Comparative decay of culturable faecal indicator bacteria, microbial source tracking marker genes, and enteric pathogens in laboratory microcosms that mimic a sub-tropical environment

Ahmed, Warish; Toze, Simon; Veal, Cameron; Fisher, Paul; Zhang, Qian; Zhu, Zhigang; Staley, Christopher; Sadowsky, Michael J.

doi:10.1016/j.scitotenv.2020.141475

Cited by 33 publications

(12 citation statements)

References 53 publications

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“…4,29−31 Previous work has found that temperature modifies the decay of crAssphage based on sewage-spiked mesocosms sampled during average winter (14.5 °C) and summer temperatures (25 °C) in Spain, 35,51 and from sewage in freshwater microcosms representing winter and summer kept at 15 and 25 °C. 31 Our work extends the knowledge of this relationship to real-world wastewater matrices. The decay modification by wastewater temperature has also been observed for SARS-CoV-2 4,27 and murine hepatitis virus RNA in untreated wastewater.…”

Section: The Ability To Capture Signals From Distributed Locations Is...mentioning

confidence: 66%

“…Due to the evidence of temperature-modified decay of microbial markers, ,,, and for crAssphage in particular, , we expand the second SPM approach (SEDC-T). We let α 90 exponentially decrease with wastewater temperature by setting α 90 = α 90,T exp(γ 1 T i ) where T i is the z-scored wastewater temperature (with a mean of 0 and standard deviation of 1), γ 1 < 0 is a fitted hyperparameter that captures the exponential decrease of α 90 with z-scored temperature, and α 90,T is the value of α 90 at the average wastewater temperature (i.e., when Ti = 0) of 18.3 °C for this study (see Supporting Information 3 for more descriptive statistics).…”

Section: Materials and Methodsmentioning

confidence: 99%

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Characterizing Spatial Information Loss for Wastewater Surveillance Using crAssphage: Effect of Decay, Temperature, and Population Mobility

Wiesner-Friedman,

Brinkman,

Wheaton

et al. 2023

Environ. Sci. Technol.

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Section: The Ability To Capture Signals From Distributed Locations Is...mentioning

confidence: 66%

Section: Materials and Methodsmentioning

confidence: 99%

Characterizing Spatial Information Loss for Wastewater Surveillance Using crAssphage: Effect of Decay, Temperature, and Population Mobility

Wiesner-Friedman,

Brinkman,

Wheaton

et al. 2023

Environ. Sci. Technol.

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“…Previous reports also noted the extended persistence of coliphages compared to FIB in freshwaters [ 86 , 87 , 88 ] and marine environments [ 87 , 88 , 89 ]. A trend of faster decay of culturable FIB compared to viral pathogens (i.e., adenovirus 40/41 and coxsackie A9) [ 90 , 91 , 92 , 93 ] further supports the need for viral indicator(s).…”

Section: Discussionmentioning

confidence: 91%

The Effect of Protozoa Indigenous to Lakewater and Wastewater on Decay of Fecal Indicator Bacteria and Coliphage

2023

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Fecal indicator bacteria (FIB: Escherichia coli and enterococci) are used to assess recreational water quality. Viral indicators (i.e., somatic and F+ coliphage), could improve the prediction of viral pathogens in recreational waters, however, the impact of environmental factors, including the effect of predatory protozoa source, on their survival in water is poorly understood. We investigated the effect of lakewater or wastewater protozoa, on the decay (decreasing concentrations over time) of culturable FIB and coliphages under sunlight and shaded conditions. FIB decay was generally greater than the coliphages and was more rapid when indicators were exposed to lake vs. wastewater protozoa. F+ coliphage decay was the least affected by experimental variables. Somatic coliphage decayed fastest in the presence of wastewater protozoa and sunlight, though their decay under shaded conditions was-10-fold less than F+ after 14 days. The protozoa source consistently contributed significantly to the decay of FIB, and somatic, though not the F+ coliphage. Sunlight generally accelerated decay, and shade reduced somatic coliphage decay to the lowest level among all the indicators. Differential responses of FIB, somatic, and F+ coliphages to environmental factors support the need for studies that address the relationship between the decay of coliphages and viral pathogens under environmentally relevant conditions.

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“…Furthermore, feces found and sampled in Yarawalia appeared fresh and moist, whereas droppings in the other two villages appeared old and dry. This difference could have affected the detection of LASV as older feces likely had longer exposure to high temperatures inside houses leading to viral decay [ 18 , 19 ].…”

Section: Resultsmentioning

confidence: 99%

Detection of Lassa virus in wild rodent feces: Implications for Lassa fever burden within households in the endemic region of Faranah, Guinea

et al. 2021

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Lassa arenavirus (LASV) is the cause of Lassa Fever in humans in West Africa. The multimammate mouse ( Mastomys natalensis ) is a reservoir host of LASV and the primary source of human infections. Humans are assumed to become infected due to contact with this animal or its excretions. Thus far, the available literature does not describe the sampling of feces as a means to detect LASV in M. natalensis populations. More evidence is needed to know if feces of naturally infected M. natalensis can be LASV-positive and an exposure risk to humans. This study sampled feces deposits in households from three villages in the LASV-endemic region of Faranah, Guinea. PCR analysis found 10 out of 88 samples to be positive for LASV, and sequencing showed clustering to previously identified Yarawelia and Dalafilani strains. We conclude that feces sampling is a viable, non-invasive method for the determination and sequencing of LASV strains.

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Comparative decay of culturable faecal indicator bacteria, microbial source tracking marker genes, and enteric pathogens in laboratory microcosms that mimic a sub-tropical environment

Cited by 33 publications

References 53 publications

Characterizing Spatial Information Loss for Wastewater Surveillance Using crAssphage: Effect of Decay, Temperature, and Population Mobility

Characterizing Spatial Information Loss for Wastewater Surveillance Using crAssphage: Effect of Decay, Temperature, and Population Mobility

The Effect of Protozoa Indigenous to Lakewater and Wastewater on Decay of Fecal Indicator Bacteria and Coliphage

Detection of Lassa virus in wild rodent feces: Implications for Lassa fever burden within households in the endemic region of Faranah, Guinea

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