Introduction: The biologically oriented preparation technique (BOPT) is a conservative tooth preparation method with no defined finish lines. Studies comparing the fracture resistance of endodontically treated teeth (ETT) prepared using BOPT or horizontal finish lines are lacking. The primary objective of this study was to compare the fracture resistance of ETT prepared using BOPT, chamfer finish line, or shoulder finish line and restored with monolithic zirconia (MZ) crowns. The secondary objective was to determine the modes of fracture of ETT with different finish lines.Methods: Forty-five maxillary premolar teeth were endodontically treated and randomly allotted to three tooth preparation methods: Group 1, BOPT; Group 2, chamfer finish line; and Group 3, shoulder finish line. MZ crowns were milled and cemented on their respective tooth preparations. The fracture resistance was tested using a universal testing machine. Maximum fracture load was recorded in Newtons (N). The fracture modes were classified using Burkey's codes and a newly proposed fracture grading.Results: The highest fracture resistance was seen in samples from Group 1, followed by Group 2, and the least in Group 3. Tukey's post hoc test showed a significant difference between Group 1 and Group 3 (p<0.05) and between Group 2 and Group 3 (p<0.05). No significant differences were found between Group 1 and Group 2 (p>0.05). Code V and Grade 3B fractures were highest in Group 3 samples. Conclusions: Within the limitations of the present study, it can be concluded that ETT prepared with BOPT and chamfer finish lines had the greatest fracture resistance compared to shoulder finish lines. Teeth prepared with shoulder finish lines had more non-restorable fractures compared to BOPT or chamfer preparations.