Comparative cytogenetics between two species of the family Pseudopimelodidae (Siluriformes): occurrence of natural triploidy and supernumerary chromosomes

Gouveia, Juceli Gonzalez; Moraes, Vivian Patrícia Oliveira de; Pires, Larissa Bettin; Rosa, Renata da; Dias, Ana Lúcia

doi:10.1007/s10616-013-9676-x

“…Recently, robust molecular studies resolved their phylogenetic uncertainties by placing this and other Percomorpha representatives into 11 orders within a new supraordinal group called Percomorpharia, even though Perciformes remained as the most species-rich order ( Betancur et al 2013 ). Nonetheless, in spite of their ecological and evolutionary relevance, the marine representatives from this large fish group remain poorly studied from a cytogenetic viewpoint ( Galetti et al 2006 ) when compared to typical freshwater families ( Balen et al 2013 , Cardoso et al 2013 , Gouveia et al 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Chromosomal stasis in distinct families of marine Percomorpharia from South Atlantic

Paim¹,

Almeida²,

Affonso³

et al. 2017

CCG

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The weakness of physical barriers in the marine environment and the dispersal potential of fish populations have been invoked as explanations of the apparent karyotype stasis of marine Percomorpha, but several taxa remain poorly studied cytogenetically. To increase the chromosomal data in this fish group, we analyzed cytogenetically three widespread Atlantic species from distinct families: Chaetodipterus faber Broussonet, 1782 (Ephippidae), Lutjanus synagris Linnaeus, 1758 (Lutjanidae) and Rypticus randalli Courtenay, 1967 (Serranidae). The three species shared a karyotype composed of 2n=48 acrocentric chromosomes, single nucleolus organizer regions (NORs) and reduced amounts of centromeric heterochromatin. A single NOR-bearing pair was identified in all species by physical mapping of 18S rDNA while non-syntenic 5S rRNA genes were located at centromeric region of a single pair. The similar karyotypic macrostructure observed in unrelated groups of Percomorpharia reinforces the conservative karyoevolution of marine teleosteans. Nonetheless, the species could be differentiated based on the pair bearing ribosomal cistrons, revealing the importance of microstructural analyses in species with symmetric and stable karyotypes.

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“…Therefore, despite few species analyzed in Pseudopimelodidae family, 5S rDNA sites do not seem to follow a pattern. This can be attributed to the action of other genomic elements, since the variation of rDNA genes has also been described in karyotypes of different species within this family, including species of the same genus (Gouveia et al 2015).…”

Section: Discussionmentioning

confidence: 90%

“…The chromosome structure of Imparfinis schubarti and Microglanis cottoides were analyzed by Gouveia et al (2013Gouveia et al ( , 2015, respectively. Imparfinis schubarti has 58 chromosomes distributed in 30 m ?…”

Section: T Pcr and Fish With Rdna 5s Probementioning

confidence: 99%

“…Imparfinis mirini Haseman 1911 and Imparfinis minutus (Lutken 1874) analyzed by Ferreira et al (2014) also showed the same synteny, besides presenting another small metacentric pair, bearer of a single 5S rDNA site in the region near the centromere. In the Pseudopimelodidae family, which has a close phylogenetic relationship with Heptapteridae, hybridization data are even scarcer (Gouveia et al 2015). The location of 5S rDNA sites are known only in Lophiosilurus alexandri Steindachner, 1877, evidenced in the terminal region of a pair of subtelocentric chromosome (Marques et al 2008).…”

Section: Introductionmentioning

confidence: 99%

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Isolation and characterization of 5S rDNA sequences in catfishes genome (Heptapteridae and Pseudopimelodidae): perspectives for rDNA studies in fish by C0t method

Gouveia

¹

,

Wolf

²

,

Moraes-Manécolo

³

et al. 2016

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Sequences of 5S ribosomal RNA (rRNA) are extensively used in fish cytogenomic studies, once they have a flexible organization at the chromosomal level, showing inter-and intra-specific variation in number and position in karyotypes. Sequences from the genome of Imparfinis schubarti (Heptapteridae) were isolated, aiming to understand the organization of 5S rDNA families in the fish genome. The isolation of 5S rDNA from the genome of I. schubarti was carried out by reassociation kinetics (C 0 t) and PCR amplification. The obtained sequences were cloned for the construction of a micro-library. The obtained clones were sequenced and hybridized in I. schubarti and Microglanis cottoides (Pseudopimelodidae) for chromosome mapping. An analysis of the sequence alignments with other fish groups was accomplished.Both methods were effective when using 5S rDNA for hybridization in I. schubarti genome. However, the C 0 t method enabled the use of a complete 5S rRNA gene, which was also successful in the hybridization of M. cottoides. Nevertheless, this gene was obtained only partially by PCR. The hybridization results and sequence analyses showed that intact 5S regions are more appropriate for the probe operation, due to conserved structure and motifs. This study contributes to a better understanding of the organization of multigene families in catfish's genomes.

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“…Recently, robust molecular studies resolved their phylogenetic uncertainties by placing this and other Percomorpha representatives into 11 orders within a new supraordinal group called Percomorpharia, even though Perciformes remained as the most species-rich order (Betancur et al 2013). Nonetheless, in spite of their ecological and evolutionary relevance, the marine representatives from this large fish group remain poorly studied from a cytogenetic viewpoint (Galetti et al 2006) when compared to typical freshwater families (Balen et al 2013, Cardoso et al 2013, Gouveia et al 2013.…”

Section: Introductionmentioning

confidence: 99%

Chromosomal stasis in distinct families of marine Percomorpharia from South Atlantic

Paim

¹

,

Almeida

²

,

Affonso

³

et al. 2017

CCG

3

0

View full text Add to dashboard Cite

The weakness of physical barriers in the marine environment and the dispersal potential of fish populations have been invoked as explanations of the apparent karyotype stasis of marine Percomorpha, but several taxa remain poorly studied cytogenetically. To increase the chromosomal data in this fish group, we analyzed cytogenetically three widespread Atlantic species from distinct families: Chaetodipterus faber Broussonet, 1782 (Ephippidae), Lutjanus synagris Linnaeus, 1758 (Lutjanidae) and Rypticus randalli Courtenay, 1967 (Serranidae). The three species shared a karyotype composed of 2n=48 acrocentric chromosomes, single nucleolus organizer regions (NORs) and reduced amounts of centromeric heterochromatin. A single NOR-bearing pair was identified in all species by physical mapping of 18S rDNA while non-syntenic 5S rRNA genes were located at centromeric region of a single pair. The similar karyotypic macrostructure observed in unrelated groups of Percomorpharia reinforces the conservative karyoevolution of marine teleosteans. Nonetheless, the species could be differentiated based on the pair bearing ribosomal cistrons, revealing the importance of microstructural analyses in species with symmetric and stable karyotypes.

show abstract

Comparative cytogenetics between two species of the family Pseudopimelodidae (Siluriformes): occurrence of natural triploidy and supernumerary chromosomes

Cited by 4 publications

References 25 publications

Chromosomal stasis in distinct families of marine Percomorpharia from South Atlantic

Chromosomal stasis in distinct families of marine Percomorpharia from South Atlantic

Isolation and characterization of 5S rDNA sequences in catfishes genome (Heptapteridae and Pseudopimelodidae): perspectives for rDNA studies in fish by C0t method

Chromosomal stasis in distinct families of marine Percomorpharia from South Atlantic

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