Comparative effect of sodium butyrate and sodium propionate on proliferation, cell cycle and apoptosis in human breast cancer cells MCF-7

Semaan, Josiane; El-Hakim, Sandy H; Ibrahim, José-Noël; Safi, Rémi; Elnar, Arpiné Ardzivian; Boustany, Charbel El

doi:10.1007/s12282-020-01063-6

Cited by 52 publications

(41 citation statements)

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“…5h, i). Furthermore, in vitro studies showed that SCFA reduced cell proliferation in liver cancer [19], induced apoptosis in lung cancer [20], and did both in breast cancer [21]. Vecchia et al found that acetate and propionate potentiated the anti-cancer effects of butyrate in leukaemic, cervical adenocarcinoma, melanoma, and breast cancer cells [22].…”

Section: Discussionmentioning

confidence: 99%

Association of Bacteroides acidifaciens relative abundance with high-fibre diet-associated radiosensitisation

et al. 2020

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Background: Patients with pelvic malignancies often receive radiosensitising chemotherapy with radiotherapy to improve survival; however, this is at the expense of increased normal tissue toxicity, particularly in elderly patients. Here, we explore if an alternative, low-cost, and non-toxic approach can achieve radiosensitisation in mice transplanted with human bladder cancer cells. Other investigators have shown slower growth of transplanted tumours in mice fed high-fibre diets. We hypothesised that mice fed a high-fibre diet would have improved tumour control following ionising radiation (IR) and that this would be mediated through the gut microbiota. Results: We investigated the effects of four different diets (low-fibre, soluble high-fibre, insoluble high-fibre, and mixed soluble/insoluble high-fibre diets) on tumour growth in immunodeficient mice implanted with human bladder cancer flank xenografts and treated with ionising radiation, simultaneously investigating the composition of their gut microbiomes by 16S rRNA sequencing. A significantly higher relative abundance of Bacteroides acidifaciens was seen in the gut (faecal) microbiome of the soluble high-fibre group, and the soluble high-fibre diet resulted in delayed tumour growth after irradiation compared to the other groups. Within the soluble high-fibre group, responders to irradiation had significantly higher abundance of B. acidifaciens than non-responders. When all mice fed with different diets were pooled, an association was found between the survival time of mice and relative abundance of B. acidifaciens. The gut microbiome in responders was predicted to be enriched for carbohydrate metabolism pathways, and in vitro experiments on the transplanted human bladder cancer cell line suggested a role for microbial-generated short-chain fatty acids and/or other metabolites in the enhanced radiosensitivity of the tumour cells. Conclusions: Soluble high-fibre diets sensitised tumour xenografts to irradiation, and this phenotype was associated with modification of the microbiome and positively correlated with B. acidifaciens abundance. Our findings might be exploitable for improving radiotherapy response in human patients.

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Section: Discussionmentioning

confidence: 99%

Association of Bacteroides acidifaciens relative abundance with high-fibre diet-associated radiosensitisation

et al. 2020

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“…Administration of sodium propionate resulted in inhibition of MCF-7 cellular proliferation in a dose-dependent manner and cell-cycle arrest [85]. Additionally, isobutyrate (a minor SCFA) can exhibit anticarcinogenic effects in colon carcinoma [86].…”

Section: Discussionmentioning

confidence: 99%

Nutritional combinatorial impact on the gut microbiota and plasma short-chain fatty acids levels in the prevention of mammary cancer in Her2/neu estrogen receptor-negative transgenic mice

Sharma

Arora

Stoll

et al. 2020

Preprint

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31Breast cancer is the second leading cause of cancer-related mortality in women. Various 32 nutritional compounds possess anti-carcinogenic properties which may be mediated through their 4 75 an isothiocyanate present in cruciferous vegetables such as BSp, kale, bok choy, cauliflower and 76 cabbage and also has chemopreventive/chemotherapeutic effects against numerous types of 77 cancers via epigenetic mechanisms [11][12][13]. Studies have shown that SFN is a potent inhibitor of 78 histone deacetylase (HDAC), which is an enzyme that modulates epigenetic machinery by removal 79 of an acetyl group from histone residues. Studies have shown that SFN induced G1/S arrest, led to 80 down-regulation of SEI-1 and cyclin D2, increased levels of p21 and p27 and promoted cellular 81 senescence in breast cancer [14, 15]. (-)-Epigallocatechin-3-gallate (EGCG), a major polyphenol 82 in green tea, induces epigenetic modulations such as inhibition of DNA methytransferases 83 (DNMTs) and has numerous anticarcinogenic properties both in vitro and in vivo against several 84 cancers including breast cancer [16-20]. The anti-tumor mechanisms of GTPs and EGCG involve 85 induction of cell-cycle arrest, mitochondrial-mediated apoptosis, inhibition of IL-6 and induction 86 of tumor necrosis factor-α expression, inhibition of enzymes that regulate the glycolytic process 87 and repression of glucose metabolism [6, 21-23]. 88 Our previous studies have shown that the combination of BSp and GTPs resulted in 89 synergistic inhibition of cellular proliferation, ERα reactivation via regulation of DNMT1 and 90 HDAC1 expression in the ERα (-) breast cancer cell lines MDA-MB-231 and MDA-MB-157, and 91 also resulted in a significant inhibition of tumor development in an ER (-) xenograft mouse model 92 [24]. Additionally, these combined dietary components induced cellular apoptosis and cell cycle 93 arrest in the transformed breast cancer SHR cells (normal human mammary epithelial cells 94 transfected with SV40, hTERT and H-Ras genes), and led to genome-wide epigenetic alterations. 95 This combination treatment administered in a breast cancer xenograft mouse model also resulted 96 in significant inhibition of tumor growth when compared with singly administrated compounds 97 [25]. In addition, our recent study reported that the prenatal or maternal consumption of BSp has 5 98 more protective effects on tumor development than postnatal or adulthood administration of BSp 99 in SV40 transgenic and Her2/neu transgenic mouse models [26]. 100 The human gastrointestinal tract harbors trillions of microorganisms (≥10 14 ) that are 101 reported to be at an approximate ratio of 1:1 with the human cells [27]. The gut microbiota plays 102 an important role in regulation of human metabolic and physiological functions by production of 103 crucial metabolites such as short chain fatty acids (SCFAs) [28, 29]. SCFAs are produced from 104 the fermentation of non-digestible carbohydrates by gut microbiota and the major SCFAs include 105 butyrate, acetate and propionate ...

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“…Several studies have shown the antitumor effects of SCFAs with a major participation of butyrate. For example, butyrate exhibits anti-tumorigenic functions by inhibiting proliferation or inducing apoptosis in a variety of human CRC cells at physiological concentrations (Fung et al, 2012a,b) and several other cancer cells, such as breast cancer cells (Semaan et al, 2020), bladder cancer cells (Wang et al, 2020), and lung cancer cells (Xiao et al, 2020). This function has been attributed to butyrate's ability to alter gene transcription by inhibiting histone deacetylase activity (Fung et al, 2012a); however, the participation of FFA receptors has also been postulated.…”

Section: Role Of Scfa and Their Receptors In Colorectal Cancermentioning

confidence: 99%

Participation of Short-Chain Fatty Acids and Their Receptors in Gut Inflammation and Colon Cancer

et al. 2021

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Short-chain fatty acids (SCFAs) are the main metabolites produced by the bacterial fermentation of dietary fiber, and they play a critical role in the maintenance of intestinal health. SCFAs are also essential for modulating different processes, and they have anti-inflammatory properties and immunomodulatory effects. As the inflammatory process predisposes the development of cancer and promotes all stages of tumorigenesis, an antitumor effect has also been associated with SCFAs. This is strongly supported by epidemiological studies showing that a diet rich in fiber is linked to a reduced risk of colon cancer and has significant clinical benefits in patients with inflammatory bowel disease (IBD). SCFAs may signal through the metabolite-sensing G protein-coupled receptors free fatty acid receptor 3 [FFAR3 or G protein-coupled receptor 41 (GPR41)], FFAR2 (GPR43), and GPR109A (also known as hydroxycarboxylic acid receptor 2 or HCAR2) expressed in the gut epithelium and immune cells. This review summarizes the existing knowledge regarding the SCFA-mediated suppression of inflammation and carcinogenesis in IBD and colon cancer.

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Comparative effect of sodium butyrate and sodium propionate on proliferation, cell cycle and apoptosis in human breast cancer cells MCF-7

Cited by 52 publications

References 34 publications

Association of Bacteroides acidifaciens relative abundance with high-fibre diet-associated radiosensitisation

Association of Bacteroides acidifaciens relative abundance with high-fibre diet-associated radiosensitisation

Nutritional combinatorial impact on the gut microbiota and plasma short-chain fatty acids levels in the prevention of mammary cancer in Her2/neu estrogen receptor-negative transgenic mice

Participation of Short-Chain Fatty Acids and Their Receptors in Gut Inflammation and Colon Cancer

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