Grasses from lowland ecosystems in flooded savannahs are useful to feed extensive grazing animals; however, scarce information about its agronomic and fermentation characteristics exists. This study aims to determine the chemical composition and fermentation parameters of native grasses from the floodplain lowlands ecosystem in the Colombian Orinoquia. Three native grasses (Leersia hexandra, Acroceras zizanioides and Hymenachne amplexicaulis) and a “control” grass (introduced Urochloa arrecta—Tanner grass) were sown and sampled at 30, 40 and 50 days of age. On each sampling date, biomass production in a 1 m2 frame was estimated, and the chemical composition and fermentation parameters were analyzed using near-infrared spectroscopy and the in vitro gas production technique, respectively. Data were analyzed using a mixed model for repeated measures and the least significant difference (LSD) was used for mean differentiation (p < 0.05). The grasses’ nutritional characteristics varied as follows: dry matter (DM, 0.7–2.0 ton/ha), crude protein (CP, 6.1–12.2%), neutral detergent fiber (NDF, 56.6–69.6%), ash (5.8–15.8%) and dry matter digestibility (DMD) between 20.8 and 60.6% from 12 to 48 h of fermentation. Native plants such as L. hexandra and A. zizanioides presented higher biomass production, CP, ash, cellulose, and Ca levels than the control plant. During the experimental period (30 to 50 days), the grasses did not present significant nutrient availability changes. In terms of fermentation characteristics, L. hexandra increased ammonia concentrations and total volatile fatty acids (TVFA) and butyric acid. This latter effect was also observed in A. zizanioides grass. L. hexandra and A. zizanioides grasses constitute a valuable alternative forage resource during the flooding times of the studied ecosystem.