Comparative cytology combined with transcriptomic and metabolomic analyses of Solanum nigrum L. in response to Cd toxicity

Wang, Juncai; Chen, Xunfeng; Chu, Shaoli; You, Yimin; Chi, Yaowei; Wang, Renyuan; Yang, Xijia; Hayat, Kashif; Zhang, Dan; Zhou, Pei

doi:10.1016/j.jhazmat.2021.127168

Cited by 106 publications

(71 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, upregulated phenylpropanoid biosynthesis could facilitate the fixation of Cd on the lignin of the cell wall [ 28 ]. Consistent with the results of Wang et al [ 29 ], many DEGs were associated with the phenylpropanoid biosynthesis pathway in the current study. Therefore, the DEGs of these hub modules were further analyzed, and most of these DEGs were related to lignin biosynthesis.…”

Section: Discussionsupporting

confidence: 93%

See 1 more Smart Citation

Dynamic and Comparative Transcriptome Analyses Reveal Key Factors Contributing to Cadmium Tolerance in Broomcorn Millet

Li¹,

Zhang²,

Zhang³

et al. 2022

IJMS

View full text Add to dashboard Cite

Broomcorn millet (Panicum miliaceum L.) has great potential in Cd phytoextraction, but its mechanisms are largely unknown. Two contrasting broomcorn millet varieties, ‘Ningmi6’ (Cd-sensitive variety) and ‘4452’ (Cd-tolerant variety), were investigated through morphological, physiological, and transcriptomic analyses to determine the factors responsible for their differential Cd tolerance and translocation. The Cd-tolerant variety can accumulate more Cd, and its cell wall and vacuole component Cd proportions were higher compared with the Cd-sensitive variety. Under Cd stress, the glutathione content and peroxidase activity of the Cd-tolerant variety were significantly higher than those of the Cd-sensitive variety. Additionally, weighted gene co-expression network analysis (WGCNA) revealed hub modules that were associated with Cd stress and/or variety. Notably, genes involved in these hub modules were significantly enriched for roles in glutathione metabolism, phenylpropanoid biosynthesis, ABC transport, and metal ion transport process. These results suggested that regulation of genes associated with cell wall precipitation and vacuole compartmentalization may increase Cd tolerance and reduce Cd translocation in the Cd-tolerant variety, although it can absorb more Cd. This study provides a foundation for exploring molecular mechanisms of Cd tolerance and transport in broomcorn millet and new insights into improving Cd phytoremediation with this crop through genetic engineering.

show abstract

Section: Discussionsupporting

confidence: 93%

“…Glutathione (GSH) metabolism was one of the key pathways involved in the response of broomcorn millet to Cd stress, which was consistent with previous studies on Solanum nigrum [ 29 ]. GSH, a thiol-containing compound, is a bioactive tripeptide involved in many aspects of plant metabolism.…”

Section: Discussionsupporting

confidence: 89%

Dynamic and Comparative Transcriptome Analyses Reveal Key Factors Contributing to Cadmium Tolerance in Broomcorn Millet

Li¹,

Zhang²,

Zhang³

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…2 ). Furthermore, Cd toxicity is often accompanied by severe oxidative damage, which seriously represses plant growth [ 49 ]. In this study, Cd stress markedly reduced Fe, Mn and Zn in the roots and Mn and Cu in the leaves (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Plants adopt efficient strategies, including molecular regulation, signal transduction, and alteration of metabolic pathways, to resist Cd toxicity [ 42 , 49 ]. Cd stress induced higher metabolites in the roots than in the leaves (Fig.…”

Section: Discussionmentioning

confidence: 99%

Comparative physiological and metabolomic analyses reveal that Fe3O4 and ZnO nanoparticles alleviate Cd toxicity in tobacco

Zou

Wang

et al. 2022

J Nanobiotechnol

View full text Add to dashboard Cite

Background Heavy metals repress tobacco growth and quality, and engineered nanomaterials have been used for sustainable agriculture. However, the underlying mechanism of nanoparticle-mediated cadmium (Cd) toxicity in tobacco remains elusive. Results Herein, we investigated the effects of Fe3O4 and ZnO nanoparticles (NPs) on Cd stress in tobacco cultivar ‘Yunyan 87’ (Nicotianatabacum). Cd severely repressed tobacco growth, whereas foliar spraying with Fe3O4 and ZnO NPs promoted plant growth, as indicated by enhancing plant height, root length, shoot and root fresh weight under Cd toxicity. Moreover, Fe3O4 and ZnO NPs increased, including Zn, K and Mn contents, in the roots and/or leaves and facilitated seedling growth under Cd stress. Metabolomics analysis showed that 150 and 76 metabolites were differentially accumulated in roots and leaves under Cd stress, respectively. These metabolites were significantly enriched in the biosynthesis of amino acids, nicotinate and nicotinamide metabolism, arginine and proline metabolism, and flavone and flavonol biosynthesis. Interestingly, Fe3O4 and ZnO NPs restored 50% and 47% in the roots, while they restored 70% and 63% in the leaves to normal levels, thereby facilitating plant growth. Correlation analysis further indicated that these metabolites, including proline, 6-hydroxynicotinic acid, farrerol and quercetin-3-O-sophoroside, were significantly correlated with plant growth. Conclusions These results collectively indicate that metal nanoparticles can serve as plant growth regulators and provide insights into using them for improving crops in heavy metal-contaminated areas. Graphical Abstract

show abstract

“…Comprehensive metabolic pathway enrichment analyses of these genes, proteins and metabolites pinpointed to three TF families ( GRAS , GRF , and REV ) and lignin biosynthesis pathway as responsible for the inhibition of tiller development in low-tillering wheat cultivars ( Wang Z. et al, 2019 ). Besides, conjoint analysis (coupling comparative cytology with transcriptomic and metabolomic approaches) to understand the mechanisms underlying Solanum nigrum L. response to cadmium toxicity revealed key differentially expressed genes and metabolites, including laccase, peroxidase, D-fructose, and cellobiose, that were associated with cell wall biosynthesis, implying that the cell wall biosynthesis pathway plays a central role in Cd detoxification in Solanum nigrum ( Wang et al, 2022 ). Combined transcriptomic and metabolomic approaches applied in maize to analyze gene regulatory networks modulating Rp1-D21 mutant-mediated hypersensitive pathogen defense response revealed that four uridinediphosphate-dependent glycosyltransferase (UGT) ( ZmUGTs) genes were highly expressed, whilst the SA biosynthesis and phenylpropanoid biosynthesis pathways were induced at both the transcriptional and metabolic levels, suggesting that ZmUGT genes may be involved in maize defense response by regulating SA homeostasis ( Ge et al, 2021 ).…”

Section: Omics Technologies Integrated With Modern Plant Breeding Methods In a Systems Biology Approach For Crop Improvementmentioning

confidence: 99%

Omics-Facilitated Crop Improvement for Climate Resilience and Superior Nutritive Value

et al. 2021

View full text Add to dashboard Cite

Novel crop improvement approaches, including those that facilitate for the exploitation of crop wild relatives and underutilized species harboring the much-needed natural allelic variation are indispensable if we are to develop climate-smart crops with enhanced abiotic and biotic stress tolerance, higher nutritive value, and superior traits of agronomic importance. Top among these approaches are the “omics” technologies, including genomics, transcriptomics, proteomics, metabolomics, phenomics, and their integration, whose deployment has been vital in revealing several key genes, proteins and metabolic pathways underlying numerous traits of agronomic importance, and aiding marker-assisted breeding in major crop species. Here, citing several relevant examples, we appraise our understanding on the recent developments in omics technologies and how they are driving our quest to breed climate resilient crops. Large-scale genome resequencing, pan-genomes and genome-wide association studies are aiding the identification and analysis of species-level genome variations, whilst RNA-sequencing driven transcriptomics has provided unprecedented opportunities for conducting crop abiotic and biotic stress response studies. Meanwhile, single cell transcriptomics is slowly becoming an indispensable tool for decoding cell-specific stress responses, although several technical and experimental design challenges still need to be resolved. Additionally, the refinement of the conventional techniques and advent of modern, high-resolution proteomics technologies necessitated a gradual shift from the general descriptive studies of plant protein abundances to large scale analysis of protein-metabolite interactions. Especially, metabolomics is currently receiving special attention, owing to the role metabolites play as metabolic intermediates and close links to the phenotypic expression. Further, high throughput phenomics applications are driving the targeting of new research domains such as root system architecture analysis, and exploration of plant root-associated microbes for improved crop health and climate resilience. Overall, coupling these multi-omics technologies to modern plant breeding and genetic engineering methods ensures an all-encompassing approach to developing nutritionally-rich and climate-smart crops whose productivity can sustainably and sufficiently meet the current and future food, nutrition and energy demands.

show abstract

Comparative cytology combined with transcriptomic and metabolomic analyses of Solanum nigrum L. in response to Cd toxicity

Cited by 106 publications

References 71 publications

Dynamic and Comparative Transcriptome Analyses Reveal Key Factors Contributing to Cadmium Tolerance in Broomcorn Millet

Dynamic and Comparative Transcriptome Analyses Reveal Key Factors Contributing to Cadmium Tolerance in Broomcorn Millet

Comparative physiological and metabolomic analyses reveal that Fe3O4 and ZnO nanoparticles alleviate Cd toxicity in tobacco

Omics-Facilitated Crop Improvement for Climate Resilience and Superior Nutritive Value

Contact Info

Product

Resources

About