Comparative effects of injecting 5,6-dihydroxytryptamine in the dorsal or medial raphe nuclei on rat puberty

Monroy, Juana; Ayala, Marı́a Elena; Chavira, Roberto; Damián-Matsumura, Pablo; Domı́nguez, Roberto

doi:10.1016/s0361-9230(03)00044-3

Cited by 9 publications

(7 citation statements)

References 25 publications

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“…Previously, we showed that in 30-day-old female rats, the microinjection of 5,6-dihydroxytryamine, a selective neurotoxin of the serotonergic fibres, into the MRN does not modify the secretion of LH (Monroy et al, 2003). Therefore, the role of serotonergic innervation from the MRN to the hypothalamus in gonadotrophin secretion and control of steroidogenesis in the ovary remains to be established.…”

Section: Highlightsmentioning

confidence: 95%

The differential sensitivity of the hypothalamic–hypophysial–ovarian axis to 5‐hydroxytryptophan alters the secretion of estradiol

Monroy,

Cortés,

Domínguez

et al. 2023

Experimental Physiology

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Serotonin [5‐hydroxytryptamine (5‐HT)] modulates ovarian function. The precursor of 5‐HT, 5‐hydroxytryptophan (5‐HTP), has been used to treat depression. However, the effects of 5‐HTP on ovarian and reproductive physiology remain unknown. In this research, we analysed the impact of 5‐HTP on the monoaminergic system and its interactions with the reproductive axis and ovarian estradiol secretion when administered by distinct routes. Female rats 30 days of age were injected with 5‐HTP i.p. (100 mg/kg), into the ovarian bursa (1.5 µg/40 µL) or into the median raphe nucleus (20 µg/2.5 µL) and were killed 60 or 120 min after injection. As controls, we used rats of the same age injected with vehicle (0.9% NaCl). Monoamine, gonadotrophin and steroid ovarian hormone concentrations were measured. The injection of 5‐HTP either i.p. or directly into the ovarian bursa increased the concentrations of 5‐HT and the metabolite 5‐hydroxyindole‐3‐acetic acid in the ovary. For both routes of administration, the serum concentration of estradiol increased. After i.p. injection of 5‐HTP, the concentrations of luteinizing hormone were decreased and follicle‐stimulating hormone increased after 120 min. Micro‐injection of 5‐HTP into the median raphe nucleus increased the concentrations of 5‐HT in the anterior hypothalamus and dopamine in the medial hypothalamus after 120 min. Our results suggest that the administration of 5‐HTP either i.p. or directly into the ovarian bursa enhances ovarian estradiol secretion.

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Section: Highlightsmentioning

confidence: 95%

The differential sensitivity of the hypothalamic–hypophysial–ovarian axis to 5‐hydroxytryptophan alters the secretion of estradiol

Monroy,

Cortés,

Domínguez

et al. 2023

Experimental Physiology

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“…10 μg of 5,6-DHT (Sigma Chemical Co., St Louis, Mo, USA) dissolved in 2.5 μl of 0.05 % ascorbic acid (pH 3.5) was injected at an infusion rate of 1 μl/min. 5,6-DHT is a serotonergic-specific neurotoxin [ 6 ]. The 5,6-DHT dose was selected based on previous experiments, because 10 μg generated a selective depletion of serotonin concentration in the hypothalamus (50 %) but does not impact catecholamine concentration [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

“…The amounts of serotonin (5-HT), noradrenaline (NA), and dopamine (DA), were measured using the methodology described by Monroy et al . [ 6 ]. Briefly, tissue samples were weighed and homogenized in 300 μl of 0.1 N perchloric acid and subsequently centrifuged at 12500 rpm for 30 min at −4 °C.…”

Section: Methodsmentioning

confidence: 99%

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Dorsal and medial raphe nuclei participate differentially in reproductive functions of the male rat

Ayala¹,

Velázquez²,

Mendoza³

et al. 2015

Reprod Biol Endocrinol

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BackgroundInnervation of the hypothalamus and median eminence arise from the dorsal and medial raphe nuclei (DRN and MRN, respectively). The hypothalamus regulates the secretion of gonadotropins, which in turn regulate the reproductive function of males and females. However, it is not known the role of raphe nuclei in male reproductive function. Our goal was to investigate the role of the DRN and MRN in the regulation of the testicular function and secretion of gonadotropins in prepubertal rats.MethodsDihydroxytryptamine (5,6-DHT) in ascorbic acid was used to chemically lesion the DRN or MRN. Rats were treated at 30 days-of-age and sacrificed at 45 or 65 days-of-age. Sham-treated controls were injected with ascorbic acid only. Negative controls were untreated rats. The damage induced by the 5,6-DHT was monitored in coronal serial sections of DRN and MRN; only the animals in which lesion of the DRN or MRN was detected were included in this study. As output parameters, we measured the concentrations of noradrenaline (NA), dopamine (DA) and serotonin (5-HT) in the anterior (AH) and medial (MH) hypothalamus by high performance liquid chromatography (HPLC); whereas, circulating concentrations of gonadotropins and sexual steroids were measured by radioimmunoassay. Seminiferous epithelium and sperm quality were also evaluated.ResultsLesion of DRN or MRN does not induced changes in concentrations of LH, progesterone, and testosterone. Compared with the control group, the sham or lesion of the DRN or MRN did not modify noradrenaline or dopamine concentrations in the AH and MH at 45 or 65 days of age. Meanwhile, serotonin concentrations decreased significantly in lesioned rats. Lesion of DRN induced significantly lower concentrations of FSH regardless of age; similar lesion in the MRN had no impact on FSH levels. Sperm concentration and motility were significantly decreased in the same animals. The lesion of the MRN does not induced changes in the seminiferous epithelium or gonadotropin levels. Our results suggest that raphe nuclei regulate differentially the male reproductive functions.ConclusionsThe DRN but not the MRN regulates the secretion of gonadotropins and testicular function.Electronic supplementary materialThe online version of this article (doi:10.1186/s12958-015-0130-0) contains supplementary material, which is available to authorized users.

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“…One possible function of this temporary upregulation of the serotonergic system might be pruning, which means that synapses and perhaps receptors are dselectedT from a surplus to retain in adulthood, depending on the experiences and environment of the adolescent rats (Andersen and Navalta, 2004). An additional function might be the serotonin-mediated onset of puberty in male (Shishkina and Dygalo, 2000) and female (Monroy et al, 2003) rats.…”

Section: Introductionmentioning

confidence: 99%

Effects of chronic treatment with fluvoxamine and paroxetine during adolescence on serotonin-related behavior in adult male rats

Jong

Snaphaan

Pattij

et al. 2006

European Neuropsychopharmacology

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Selective Serotonin Reuptake Inhibitors (SSRIs) are designed to treat adults, but are increasingly prescribed for adolescents. SSRIs might cause permanent changes in serotonin-related behavior in adolescents, since their serotonergic system is still developing.Male Wistar rats were treated with paroxetine (15 mg/kg p.o.) or fluvoxamine (30 mg/kg p.o.) throughout adolescence. After a washout period their behavior in the elevated plusmaze, prepulse inhibition test, Forced swimming test and elevated T-maze were studied. In addition, the effects of the 5-HT 1A receptor agonist 8-OH-DPAT on sexual behavior and lower lip retraction were measured. Paroxetine mildly inhibited weight gain during treatment. Both SSRIs caused a reduction in ejaculation frequency and in time spent on the open arm of the elevated plus-maze in adult rats. avoidance latency in the elevated T-maze compared to paroxetine. No differences between the groups were found in the other tests. Apparently, chronic treatment with SSRIs during adolescence may cause mild changes in adult behavior.

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Comparative effects of injecting 5,6-dihydroxytryptamine in the dorsal or medial raphe nuclei on rat puberty

Cited by 9 publications

References 25 publications

The differential sensitivity of the hypothalamic–hypophysial–ovarian axis to 5‐hydroxytryptophan alters the secretion of estradiol

The differential sensitivity of the hypothalamic–hypophysial–ovarian axis to 5‐hydroxytryptophan alters the secretion of estradiol

Dorsal and medial raphe nuclei participate differentially in reproductive functions of the male rat

Effects of chronic treatment with fluvoxamine and paroxetine during adolescence on serotonin-related behavior in adult male rats

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