kratochvíl

et al. 2016

Biol. J. Linn. Soc.

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Iguanas (Pleurodonta) are predominantly distributed in the New World, but one previously cytogenetically understudied family, Opluridae, is endemic to Madagascar and the adjacent Grand Comoro archipelago. The aim of our contribution is to fill a gap in the cytogenetic understanding of this biogeographically puzzling lineage. Based on examination of six species, we found that oplurids are rather conservative in karyotype, which is composed of 36 chromosomes as in most iguanas. However, the species differ in the position of the nucleolar organizer region and heterochromatic blocks and in the accumulation and distribution of interstitial telomeric sequences (ITSs), which suggests cryptic intra-and interchromosomal rearrangements. All tested species share the XY sex-determining system homologous to most other iguana families. The oplurid Y chromosome is degenerated, very small in size but mostly euchromatic. Fluorescence in situ hybridization with probes composed of microsatellite motifs revealed variability among species in the accumulation of particular repeats on the Y chromosome. This variability accounts for the differences in the detection of sex chromosomes across the species of the family using comparative genome hybridization (CGH) technique. Our study demonstrates the limits of the commonly used CGH technique to uncover sex chromosomes even in organisms with heteromorphic and sequentially largely differentiated sex chromosomes.

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“…Other species of rodents which possess particularly large X chromosomes include e.g. Rattus natalensis (Huang and Strong 1962) and several of Microtus species (Marchal et al 2004;Gornung et al 2011). "Quadruplicate-X" (20% of haploid set), the longest X chromosome in Microtus species, was established in M. agrestis (Wolf et al 1965;Nanda et al 1988).…”

Section: Discussionmentioning

confidence: 99%

Karyotyping ofChinchilla lanigeraMol. (Rodentia, Chinchillidae)

et al. 2015

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There is relatively little information about the karyotype structure of the chinchilla (Chinchilla lanigera Mol.), an endemic South American rodent with 2n = 64 chromosomes and "duplicate-type X chromosome". The species, endangered in nature, is a popular domesticated animal providing one of the most valuable furs in the world. In the present study, detailed karyotype analysis of the domestic chinchilla was performed using selected methods of differential chromosome staining (G-banding, C-banding, C-banding/DAPI, CMA 3 /DA/DAPI, Ag-NOR staining) as well as fluorescence in situ hybridization (FISH) with rDNA and telomeric (TTAGGG) n repetitive probes. The analysed specimens showed 59 metacentric and five submetacentric chromosomes. C-banding revealed mainly centromeric distribution of heterochromatin on the autosomes, interstitial and centromeric C-positive bands on the X chromosome and almost entirely heterochromatic nature of the Y chromosome. The average amount of heterochromatin in the haploid autosome set (31A) was 11.44%, whereas in the X chromosome 39.84%. Two active nucleolar organizer regions (NORs) and 45S rDNA repeats were located within one pair of big autosomes. In respect of morphology and G-banding patterns, the chinchilla chromosomes were arranged in homologous pairs and a G-banded karyotype was proposed. The obtained results could be the first step towards determination of the standard karyotype for the breeding chinchilla.

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Comparative Cytogenetic Study of Two Sister Species of Iberian Ground Voles, <i>Microtus (Terricola) duodecimcostatus </i>and <i>M. (T.) lusitanicus</i> (Rodentia, Cricetidae)

Cited by 16 publications

References 51 publications

Rapid Karyotype Evolution in Lasiopodomys Involved at Least Two Autosome – Sex Chromosome Translocations

Rapid Karyotype Evolution in Lasiopodomys Involved at Least Two Autosome – Sex Chromosome Translocations

Minute Y chromosomes and karyotype evolution in Madagascan iguanas (Squamata: Iguania: Opluridae)

Karyotyping ofChinchilla lanigeraMol. (Rodentia, Chinchillidae)

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