Comparative Effects of Fumonisins on Sphingolipid Metabolism and Toxicity in Ducks and Turkeys

Benlasher, Emad; XiuYu, Geng; Nguyen, N.T.; Tardieu, Didier; Bailly, Jean‐Denis; Auvergne, Alain; Guerre, Philippe

doi:10.1637/9853-071911-reg.1

Cited by 16 publications

(16 citation statements)

References 38 publications

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“…To evaluate the efficacy of the commercial application of FumD (FUM zyme ) in vivo , a feeding trial with turkeys was performed. Since turkeys are more sensitive to FB 1 exposure than poultry (summarized by Benlasher et al [ 40 ]), this species was chosen as the avian model for our experiment. Turkeys were fed basal diet (CON), fumonisin-contaminated diet (15 mg/kg FB 1 +FB 2 ; FB), or a fumonisin-contaminated diet supplemented with FUM zyme (15 mg/kg FB 1 +FB 2 , 15 U/kg FUM zyme ; FB+FUM zyme ) for 14 days ad libitum .…”

Section: Resultsmentioning

confidence: 99%

“…Yet, effects of fumonisins on performance parameters or liver weights were found to become evident only at high levels of dietary FB 1 (50–325 mg/kg FB 1 upwards) [ 48 , 49 , 50 ]. On the contrary, disruption of the sphingolipid metabolism was observed after chronic exposure to comparable low doses of FB 1 [ 40 , 51 ]. Since an increase in the Sa/So ratio precedes other signs of toxicity, this parameter was suggested as a sensitive fumonisin biomarker in turkeys [ 50 , 51 ].…”

Section: Resultsmentioning

confidence: 99%

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Gastrointestinal Degradation of Fumonisin B1 by Carboxylesterase FumD Prevents Fumonisin Induced Alteration of Sphingolipid Metabolism in Turkey and Swine

Masching¹,

Naehrer²,

Schwartz-Zimmermann

et al. 2016

Toxins

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The mycotoxin fumonisin B1 (FB1) is a frequent contaminant of feed and causes various adverse health effects in domestic animals. Hence, effective strategies are needed to prevent the impact of fumonisins on livestock productivity. Here we evaluated the capability of the fumonisin carboxylesterase FumD to degrade FB1 to its less toxic metabolite hydrolyzed FB1 (HFB1) in the gastrointestinal tract of turkeys and pigs. First, an ex vivo pig model was used to examine the activity of FumD under digestive conditions. Within 2 h of incubation with FumD, FB1 was completely degraded to HFB1 in the duodenum and jejunum, respectively. To test the efficacy of the commercial application of FumD (FUMzyme) in vivo, female turkeys (n = 5) received either basal feed (CON), fumonisin-contaminated feed (15 mg/kg FB1+FB2; FB) or fumonisin-contaminated feed supplemented with FUMzyme (15 U/kg; FB+FUMzyme) for 14 days ad libitum. Addition of FUMzyme resulted in significantly decreased levels of FB1 in excreta, whereas HFB1 concentrations were significantly increased. Compared to the FB group (0.24 ± 0.02), the mean serum sphinganine-to-sphingosine (Sa/So) ratio was significantly reduced in the FB+FUMzyme group (0.19 ± 0.02), thus resembling values of the CON group (0.16 ± 0.02). Similarly, exposure of piglets (n = 10) to 2 mg/kg FB1+FB2 for 42 days caused significantly elevated serum Sa/So ratios (0.39 ± 0.15) compared to the CON group (0.14 ± 0.01). Supplementation with FUMzyme (60 U/kg) resulted in gastrointestinal degradation of FB1 and unaffected Sa/So ratios (0.16 ± 0.02). Thus, the carboxylesterase FumD represents an effective strategy to detoxify FB1 in the digestive tract of turkeys and pigs.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Gastrointestinal Degradation of Fumonisin B1 by Carboxylesterase FumD Prevents Fumonisin Induced Alteration of Sphingolipid Metabolism in Turkey and Swine

Masching¹,

Naehrer²,

Schwartz-Zimmermann

et al. 2016

Toxins

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“…In our study, we observed a non-significant change in feed intake and feed conversion in the broilers treated with FB1 alone compared to control. Previous studies have reported a decrease in feed intake and feed conversion when broilers, ducks, or turkeys were ingested with feed contaminated by FB1 ( Tessari et al, 2006 ; Benlasher et al, 2012 ). Further, our study showed no significant variations of body weight in broilers of treatment groups but health complications such as diarrhea and dysentery was observed in broilers fed with 200 mg FB1 per Kg feed.…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus plantarum MYS6 Ameliorates Fumonisin B1-Induced Hepatorenal Damage in Broilers

et al. 2017

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Fumonisin B1 (FB1), a mycotoxin produced by Fusarium species is a predominant Group 2B carcinogen occurring in maize and maize-based poultry feeds. It is shown to be nephrotoxic, hepatotoxic, neurotoxic, and immunosuppressing in animals. In this study, we report the ameliorating effects of a probiotic strain, Lactobacillus plantarum MYS6 on FB1-induced toxicity and oxidative damage in broilers. A 6-week dietary experiment consisting of 48 broilers was performed in six treatment groups. Probiotic treatment (109 cells/mL) involved pre-colonization of broilers with L. plantarum MYS6 while co-administration treatment involved supplementation of probiotic and FB1-contaminated diet (200 mg/Kg feed) simultaneously. At the end of the treatment period, growth performance, hematology, serum biochemistry, and markers of oxidative stress in serum and tissue homogenates were evaluated in all the broilers. The histopathological changes in hepatic and renal tissues were further studied. The results demonstrated that administration of L. plantarum MYS6 efficiently improved the feed intake, body weight and feed conversion ratio in broilers. It mitigated the altered levels of hematological indices such as complete blood count, hemoglobin, and hematocrit. Serum parameters such as serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, creatinine, cholesterol, triglycerides, and albumin were significantly restored after administering the probiotic in FB1-intoxicated broilers. Additionally, L. plantarum MYS6 alleviated the levels of oxidative stress markers in serum and tissue homogenate of liver. The histopathological data of liver and kidney further substantiated the overall protection offered by L. plantarum MYS6 against FB1-induced cellular toxicity and organ damage in broilers. Our results indicated that co-administration of probiotic along with the toxin had better effect in detoxification compared to its pre-colonization in broilers. Collectively, our study signifies the protective role of L. plantarum MYS6 in ameliorating the FB1-induced toxicity in the vital organs and subsequent oxidative stress in broilers. The probiotic L. plantarum MYS6 can further be formulated into a functional feed owing to its anti-fumonisin attributes and role in mitigating FB1-induced hepatorenal damage.

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“…As a point of comparison, swine develops lethal pulmonary edema within 4–7 days when fed FB containing feed or culture material at concentrations of ≥ 92 mg/kg [ 13 ]. Like mammals, poultry species also exhibit differential sensitivity, with lower concentrations of FB being more detrimental to turkeys and ducks than to chickens [ 14 , 15 ].…”

Section: Discussionmentioning

confidence: 99%

Dose-Dependent Effects on Sphingoid Bases and Cytokines in Chickens Fed Diets Prepared with Fusarium Verticillioides Culture Material Containing Fumonisins

Grenier

Schwartz-Zimmermann

Caha

et al. 2015

Toxins

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In chickens, the effect of mycotoxins, especially fumonisins (FB), in the gastrointestinal tract (GIT) is not well documented. Thus, this study in broiler chicks determined the effects of consuming diets prepared with Fusarium verticillioides culture material containing FB on intestinal gene expression and on the sphinganine (Sa)/sphingosine (So) ratio (Sa/So; a biomarker of FB effect due to disruption of sphingolipid metabolism). Male broilers were assigned to 6 diets (6 cages/diet; 6 birds/cage) from hatch to 20 days containing 0.4, 5.6, 11.3, 17.5, 47.8, or 104.8 mg FB/kg diet. Exposure to FB altered the Sa/So ratio in all tissues analyzed, albeit to varying extents. Linear dose-responses were observed in the kidney, jejunum and cecum. The liver and the ileum were very sensitive and data fit a cubic and quadratic polynomial model, respectively. Gene expression in the small intestine revealed low but significant upregulations of cytokines involved in the pro-inflammatory, Th1/Th17 and Treg responses, especially at 10 days of age. Interestingly, the cecal tonsils exhibited a biphasic response. Unlike the sphingolipid analysis, the effects seen on gene expression were not dose dependent, even showing more effects when birds were exposed to 11.3 mg FB/kg. In conclusion, this is the first report on the disruption of the sphingolipid metabolism by FB in the GIT of poultry. Further studies are needed to reach conclusions on the biological meaning of the immunomodulation observed in the GIT, but the susceptibility of chickens to intestinal pathogens when exposed to FB, at doses lower than those that would cause overt clinical symptoms, should be addressed.

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Comparative Effects of Fumonisins on Sphingolipid Metabolism and Toxicity in Ducks and Turkeys

Cited by 16 publications

References 38 publications

Gastrointestinal Degradation of Fumonisin B1 by Carboxylesterase FumD Prevents Fumonisin Induced Alteration of Sphingolipid Metabolism in Turkey and Swine

Gastrointestinal Degradation of Fumonisin B1 by Carboxylesterase FumD Prevents Fumonisin Induced Alteration of Sphingolipid Metabolism in Turkey and Swine

Lactobacillus plantarum MYS6 Ameliorates Fumonisin B1-Induced Hepatorenal Damage in Broilers

Dose-Dependent Effects on Sphingoid Bases and Cytokines in Chickens Fed Diets Prepared with Fusarium Verticillioides Culture Material Containing Fumonisins

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