Comparative Ecotoxicological Analyses of Spent Phone Batteries on Pseudomonas sp. in Tri Aquatic Environment

Nrior, Renner Renner; Kpormon, Lucky B.

doi:10.9734/cjast/2018/42169

Cited by 4 publications

(14 citation statements)

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“…After incubation, bluish green, circular, convex colonies appeared and Gram staining of the colonies revealed Gram negative rods indicative of Pseudomonas sp. After incubation, the bacterial colonies that grew on the plates were sub-cultured unto fresh nutrient agar plates using the streak plate technique to obtain pure culture of the bacterial isolates as adopted by Nrior and Kpormon [13].…”

Section: Media Used -Pseudomonas Agar Basementioning

confidence: 99%

“…After incubation, the bacterial colonies that grew on the plates were sub-cultured unto fresh nutrient agar plates using the streak plate technique to obtain pure culture of the bacterial isolates as adopted by Nrior and Kpormon [13].…”

Section: Media Used -Bacillus Selective Agar Basementioning

confidence: 99%

“…When the toxic components are found in the soil, they affect the presence of the soil microbial enzymes which are required for the above processes and for organic matter turnover. Key enzymes affected in the soil are dehydrogenase (play a very essential role in the process of organic matter oxidation [13].…”

Section: Introductionmentioning

confidence: 99%

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Enhanced Biodegradation and Release Pattern of Heavy Metal from Spent Laptop Batteries using Pseudomonas and Bacillus Species in Freshwater

Nrior

Nlem

2021

SAJRM

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Aim: To evaluate Enhanced-Biodegradation and Release Pattern of Heavy Metal from Spent Laptop Batteries using Pseudomonas and Bacillus species in Freshwater Study Design: The study employs experimental design, statistical analysis of the data and interpretation. Place and Duration of Study: Freshwater was collected from Isiokpo River, Isiokpo town in Ikwerre L.G.A, Rivers state, Nigeria within co-ordinates 50 02’14’’N and 60 54’50’’E, These samples were transported with ice pack to the Microbiology Laboratory of the Rivers State University, for analyses within 6 hours. Spent laptop batteries were obtained from Ogbunabali Laptops Shoping Complex Garrison area of Port Harcourt, Nigeria. Three sets of the brand of battery (HP, Dell and Acer). Methodology: A total of twelve (12) experimental set-up with three controls (each of the three laptop batteries in freshwater without augmenting organisms) while the other nine were enhanced with augmenting microbes. In step 1, Stock toxicant solution was prepared by soaking the spent Laptop batteries ( of average weight of 300-400g, in two (2) liters of sterilized freshwater in a sterile trough with vented top for aeration, of dimension 12 x 7.5 x 6cm separately for each set-up, The soaking (Toxicant preparation) lasted for 3 months (about 92 days). In step 2, Five hundred milliliters (500ml) of each set-up was transferred into sterile Conical flask plugged with cotton wool perforated for aeration; each was inoculated with five milliliter (5 ml) of the test organisms (Pseudomonas and Bacillus species broth, singly and consortium) separately and monitored for duration 0, 30, 60 and 90 days respectively using the spread plate techniques. The bacterial cultures were incubated at 37°C for 24 hours while fungal cultures were incubated for three (3) days at 35°C. Parameters monitored were Heavy metal (Lithium, Cadmium, Chromium, iron and Lead) concentration, Total Heterotrophic Bacteria, Total Heterotrophic Fungi, Lithium Utilizing Bacteria, Cadmium Utilizing Bacteria, Chromium Utilizing Bacteria, Iron Utilizing Bacteria, Lead Utilizing Bacteria, Lithium Utilizing Fungi, Cadmium Utilizing Fungi, Chromium Utilizing Fungi, Iron Utilizing Fungi, Lead Utilizing Fungi. Results: Enhanced biodegradation and release pattern of heavy metal from spent laptop batteries using Pseudomonas and Bacillus species in freshwater was evaluated and the concentration of heavy metals (Lithium, Cadmium, Chromium, iron and Lead) found associated with the spent laptop batteries (HP, Dell and Acer) were increasing simultaneously with time in all set-ups from day 1 to day 90. The control (without augmenting microbes) has the highest concentration of heavy metals from day 1 to 90 followed by set-ups augmented with Bacillus specie followed by set-ups augmented with Pseudomonas specie, least in set-ups augmented with the consortium of the 2 isolates. The changes in concentration of heavy metals during biodegradation of which Cd < Cr < Li < Fe < Pb respectively, for all spent laptop batteries used in this study. Percentage (%) degradation potential of the consortium of Bacillus species and Pseudomonas species for Li-HP, Li-Dell, and Li-Acer shows a higher percentage (%) release of 22.68%, 37.63% and 24.22% respectively as compared to the individual strains of for Bacillus species and Pseudomonas species. With Pseudomonas species having 10.03%, 18.65%, and 11.24%, followed by Bacillus species having 8.46%, 12.49%, and 7.20% for Li-HP, Li-Dell, and Li-Acer respectively. Conclusion: The study identifies the degradability potential of Bacillus and Pseudomonas species to degrade spent laptop battery in freshwater. it shows that the consortium was able to degrade the batteries better than the individual strains. It is recommended that spent laptop batteries discharged into aquatic environment should be enhanced with broth culture of eco-friendly species of Pseudomonas and Bacillus for quick degradation.

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Section: Media Used -Pseudomonas Agar Basementioning

confidence: 99%

Section: Media Used -Bacillus Selective Agar Basementioning

confidence: 99%

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Enhanced Biodegradation and Release Pattern of Heavy Metal from Spent Laptop Batteries using Pseudomonas and Bacillus Species in Freshwater

Nrior

Nlem

2021

SAJRM

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“…The bio-augmenting organisms (Bacillus and Pseudomonas species) were isolated from the fresh water using standard microbiological methods (spread plate method) as described by Prescott, et al [13]. An aliquot (0.1 ml) of the fresh water sample was aseptically inoculated into properly dried nutrient agar plates (15 ml in each plate) in duplicate, spread evenly using flamed bent rod and incubated at 37°C for 24 hours, after incubation, the bacterial colonies that grew on the plates were sub-cultured unto fresh nutrient agar plates using the streak plate technique to obtain pure culture of the bacterial isolates as adopted by [14].…”

Section: Isolation Of the Test Organismsmentioning

confidence: 99%

“…After incubation, the bacterial colonies that grew on the plates were counted and average taken. Total Heterotrophic Bacteria (THB) Counts was then taken and expressed as colony forming unit per millilitter using the equation below as adopted by Nrior and Kpormon [14]. Dilution (10 -4 ) was used as appropriate after dilution range finding test.…”

Section: Microbiological Parameters 281 Total Heterotrophic Bacterimentioning

confidence: 99%

Enhanced Biodegradation of Degreaser Using Pseudomonas and Bacillus Species in Fresh Water Ecosystem

Nrior

Otuogha

2019

CJAST

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Aim: The aim of this study is to enhance the biodegradation of degreasers used in upstream sectors of Nigeria Petroleum Industry using bio-augmenting organisms such as: Pseudomonas and Bacillus species in freshwater Ecosystem. Study Design: This study employs experimental designs, Randomized Block Design treatment set up, statistical analysis of data and interpretation. Place and Duration of Study: Freshwater sample for this research was collected from Asarama Andoni, in Rivers State, Nigeria. The study lasted for six months. Methodology: The experimental set-up was carried in 500 ml conical flask with two species of bacteria, two types of degreaser and fresh water sample giving a total of 8 set-up including controls. The Pseudomonas and Bacillus species used in this study were isolated from the freshwater ecosystem and identified using standard microbiological methods. The bioremediation potential of the respective test organisms were monitored at ambient temperature 28±0.2°C for 28 days at a constant interval of 7 days using the following Physiochemical parameter; Total dissolved Solid, Hydrogen concentrations ions and Total Hydrocarbon Content. While the following Microbiological parameters; Total heterotrophic Bacteria, Total Heterotrophic Fungi, Hydrocarbon Utilizing Bacteria, and Hydrocarbon Utilizing Fungi were monitored. Results: The percentage of degradability of the respective set-ups ranged from Control (Rigwash) (3.29%) < Pseudomonas sp. + Rigwash (27.56%) < Pseudomonas + Bacillus + Rigwash< (31.57%), Bacillus sp.+ Rigwash (37.57%) Control 2 (Aquabreak) (9.45%) < Pseudomonas sp.+ Aquabreak (26.77%) < Pseudomonas + Bacillus + Aquabreak (31.32%)< Bacillus sp.+ Aquabreak (32.46%). Overall evaluation revealed that Bacillus sp. had a higher biodegradation potential on both degreaser (Rigwash and Aquabreak) in freshwater than Pseudomonas sp. Five species of bacteria: Escherichia coli, Micrococcus, Citrobacter, Bacillus, and Pseudomonas species and four fungal species: Penicillium, Mucor, Aspergillus and Rhizopus species were isolated and identified as hydrocarbon utilizing bacteria and fungi organisms respectively. Conclusion: The results revealed that Bacillus species have more degradability potential than Pseudomonas species for both Aquabreak and Rigwash. These results also indicated the low biodegradation potential of Rigwash in fresh Ecosystem.

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Toxicity of bitumen on soil fertility bacteria - Nitrobacter and Nitrosomonas from Tekulu Waterside, Rivers State, Nigeria

Chidi Juliet Nwankwo,

Lucky Obukowho Odokuma,

Chimezie Jason Ogugbue

2023

GSC Adv. Res. Rev.

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Toxicity of bitumen on Nitrosomonas and Nitrobacter from Tekulu Waterside, Bodo in Gokana Local government area of Ogoni, Rivers State, Nigeria was carried out using standard analytical procedures. The density, API and flash point of the bitumen used in this study at 15°C was 0.9898 g/cm3, API at 60°F value of 11.46 and >200°C respectively. The total TPH and PAH constituent of the bitumen was 20,549.77 mg/L and 924.09 mg/L respectively. Analysis of the effect of different concentration (50 mg/L, 250 mg/L, 500 mg/L, 750 mg/L and 1000 mg/L) of bitumen on Nitrosomonas sp. over a period (24hrs, 48hrs. 72hrs and 96hrs) revealed that the mortality of the organism increased as the concentration of the toxicant. The mortality was also affected by the duration of exposure. The probit analysis on the lethal concentration (LC10, LC20 and LC50) of the bitumen on Nitrosomonas sp. was 223.32 mg/L, 369.682 mg/L and 969.622 mg/L respectively and Nitrobacter sp. 587.837 mg/L, 765.74 mg/L and 1269.841 mg/L respectively, when exposed for 96 hours revealed a varying mortality status. The LC50 of the bitumen on Nitrosomonas sp. peaked at 48hrs. The study shows that bitumen was more toxic to Nitrosomonas than Nitrobacter sp. based on the findings of the study, it is recommended that test organisms (Nitrosomonas sp. and Nitrobacter sp.) could serve as a potential tool for eco-toxicological assay and pollution biomarker.

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Comparative Ecotoxicological Analyses of Spent Phone Batteries on Pseudomonas sp. in Tri Aquatic Environment

Cited by 4 publications

References 0 publications

Enhanced Biodegradation and Release Pattern of Heavy Metal from Spent Laptop Batteries using Pseudomonas and Bacillus Species in Freshwater

Enhanced Biodegradation and Release Pattern of Heavy Metal from Spent Laptop Batteries using Pseudomonas and Bacillus Species in Freshwater

Enhanced Biodegradation of Degreaser Using Pseudomonas and Bacillus Species in Fresh Water Ecosystem

Toxicity of bitumen on soil fertility bacteria - Nitrobacter and Nitrosomonas from Tekulu Waterside, Rivers State, Nigeria

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