Comparative diagnostic efficacy of recombinant LLO and PI-PLC-based ELISAs for detection of listeriosis in animals

Suryawanshi, Rahul D.; Malik, S.V.S.; Jayarao, Bhushan M.; Chaudhari, S. P.; Savage, E. A.; Vergis, Jess; Kurkure, N. V.; Barbuddhe, Sukhadeo B.; Rawool, Deepak B.

doi:10.1016/j.mimet.2017.04.005

Cited by 6 publications

(4 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…with a random effects proportion of 0. [64,65,67]. In the present study, it was also observed that the prevalence of Streptococcus suis was on par with the findings of several researchers [90,94,96].…”

Section: Discussionsupporting

confidence: 89%

“…Serological and molecular epidemiological studies can help in elucidating the genetic diversity and evolution of these pathogens [99]. It has also been observed that the studies included in the present meta-analysis commonly used techniques like biochemical tests and PCR [44,56,97] followed by ELISA [34,37,64] and lateral flow assays [33] for the detection of bacterial pathogens. It is very much desired that longitudinal studies are needed to monitor the changes in prevalence over time and to assess the effectiveness of control measures.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Meta-Analysis of the Prevalence of Porcine Zoonotic Bacterial Pathogens in India: A 13-Year (2010–2023) Study

Rajkhowa,

Sonowal,

Borthakur

et al. 2023

Pathogens

View full text Add to dashboard Cite

The presence of bacterial pathogens such as Brucella spp., Clostridium spp., E. coli, Listeria monocytogenes, Salmonella spp., Staphylococcus spp., and Streptococcus suis not only hampers pig production but also carries significant zoonotic implications. The present study aims to conduct a comprehensive meta-analysis spanning over 13 years (2010–2023) to ascertain the prevalence of these zoonotic bacterial pathogens in Indian pig populations. The study seeks to synthesize data from diverse geographic regions within India and underscores the relevance of the One Health framework. A systematic search of electronic databases was meticulously performed. Inclusion criteria encompassed studies detailing zoonotic bacterial pathogen prevalence in pigs within India during the specified timeframe. Pertinent information including authors, publication year, geographical location, sampling techniques, sample sizes, and pathogen-positive case counts were meticulously extracted. The meta-analysis of zoonotic bacterial pathogens in Indian pig populations (2010–2023) unveiled varying prevalence rates: 9% Brucella spp., 22% Clostridium spp., 19% E. coli, 12% Listeria monocytogenes, 10% Salmonella spp. and Streptococcus suis, and 24% Staphylococcus spp. The application of random effects further revealed additional variability: 6% Brucella spp., 23% Clostridium spp., 24% E. coli, 14% Listeria monocytogenes, 10% Salmonella spp. and Streptococcus suis, and 35% Staphylococcus spp. Notably, the observed heterogeneity (I2) varied significantly from 87% to 99%. The meta-analysis findings underscore the pervasive nature of these diseases throughout India’s pig populations, accentuating the substantial impact of these pathogens on pig health and the potential for zoonotic transmission. The present study reinforces the importance of the adoption of a comprehensive One Health approach that acknowledges the intricate interplay between animal, human and environmental health.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Meta-Analysis of the Prevalence of Porcine Zoonotic Bacterial Pathogens in India: A 13-Year (2010–2023) Study

Rajkhowa,

Sonowal,

Borthakur

et al. 2023

Pathogens

View full text Add to dashboard Cite

show abstract

“…The antigenic potential of secreted proteins, such as listeriolysin and phosphatidylinositol-specific phospholipase C (PI-PLC), both of which are necessary for the escape of the pathogen from the phagocytic vacuoles of the host, has also been employed for antibody development. In the case of listeriolysin, quite promising immunological approaches for listeriosis diagnosis have been developed [ 13 , 14 ]. In contrast, the use of PI-PLC resulted in extended cross-reactivity due to its conserved nature among listeriae and non- Listeria genera, such as Bacillus spp.…”

Section: Surface-residing Molecular Targetsmentioning

confidence: 99%

Molecular Targets for Foodborne Pathogenic Bacteria Detection

Paramithiotis

2023

Pathogens

View full text Add to dashboard Cite

The detection of foodborne pathogenic bacteria currently relies on their ability to grow on chemically defined liquid and solid media, which is the essence of the classical microbiological approach. Such procedures are time-consuming and the quality of the result is affected by the selectivity of the media employed. Several alternative strategies based on the detection of molecular markers have been proposed. These markers may be cell constituents, may reside on the cell envelope or may be specific metabolites. Each marker provides specific advantages and, at the same time, suffers from specific limitations. The food matrix and chemical composition, as well as the accompanying microbiota, may also severely compromise detection. The aim of the present review article is to present and critically discuss all available information regarding the molecular targets that have been employed as markers for the detection of foodborne pathogens. Their strengths and limitations, as well as the proposed alleviation strategies, are presented, with particular emphasis on their applicability in real food systems and the challenges that are yet to be effectively addressed.

show abstract

“…Over two decades ago, even its existence in India in environmental and clinical sources was in doubt, with very few studies documenting unambiguous detection of this bacterium (Barbuddhe et al ., 2012). We have been actively engaged for more than a decade in the standardization, development, and evaluation of the methods based on the virulence markers of L. monocytogenes and L. ivanovii , for rapid diagnosis of listeriosis primarily in animals and human beings (Rawool et al ., 2007; Barbuddhe et al ., 2012; Suryawanshi et al ., 2017). The presence of Listeria in different ecological niches have been recorded from India, including foods, soil, vegetables, mangrove swamps, seafood, freshwater fishes, clinical cases, and also insects (Parihar et al ., 2008; Barbuddhe et al ., 2012; Soni et al ., 2014; Doijad et al ., 2018; Kulesh et al ., 2019).…”

Section: Introductionmentioning

confidence: 99%

Ecology of Listeria monocytogenes and Listeria species in India: the occurrence, resistance to biocides, genomic landscape and biocontrol

Barbuddhe

Rawool

Doijad

et al. 2021

Environmental Microbiology

Self Cite

View full text Add to dashboard Cite

Summary Listeria monocytogenes, the causative agent of listeriosis, has been implicated in increasing foodborne outbreaks worldwide. The disease is manifested in various forms ranging from severe sepsis in immune‐compromised individuals, febrile gastroenteritis, still birth, abortions and meningoencephalitis. In India, data from studies on the detection and molecular epidemiological analysis of L. monocytogenes are only recently emerging. The presence of Listeria in different ecological niches has been recorded from India, including foods, soil, vegetables, mangrove swamps, seafood, freshwater fishes, clinical cases, and also insects. The organism has also been isolated from women with spontaneous abortions, miscarriage or recurrent obstetric history, aborted foetuses, animal clinical cases and wildlife samples. A novel species of Listeria has also been characterized. Listeria monocytogenes strains isolated from clinical, environmental, and foods showed biofilm‐forming abilities. Listeria monocytogenes serotype 4b isolates of ST328, a predominant and unique ST observed in India, was repeatedly isolated from different sources, times, and geographical locations. Here, we reviewed the occurrence of Listeria in different sources in India, its resistance to biocides, and provide epidemiological analysis on its genomic landscape.

show abstract

Comparative diagnostic efficacy of recombinant LLO and PI-PLC-based ELISAs for detection of listeriosis in animals

Cited by 6 publications

References 35 publications

Meta-Analysis of the Prevalence of Porcine Zoonotic Bacterial Pathogens in India: A 13-Year (2010–2023) Study

Meta-Analysis of the Prevalence of Porcine Zoonotic Bacterial Pathogens in India: A 13-Year (2010–2023) Study

Molecular Targets for Foodborne Pathogenic Bacteria Detection

Ecology of Listeria monocytogenes and Listeria species in India: the occurrence, resistance to biocides, genomic landscape and biocontrol

Contact Info

Product

Resources

About