Comparative Effect of Commercially Available Nanoparticles on Soil Bacterial Community and “Botrytis fabae” Caused Brown Spot: In vitro and in vivo Experiment

Abstract

This study revealed the possible effects of various levels of silver nanoparticle (AgNP) application on plant diseases and soil microbial diversity. It investigated the comparison between the application of AgNPs and two commercial nanoproducts (Zn and FeNPs) on the rhizobacterial population and Botrytis fabae. Two experiments were conducted. The first studied the influence of 13 AgNP concentration on soil bacterial diversity besides two other commercial nanoparticles, ZnNPs (2,000 ppm) and FeNPs (2,500 ppm), used for comparison and application on onion seedlings. The second experiment was designed to determine the antifungal activity of previous AgNP concentrations (150, 200, 250, 300, 400, and 500 ppm) against B. fabae, tested using commercial fungicide as control. The results obtained from both experiments revealed the positive impact of AgNPs on the microbial community, representing a decrease in both the soil microbial biomass and the growth of brown spot disease, affecting microbial community composition, including bacteria, fungi, and biological varieties. In contrast, the two commercial products displayed lower effects compared to AgNPs. This result clearly showed that the AgNPs strongly inhibited the plant pathogen B. fabae growth and development, decreasing the number of bacteria (cfu/ml) and reducing the rhizosphere. Using AgNPs as an antimicrobial agent in the agricultural domain is recommended.

Role of New Nanosized Feed Additives as Inhibitors of Certain Gram-Positive and Gram-Negative Bacteria and as Anti-Mycotoxigenic Agents

“…This work is licensed under the Creative Commons Attribution 4.0 International License study advised he use of AgNPs as an antibacterial agent in the agricultural sector. 32 As shown in Tables 7 and 8, our results revealed that the tested treatments were more effective at reducing or inhibiting aflatoxin production than reducing A. flavus growth. Our findings are consistent with those of , 85 who reported that the inhibitory effect on fungal growth was not directly related to mycotoxin inhibition.…”

“…31 The antibacterial properties of silver have been known for more than 100 years, and it is used in treatments. 32 Copper nanoparticles (CuNPs) can interact with the organelles of microorganisms and with thiol groups in enzymes and proteins, 29,33 which leads to the accumulation of copper in vacuoles and cell walls as granules and inhibits microorganism growth. 34 CuNPs have greater bactericidal activity due to their larger surface area, which results in synergistic effects that exert antibacterial activity.…”

Biofortification of maize growth, productivity and quality using nano-silver, silicon and zinc particles with different irrigation intervals

“…This has led to a new era of using nanoparticles to enhance agricultural production, but their potential harmful effects on the environment and vegetation should not be ignored (Iqbal et al, 2020;Abdelghany et al, 2022). Silver nanoparticles (AgNPs) have a lot of potential in agriculture, especially when it comes to increasing the pace and development of diploid and triploid seeds (Khafaga et al, 2022;Sabra et al, 2022;Abdelsalam et al, 2022a). Capping phytochemicals in green nanoparticle manufacturing has a positive impact on agriculture.…”