Comparative Effects of Coated Compound and Mono-component Proteases on Growth Performance and Nutritional Efficiency in Broiler Diets

Abstract

Protease as feed additive is being used in poultry production as a partial replacement for protein sources for cost efficiency and reducing nitrogen excretion. However, diverse proteases may yield different responses under field conditions. A pellet diet study was conducted in Cobb broilers to assess the impact of coated compound (CC) and mono-component (MC) proteases with 5% replacement of digestible amino acids and 0.9% crude protein. Birds fed positive control diet had a better growth than those fed negative control diet, regardless of enzyme supplementation. However, CC protease had shown feed conversion ratio (FCR) like control in a reformulated diet, whereas negative control and MC protease missed to gain the feed conversion. In measures of nutritional efficiency, like energy efficiency, protein efficiency and amino acids efficiency (lysine and methionine), the CC protease proved to be better than MC protease. In terms of European efficiency factor (EEF), control and CC protease elicited a closer response, whereas the other two groups showed a drop. In this study, CC protease allowed partial substitution of digestible amino acids and crude protein, while maintaining feed efficiency and animal performance. It could be concluded that incorporating CC proteases is an efficient choice to maximize the utilization feed material resources and efficiency in animal protein production.

Multiprotease improves amino acid release in vitro, energy, and nutrient utilization in broilers fed diets varying in crude protein levels

“…Six treatments resulting from a 3 × 2 factorial arrangement of 3 dietary CP levels (17, 19, and 21%) and 2 levels of exogenous multiprotease (KEMZYME TM Protease, Kemin Industries, Des Moines, IA) supplementation (0 and 2,400 U/kg) were evaluated in a completely randomized design with seven replicates per treatment. The 2,400 U/kg equates to 300 g/t feed and is in line with the use of this protease in other studies ( Chandrasekar et al, 2017 ; De Leon et al, 2021 ; Wealleans et al, 2023 ). The multiprotease includes a combination of acid (pepsin type protease), neutral (metallo endo-peptidase), and alkaline (serine endopeptidase), proteases, produced by Aspergillus niger, Bacillus subtilis, and Bacillus licheniformis , respectively ( Lim et al, 2014 ).…”

Evaluation of lipid matrix microencapsulation for intestinal delivery of thymol in weaned pigs

“…The benefits of encapsulation are to 1) improve the storage stability of feed additives; 2) protect feed additives during feed processing, including mixing, conditioning, and pelleting; 3) mask unpleasant odor that can decrease feed intake; 4) improve the ease of handling of liquid feed additives (e.g., EO) by changing liquid to solid state; 5) slowly release bioactive compounds along the gut of animals; and 6) reduce the effective dosage of bioactive compounds that have high cost and environmental issues. A broad range of bioactive compounds, such as probiotics ( Liu et al, 2016 ), EO ( Omonijo et al, 2018a ), zinc oxide ( Xie et al, 2011 ), OA ( Grilli et al, 2010 ), bacteriophages ( Huff et al, 2013 ), and enzymes ( Chandrasekar et al, 2017 ) have been encapsulated for improving animal health.…”