Comparative Effects of Platelet-Rich Plasma, Platelet Lysate, and Fetal Calf Serum on Mesenchymal Stem Cells

Лыков, А. П.; Bondarenko, N. A.; Суровцева, М. А.; Kim, I. I.; Повещенко, О. В.; Pokushalov, E. A.; Vi, Konenkov

doi:10.1007/s10517-017-3897-5

Cited by 14 publications

(8 citation statements)

References 12 publications

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“…Some studies have suggested that FBS can be replaced with PRP to induce stem cell proliferation [14], with 15% PRP having the highest proliferation rate [14,64]. The results of our study do not sustain this idea.…”

Section:  Discussioncontrasting

confidence: 93%

Injectable platelet-rich fibrin influences the behavior of gingival mesenchymal stem cells

Iozon

Caracostea

Páll³

et al. 2020

Rom J Morphol Embryol

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In this study, we examined the effects of injectable platelet-rich fibrin (iPRF) on proliferation and osteodifferentiation in mesenchymal stem cells (MSCs) isolated from human gingiva. Gingival MSCs (gMSCs) were grown in experimental culture media with different concentrations of iPRF [5%, 10%, and replacement of fetal calf serum (FCS) in the standard media with 10% iPRF-10% iPRF-FCS]. Immunophenotyping of gMSCs was performed after seven days by flow cytometry, and their proliferation was examined after three and seven days using the Cell Counting Kit-8 method. After 14 days in culture, spontaneous osteogenic differentiation of gMSCs was evaluated via real-time polymerase chain reaction. All gMSCs were positive for cluster of differentiation (CD) 105, CD73, CD90, and CD44, and negative for CD34/45, CD14, CD79a, and human leukocyte antigen, DR isotype (HLA-DR). Reduced expression of some surface antigens was observed in the gMSCs grown in 10% iPRF-FCS medium compared to the other groups. After three days, gMSCs grown in 10% iPRF had proliferated significantly less than the other groups. After seven days, proliferation was significantly higher in the 5% iPRF cells compared to the control, while proliferation in the 10% iPRF and 10% iPRF-FCS groups was significantly lower. No spontaneous osteogenic differentiation was observed in the presence of iPRF, as observed by low runt-related transcription factor 2 (RUNX2) expression. Some expression of secreted protein acidic and cysteine rich (SPARC) and collagen 1 alpha (COL1A) was observed for all the gMSCs regardless of the culture medium composition. gMSCs grown in 10% iPRF had significantly lower SPARC expression. In conclusion, 5% iPRF stimulated gMSC proliferation, and an excessively high concentration of iPRF can impair osteogenic induction.

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Section:  Discussioncontrasting

confidence: 93%

Injectable platelet-rich fibrin influences the behavior of gingival mesenchymal stem cells

Iozon

Caracostea

Páll³

et al. 2020

Rom J Morphol Embryol

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“…Studies have revealed that there are many growth factors contained in hPL that promote MSC proliferation and differentiation, including basic fibroblast growth factor (bFGF), EGF, insulin-like growth factor 1 (IGF-1), PDGF, VEGF and TGF-β (7,9,23). As has been reported previous studies, hPL is often used at a concentration of 10% (9,24,25). Consequently, this concentration was selected to evaluate MSC characteristics.…”

Section: Discussionmentioning

confidence: 83%

Human platelet lysate as an alternative to fetal bovine serum for culture and endothelial differentiation of human amniotic fluid mesenchymal stem cells

et al. 2019

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Human amniotic fluid (hAF) mesenchymal stem cells (MSCs) are commonly cultured in medium containing FBS. However, there are concerns about using animal serum in therapeutic applications due to the potential for immunogenic reactions and the risk of transmission of pathogens. For safety reasons, human platelet lysate (hPL) has been suggested as a replacement for FBS because it appears to be a natural source of growth factors. In this present study, it was investigated whether FBS could be substituted with hPL in hAF-MSCs culture without affecting their properties. Pooled hPL was generated by the freeze-thaw method. The concentration of hPL was selected after evaluation by MTT assay. The hAF-MSCs were cultured in FBS- or hPL-supplemented conditions and shared a fibroblast-like morphology. Cell proliferation assays showed that the growth characteristic of hAF-MSCs cultured in 10% hPL-supplemented media was similar to those cultured in 10% FBS-supplemented media. The expression of MSC markers did not differ between the cells cultured in the different conditions. The endothelial differentiation potential was also investigated. Reverse transcription-quantitative (RT-q)PCR revealed that induced cells supplemented with hPL showed an increase level of endothelial specific gene expression compared to the FBS-supplemented cells. Immunofluorescence analysis showed specific protein localization in both induced cell groups. Additionally, induced cells supplemented with hPL had the potential to form networks on Matrigel. This present study indicated that hPL could be used to culture and enhance the endothelial differentiation potential of hAF-MSCs.

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“…Furthermore, cells grown in xenogeneic-free hPL are therapeutically useful (Sergeeva et al, 2016;Riis et al, 2016;Thieme et al, 2017). They could be used for bone regeneration, osteoarthritis, the therapy of stroke, wound healing, and other applications (Tan et al, 2016;Sergeeva et al, 2016;Altaie et al, 2016;Martinelli et al, 2016;Riis et al, 2016;Lykov et al, 2017;Thieme et al, 2017;Leijs et al, 2017). Indeed, bone marrow stromal cells cultured in hPL are currently being tested in a phase I study involving patients with acute ischemic stroke (Shichinohe et al, 2017).…”

Section: Cell Lines and Adaptation Processmentioning

confidence: 99%

Human platelet lysate as validated replacement for animal serum to assess chemosensitivity

Pons

Nagel

Zeyn

et al. 2018

ALTEX

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otherwise not engaged signaling pathways (Moore et al., 2001). Moreover, the use of FCS is incompatible with donations of cells to humans for therapeutic purposes (Barsotti et al., 2013; Doucet et al., 2005). Due to these concerns, alternative strategies to the use of FCS have been considered.While fully recombinant serum replacements are still a cost issue, human platelet lysate (hPL) could be a valid and ethically justifiable option. Human keratinocyte cells, renal epithelial cells, and various leukemic and solid tumor cell lines can be propagated in hPL (Fazzina et al., 2016;Rauch et al., 2011; Baik et al., 2014; Bernardi et al., 2013). This also holds true for primary human and rodent cells (adipocytes, amniotic fluid stem cells, bone marrow stromal cells, chondrocytes, corneal cells, endothelial cells, keratinocytes, mesenchymal stem cells, monocytes, osteoblasts) (Barsotti et al., 2013; Doucet et al., 2005;Glovinski et al., 2017). These cell types maintain functionality and signaling in various assays, and some reports even show a better cell proliferation in hPL than in FCS (Bernardi et al., 2013;Glovinski et al.,

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Comparative Effects of Platelet-Rich Plasma, Platelet Lysate, and Fetal Calf Serum on Mesenchymal Stem Cells

Cited by 14 publications

References 12 publications

Injectable platelet-rich fibrin influences the behavior of gingival mesenchymal stem cells

Injectable platelet-rich fibrin influences the behavior of gingival mesenchymal stem cells

Human platelet lysate as an alternative to fetal bovine serum for culture and endothelial differentiation of human amniotic fluid mesenchymal stem cells

Human platelet lysate as validated replacement for animal serum to assess chemosensitivity

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