Comparative effects of glycinebetaine on the thermotolerance in codA- and BADH-transgenic tomato plants under high temperature stress

Zhang, Tianpeng; Li, Zhimei; Li, Daxing; Li, Chongyang; Wei, Dandan; Li, Shufen; Liu, Yang; Chen, Tony H. H.; Yang, Xinghong

doi:10.1007/s00299-020-02581-5

Cited by 22 publications

(10 citation statements)

References 59 publications

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“…GB, one of the most prevalent and powerful osmoprotectants in a number of higher plants, is an environmentally healthy, nontoxic and water-resoluble osmolyte [27][28][29][30][31][32][33][34]. Resistance to stress due to various abiotic pressures has been recorded to be associated with GB accumulation [35][36][37][38][39][40][41][42][43][44][45][46][47][48]. GB also protects plants through the stabilization of membranes, proteins and enzymes in addition to its function as an osmoprotectant [28,[49][50][51].…”

Section: Introductionmentioning

confidence: 99%

Glycinebetaine mitigates drought stress-induced oxidative damage in pears

et al. 2021

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Glycinebetaine (GB) is an osmoprotectant found in plants under environmental stresses that incorporates drought and is associated with drought tolerance in several plants, such as the woody pear. However, how GB improves drought tolerance in pears remains unclear. In the current study, we explored the mechanism by which GB enhances drought tolerance of whole pear plants (Pyrus bretschneideri Redh. cv. Suli) supplied with exogenous GB. The results showed that on the sixth day after withholding water, levels of O2·−, H2O2, malonaldehyde (MDA) and electrolyte leakage in the leaves were substantially increased by 143%, 38%, 134% and 155%, respectively. Exogenous GB treatment was substantially reduced O2·−, H2O2, MDA and electrolyte leakage (38%, 24%, 38% and 36%, respectively) in drought-stressed leaves. Furthermore, exogenous GB induced considerably higher antioxidant enzyme activity in dry-stressed leaves than drought-stressed treatment alone on the sixth day after withholding water, such as superoxide dismutase (SOD) (201%) and peroxidase (POD) (127%). In addition, these GB-induced phenomena led to increased endogenous GB levels in the leaves of the GB 100 + drought and GB 500 + drought treatment groups by 30% and 78%, respectively, compared to drought treatment alone. The findings obtained were confirmed by the results of the disconnected leaf tests, in which GB contributed to a substantial increase in SOD activity and parallel dose- and time-based decreases in MDA levels. These results demonstrate that GB-conferred drought resistance in pears may be due in part to minimizing symptoms of oxidative harm incurred in response to drought by the activities of antioxidants and by reducing the build-up of ROS and lipid peroxidation.

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Section: Introductionmentioning

confidence: 99%

Glycinebetaine mitigates drought stress-induced oxidative damage in pears

et al. 2021

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“…Developing transgenic plants for thermotolerance is a cost-effective and efficient biotechnological approach for achieving optimum agricultural production under the changing climate scenario [17]. Genes involved in encoding GB biosynthetic enzymes in different organisms and plants have been cloned to produce transgenic plants overexpressing one or more of these genes to enhance endogenous GB production, improving HS tolerance [67,68] (Table 3). For example, Zhang et al [68] compared the thermotolerance ability of two transgenic tomato lines containing the betaine aldehyde dehydrogenase (BADH) and choline oxidase (COD) genes responsible for GB synthesis.…”

Section: Genetic Engineering For Enhanced Thermotolerancementioning

confidence: 99%

“…Genes involved in encoding GB biosynthetic enzymes in different organisms and plants have been cloned to produce transgenic plants overexpressing one or more of these genes to enhance endogenous GB production, improving HS tolerance [67,68] (Table 3). For example, Zhang et al [68] compared the thermotolerance ability of two transgenic tomato lines containing the betaine aldehyde dehydrogenase (BADH) and choline oxidase (COD) genes responsible for GB synthesis. They observed that codA transgenic plants had higher GB levels, CO 2 assimilation rate, and photosystem II (PSII) photochemical activity and lower accumulation of H 2 O 2 , O 2…”

Section: Genetic Engineering For Enhanced Thermotolerancementioning

confidence: 99%

Role of Glycine Betaine in the Thermotolerance of Plants

2022

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As global warming progresses, agriculture will likely be impacted enormously by the increasing heat stress (HS). Hence, future crops, especially in the southern Mediterranean regions, need thermotolerance to maintain global food security. In this regard, plant scientists are searching for solutions to tackle the yield-declining impacts of HS on crop plants. Glycine betaine (GB) has received considerable attention due to its multiple roles in imparting plant abiotic stress resistance, including to high temperature. Several studies have reported GB as a key osmoprotectant in mediating several plant responses to HS, including growth, protein modifications, photosynthesis, gene expression, and oxidative defense. GB accumulation in plants under HS differs; therefore, engineering genes for GB accumulation in non-accumulating plants is a key strategy for improving HS tolerance. Exogenous application of GB has shown promise for managing HS in plants, suggesting its involvement in protecting plant cells. Even though overexpressing GB in transgenics or exogenously applying it to plants induces tolerance to HS, this phenomenon needs to be unraveled under natural field conditions to design breeding programs and generate highly thermotolerant crops. This review summarizes the current knowledge on GB involvement in plant thermotolerance and discusses knowledge gaps and future research directions for enhancing thermotolerance in economically important crop plants.

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“…According to the newly released sixth assessment report of IPCC ( 2021 ), temperature during the twenty-first century is likely to increase by 1.5°C of warming within just the next two decades, and by 4.5°C, depending on the rate of greenhouse gas emissions. As plants are sedentary organisms, they acclimate to HS by using avoidance mechanisms or programmed cell death (Mittler et al, 2012 ; Singh, 2013 ; Zhang T. et al, 2020 ). Each vegetable crop has temperature threshold for its growth and development; HS will occur beyond the upper threshold for temperature (Wahid et al, 2007 ; Prasad et al, 2008 , 2017 ).…”

Section: Heat Stress and Vegetablesmentioning

confidence: 99%

Physiological and Molecular Approaches for Developing Thermotolerance in Vegetable Crops: A Growth, Yield and Sustenance Perspective

et al. 2022

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Vegetables are a distinct collection of plant-based foods that vary in nutritional diversity and form an important part of the healthy diet of the human being. Besides providing basic nutrition, they have great potential for boosting human health. The balanced consumption of vegetables is highly recommended for supplementing the human body with better nutrition density, dietary fiber, minerals, vitamins, and bioactive compounds. However, the production and quality of fresh vegetables are influenced directly or indirectly by exposure to high temperatures or heat stress (HS). A decline in quality traits and harvestable yield are the most common effects of HS among vegetable crops. Heat-induced morphological damage, such as poor vegetative growth, leaf tip burning, and rib discoloration in leafy vegetables and sunburn, decreased fruit size, fruit/pod abortion, and unfilled fruit/pods in beans, are common, often rendering vegetable cultivation unprofitable. Further studies to trace down the possible physiological and biochemical effects associated with crop failure reveal that the key factors include membrane damage, photosynthetic inhibition, oxidative stress, and damage to reproductive tissues, which may be the key factors governing heat-induced crop failure. The reproductive stage of plants has extensively been studied for HS-induced abnormalities. Plant reproduction is more sensitive to HS than the vegetative stages, and affects various reproductive processes like pollen germination, pollen load, pollen tube growth, stigma receptivity, ovule fertility and, seed filling, resulting in poorer yields. Hence, sound and robust adaptation and mitigation strategies are needed to overcome the adverse impacts of HS at the morphological, physiological, and biochemical levels to ensure the productivity and quality of vegetable crops. Physiological traits such as the stay-green trait, canopy temperature depression, cell membrane thermostability, chlorophyll fluorescence, relative water content, increased reproductive fertility, fruit numbers, and fruit size are important for developing better yielding heat-tolerant varieties/cultivars. Moreover, various molecular approaches such as omics, molecular breeding, and transgenics, have been proved to be useful in enhancing/incorporating tolerance and can be potential tools for developing heat-tolerant varieties/cultivars. Further, these approaches will provide insights into the physiological and molecular mechanisms that govern thermotolerance and pave the way for engineering “designer” vegetable crops for better health and nutritional security. Besides these approaches, agronomic methods are also important for adaptation, escape and mitigation of HS protect and improve yields.

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Comparative effects of glycinebetaine on the thermotolerance in codA- and BADH-transgenic tomato plants under high temperature stress

Cited by 22 publications

References 59 publications

Glycinebetaine mitigates drought stress-induced oxidative damage in pears

Glycinebetaine mitigates drought stress-induced oxidative damage in pears

Role of Glycine Betaine in the Thermotolerance of Plants

Physiological and Molecular Approaches for Developing Thermotolerance in Vegetable Crops: A Growth, Yield and Sustenance Perspective

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