Comparative effects of 3,4-dimethylpyrazole phosphate (DMPP) and dicyandiamide (DCD) on ammonia-oxidizing bacteria and archaea in a vegetable soil

Chen, Qiuhui; Qi, Lingyu; Bi, Qincheng; Dai, Peibin; Sun, Dong; Sun, Chengliang; Liu, Wenjing; Lu, Lingli; Ni, Wuzhong; Lin, Xianyong

doi:10.1007/s00253-014-6026-7

Cited by 84 publications

(43 citation statements)

References 56 publications

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“…T-RFLP analysis showed that urea addition had a significant impact on the community structure of AOB compared to other treatments (Fig. 4a), which was consistent with previous findings (Jia and Conrad 2009;Chen et al 2015). However, the AOB community composition between the urea + DCD and urea + C 2 H 2 treatments was similar in our study.…”

Section: Aoa-otu7 (256bp)supporting

confidence: 92%

“…3a), which was in agreement with previous studies in a large number of agricultural soils (Wu et al 2011;Habteselassie et al 2013;Chen et al 2015). It has been reported that AOB growth is favored by high-N soil conditions (Jia and Conrad 2009;Verhamme et al 2011;Taylor et al 2012), whereas AOA growth is not affected (Di et al 2009(Di et al , 2010a.…”

Section: Nitrosospira Nitrososmonassupporting

confidence: 92%

“…These compounds inhibit nitrification by deactivating ammonia monooxygenase (AMO) and blocking the growth of AOB and AOA (Di et al 2010a;Hink et al 2016). DCD or C 2 H 2 was reported to be more effective in inhibiting AOA compared with AOB in low-pH soils (Gubry-Rangin et al 2010;Zhang et al 2012), and vice verse in nitrogen-rich soils (Di et al 2009;Jia and Conrad 2009;Dai et al 2013;Chen et al 2015). Moreover, by comparing the inhibitory effects of different NIs on the growth of ammonia oxidizers using pure cultures, DCD exhibited a stronger inhibitory effect on AOB than AOA, while nitrapyrin dramatically inhibited the growth of AOA (Shen et al 2013).…”

Section: Introductionmentioning

confidence: 97%

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Effects of dicyandiamide and acetylene on N2O emissions and ammonia oxidizers in a fluvo-aquic soil applied with urea

Wang

Zhang

Shen

et al. 2016

Environ Sci Pollut Res

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Ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) are crucial for N 2 O emission as they carry out the key step of nitrification. Dicyandiamide (DCD) and acetylene (C 2 H 2 ) are typical nitrification inhibitors (NIs), while the comparative effects of these NIs on N 2 O production and ammonia oxidizers' (AOB and AOA) growth are unclear. Four treatments including a control, urea, urea + DCD, and urea + C 2 H 2 were set up to investigate their effect of inhibiting soil nitrification, nitrification-related N 2 O emission as well as the growth of ammonia oxidizers with a fluvo-aquic soil using microcosms for 28 days. N 2 O emission and net nitrification rate increased after the application of urea, but were significantly restrained in urea + NI treatments, while C 2 H 2 was more effective in reducing N 2 O emission and nitrification rate than DCD. The abundance of AOB, which was significantly correlated with N 2 O emission and net nitrification rate, was more inhibited by C 2 H 2 than DCD. Furthermore, the application of urea in all the soils had little impact on the AOA community, while obvious shifts of AOB community structure were found compared with the control. All AOB sequences fell within Nitrosospira cluster 3, and the AOA community was clustered to group 1.1b. Collectively, it indicated that application of urea combined with NIs (DCD or C 2 H 2 ) could potentially alter N 2 O emission, mainly through regulating the growth of AOB but not AOA in this fluvo-aquic soil.

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Section: Aoa-otu7 (256bp)supporting

confidence: 92%

Section: Nitrosospira Nitrososmonassupporting

confidence: 92%

Section: Introductionmentioning

confidence: 97%

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Effects of dicyandiamide and acetylene on N2O emissions and ammonia oxidizers in a fluvo-aquic soil applied with urea

Wang

Zhang

Shen

et al. 2016

Environ Sci Pollut Res

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“…AOA did not respond to the application of NH 4 + in the alluvial soil, while it was inhibited with respect to the control in the red soil, thus confirming what it was obtained in agricultural soils (Wu et al 2011;Ai et al 2013;Habteselassie et al 2013;Di et al 2014Di et al , 2014Chen et al 2015). Generally, AOB growth is favored by high NH 4 + -N soil conditions (Jia and Conrad 2009;Verhamme et al 2011;Taylor et al 2012), whereas AOA growth is not affected or suppressed (Di et al 2009(Di et al , 2010Di and Cameron 2011;Di et al 2014).…”

Section: Discussionsupporting

confidence: 68%

Nitrogen fertiliser-induced changes in N2O emissions are attributed more to ammonia-oxidising bacteria rather than archaea as revealed using 1-octyne and acetylene inhibitors in two arable soils

et al. 2016

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Nitrification is believed to be one of the major sources of N 2 O production emitted from soil. Previous studies showed that both ammonia-oxidising bacteria (AOB) and archaea (AOA) can produce N 2 O via nitrification but their relative contributions are still poorly defined. Here, we used acetylene, an inhibitor of AOB and AOA ammonia monooxygenase (AMO), and 1-octyne, a selective inhibitor that specifically inhibits AOB AMO, to investigate how AOB versus AOA contribute to N 2 O emissions in two distinct arable soils. Soil amended with ammonium (NH 4 + ) increased N 2 O emissions to a greater extent than nitrate (NO 3 − ), and acetylene had a greater impact on N 2 O emissions in NH 4 + -treated soils than that in NO 3 − -amended soils, which indicated that nitrification was the dominant N 2 O emitting process in these two arable soils. In the alluvial and red soil, the percentage of evolved N 2 O after application of NH 4 + by AOB were 70.5~78.1 % and 18.7~19.7 % by AOA, respectively. Quantitative PCR revealed that NH 4 + addition stimulated AOB growth, and the growth could be significantly inhibited by acetylene or 1-octyne in the two soils. The stimulation of N 2 O emissions by NH 4 + and the relative suppression by inhibitors paralleled fluctuations in the AOB growth. In addition, cumulative N 2 O emissions were not correlated with AOA abundance in the two soils. Our results revealed that AOB could contribute more to soil N 2 O production than AOA in the NH 4 + -amended arable soils.

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“…Furthermore, the rising costs of synthetic N fertilizers have strengthened farmers' commercial incentive to reduce N fertilization and as a consequence N losses (Ernfors et al 2014). Nitrification is a critical component in the N cycle, which causes N losses in the form of nitrate (NO 3 − ) leaching and nitrous oxide (N 2 O) emissions from agricultural systems (Wakelin et al 2014;Chen et al 2015). Nitrate leaching could trigger waterway eutrophication and drinking water contamination (Philippot et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Effects of 3,4-dimethylpyrazole phosphate (DMPP) on nitrification and the abundance and community composition of soil ammonia oxidizers in three land uses

Shi

et al. 2016

Biol Fertil Soils

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The application of the nitrification inhibitor, 3,4-dimethylpyrazole-phosphate (DMPP), is considered as an effective strategy to mitigate agricultural nitrogen loss. However, the inhibitory effect of DMPP on nitrification is variable and the importance of the soil microbial community composition to the variability is poorly understood. In this study, nine soils were collected across three land uses to investigate the impact of DMPP on nitrification and associated dynamics of ammonia oxidizers in a 28-day microcosm incubation. The results showed that the efficacy of DMPP at inhibiting net nitrification rates varied highly from no effect to 63.6 % during the first week of incubation. The abundance of ammonia-oxidizing bacteria (AOB), rather than ammoniaoxidizing archaea (AOA), was significantly correlated with nitrate concentrations across three land uses and significantly inhibited by DMPP addition. DMPP had higher efficacy in neutral and alkaline wheat and vegetable soils, compared with pasture soils. Canonical correspondence analysis suggested that soil pH was the most influential factor explaining the community composition of AOB and AOA in the collected soils. However, neither ammonium nitrate nor DMPP addition had a significant effect on the community composition of AOB or AOA during the incubation indicated by non-metric multidimensional scaling ordination. Taken together, our findings indicated that DMPP slowed nitrification by inhibiting the growth of AOB, and DMPP application affected the abundance of AOB more than the ammonia oxidizer community composition.

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Comparative effects of 3,4-dimethylpyrazole phosphate (DMPP) and dicyandiamide (DCD) on ammonia-oxidizing bacteria and archaea in a vegetable soil

Cited by 84 publications

References 56 publications

Effects of dicyandiamide and acetylene on N2O emissions and ammonia oxidizers in a fluvo-aquic soil applied with urea

Effects of dicyandiamide and acetylene on N2O emissions and ammonia oxidizers in a fluvo-aquic soil applied with urea

Nitrogen fertiliser-induced changes in N2O emissions are attributed more to ammonia-oxidising bacteria rather than archaea as revealed using 1-octyne and acetylene inhibitors in two arable soils

Effects of 3,4-dimethylpyrazole phosphate (DMPP) on nitrification and the abundance and community composition of soil ammonia oxidizers in three land uses

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