Comparative Effect of Three Different Exercise Intensities in Combination with Diazoxide on Contraction Capacity and Oxidative Stress of Skeletal Muscle in Obese Rats

Abstract

Obesity is a chronic disease that impairs skeletal muscle function, affects the ability to contract, and promotes the development of fatigue. For this reason, the study of treatments that seek to reduce the harmful effects of obesity on muscle tissue has been deepened. Diazoxide treatment and various exercise protocols have been proposed to protect skeletal muscle against oxidative stress and its effects. However, the intensity and duration of exercise combined with diazoxide that would obtain the best results for improving skeletal muscle function in obese rats is unknown. To this end, this study evaluated the effects of three different exercise intensities combined with diazoxide on contraction capacity, resistance to fatigue, markers of oxidative stress, lipid peroxidation, ROS, and glutathione redox status of skeletal muscle. The results showed that treatments with diazoxide and exercise at different intensities improved muscle contraction capacity by reducing oxidative stress during obesity, with the best results being obtained with low-intensity exercise in combination with diazoxide. Therefore, these results suggest that diazoxide and low-intensity exercise improve muscle function during obesity by decreasing oxidative stress with the same efficiency as a moderate-intensity exercise protocol.

Diazoxide choline extended‐release tablet in people with Prader‐Willi syndrome: results from long‐term open‐label study

“…There were broad-ranging impacts on PWS-associated behaviors, which were realized at 52 weeks as measured using the PWSP and to increase the benefit of exercise on muscle strength, resistance to fatigue, and muscle reactive oxygen species and redox status [17,18].…”

“…Consequently, there is an expectation of accumulation of muscle mass, weight, and fat mass over the course of 52 weeks if they are characteristically developing individuals with PWS, although prior studies of people with PWS have indicated that DCCR leads to increases in lean mass and decreases in fat mass versus placebo [13]. Diazoxide use in obese animal models has been shown to increase the benefit of exercise on muscle strength, resistance to fatigue, and muscle reactive oxygen species and redox status [17, 18].…”