Comparative effects of different dietary levels of cellulose and fructooligosaccharides on fermentative processes in the caecum of rats

Abstract

Forty eight male Wistar rats were divided into 6 groups and fed over 4 weeks with diets containing 2.5, 5 or 10% addition of potentially inert carbohydrate cellulose (CEL) or easily fermentable fructooligosaccharides (FOS). In rats fed on CEL diets no substantial changes in the pH, caecal mass, as well as protein, ammonia and short-chain fatty acids (SCFA) concentration in the caecal digesta were observed; almost all microbial enzymes activity also was not modifi ed, except the activity of β-glucuronidase which decreased considerably upon higher doses of CEL (5 and 10%). Lower FOS supplementation (2.5 and 5%) led to desirable changes typical for dietary fructans (increased SCFA concentration and acidifi cation of digesta), while the 10% addition of FOS had some unfavourable effects such as decreased SCFA concentration, especially butyrate, and excessive enlargement of the caecum (tissue and digesta). In conclusion, 10% addition of FOS seems to be an overdose, while CEL can be recognized as a good negative control in experiments with non-digestible saccharides, however, its infl uence on microfl ora activity should be considered during the interpretation of the obtained results.

Effects of inulin supplemented to cellulose-free or cellulose-rich diets on caecal environment and biochemical blood parameters in rats

“…The results of our experiment showing that neither cellulose (CEL) nor inulin (I) supplementation significantly affected final body weight of rats are in agreement with the observations reported in the earlier study, where diets were supplemented with 2.5, 5 and 10% of cellulose or fructooligosaccharides (FOS) (Jurgoński et al, 2008). …”

“…Addition of cellulose did not influence total SCFAs pool. The latter observation is in agreement with results demonstrating that CEL affects caecal fermentative processes only to small extent; they suggested that CEL-diet can be applied as a suitable negative control while studying the properties of potentially prebiotic carbohydrates (Jurgoński et al, 2008).…”

“…Many studies on diets enriched in non-digestible saccharides have demonstrated that SCFA pool size, which takes into account the amount of digesta in the intestine, is a parameter reflecting more accurately the intensity of the bacterial fermentative processes, than the SCFA concentration (Berggren et al, 1993;Jurgoński et al, 2008). The increased concentration of butyrate after prebiotic treatment is of particular significance.…”

“…Some previous reports claimed that cellulose is relatively inert in the rat large intestine and does not significantly influence microbial proliferation (Reshef et al, 1990;Johansen and Bach Knudsen, 1994). On the other hand, recent studies have shown that insoluble dietary fibres such as cellulose are not completely inert and their breakdown in the lower gastrointestinal tract should be taken into account in the interpretation of nutritional experimental data (Takahashi et al, 2005;Jurgoński et al, 2008).…”

Pengaruh Penggunaan Sekam Padi Dalam Ransum Berbasis Limbah Pangan Hotel Kering Terhadap Pertumbuhan Dan Karkas Babi

“…Penurunan tebal lemak punggung dengan peningkatan sekam padi dalam ransum terjadi karena sekam padi mampu mengikat lemak ransum untuk diekskresikan bersama dengan feses. Hal ini sesuai dengan pendapat Jurkonski et al (2008) yang menyatakan bahwa partikel serat umumnya memiliki gugus penukar ion kation yang memiliki kemampuan seperti matriks spons untuk mengikat partikel lemak dengan ikatan intraluminal. Hasil ini sesuai dengan yang pernah dilaporkan oleh Wilfart et al (2007), bahwa peningkatan konsentrasi serat dalam ransum menyebabkan peningkatan kandungan ekstrak ether feses ternak babi.…”