Comparative effects of schisandrin A, B, and C on Propionibacterium acnes-induced, NLRP3 inflammasome activation-mediated IL-1β secretion and pyroptosis

Guo, Miaomiao; An, Faliang; Yu, Haiyuan; Xing, Wei; Hong, Minhua; Lü, Yanhua

doi:10.1016/j.biopha.2017.09.097

Cited by 47 publications

(45 citation statements)

References 42 publications

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“…The suppression of inflammasome components and pyroptosis may explain the mechanism of the neuroprotective effects of Sch B. Our study results are similar to those of Guo et al [13]. In their study, they found that Sch B effectively suppresses inflammasome activation and pyroptosis and therefore inhibits inflammation [13].…”

Section: Discussionsupporting

confidence: 91%

Albendazole-Schisandrin B Co-Therapy on Angiostrongylus cantonensis-Induced Meningoencephalitis in Mice

et al. 2020

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Currently, Angiostrongylus cantonensis infections are predominantly treated with albendazole. However, the use of albendazole can provoke certain neurological symptoms as a result of the immune response triggered by the dead worms. Therefore, treatment usually involves co-administration of corticosteroids to limit the inflammatory reaction. Corticosteroids play a useful role in suppressing inflammation in the brain; however, long-term usage or high dosage may make it problematic.Schisandrin B, an active ingredient from Schisandra chinensis, has been shown to have anti-inflammatory effects on the brain. This study aimed to investigate the effects and potential of schisandrin B in combination with albendazole to treat Angiostrongylus-induced meningoencephalitis. Here, we show that albendazole-schisandrin B co-treatment suppressed neuroinflammation in Angiostrongylus-infected mice and increased the survival of the mice. Accordingly, albendazole-schisandrin B co-treatment significantly inhibited inflammasome activation, pyroptosis, and apoptosis. The sensorimotor functions of the mice were also repaired after albendazole-schisandrin B treatment. Immune response was shown to shift from Th2 to Th1, which reduces inflammation and enhances immunity against A. cantonensis. Collectively, our study showed that albendazole-schisandrin B co-therapy may be used as an encouraging treatment for Angiostrongylus-induced meningoencephalitis.

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Section: Discussionsupporting

confidence: 91%

Albendazole-Schisandrin B Co-Therapy on Angiostrongylus cantonensis-Induced Meningoencephalitis in Mice

et al. 2020

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“…4B). Correspondingly, the serum levels of caspase-1 activity and mature IL-1β 18 were significantly increased in the IRI group compared to the sham control ( Fig. 1b).…”

Section: Resultsmentioning

confidence: 77%

Blocking GSDMD processing in innate immune cells but not in hepatocytes protects hepatic ischemia–reperfusion injury

Zhao

et al. 2020

Cell Death Dis

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Pyroptosis, a proinflammatory form of programmed cell death, plays important roles in the pathogenesis of many diseases. Inflammasome activation, which has been shown in hepatic ischemia-reperfusion injury (IRI), is demonstrated to be closely associated with pyroptosis, indicating that pyroptosis may occur and perform functions in hepatic IRI. However, there is no direct evidence showing the function of pyroptosis in hepatic IRI. In this study, by detecting the pyroptosis markers, we showed that pyroptosis may be induced during hepatic IRI. Furthermore, by adopting caspase-1 inhibitors, we showed that inhibition of pyroptosis could significantly ameliorate liver injury and suppress inflammatory response during hepatic IRI. Interestingly, caspase-1 inhibitors have no protective effects on in vitro hepatocytes under hypoxic reoxygenation condition. To investigate pyroptosis induced in which specific cell types may affect hepatic IRI, we generated hepatocyte-specific Gsdmd-knockout (Hep-Gsdmd −/−) and myeloidspecific Gsdmd-knockout (LysmCre + Gsdmd f/f) mice. Functional experiments showed that compared to control mice (Gsdmd f/f), there were alleviated liver injury and inflammation in LysmCre + Gsdmd f/f mice, but not in AlbCre + Gsdmd f/f mice. In parallel in vitro studies, cytokine expression and production decreased in bone-marrow-derived macrophages and Kupffer cells from LysmCre + Gsdmd f/f mice compared to their controls. Our findings demonstrated that pyroptosis in innate immune cells aggravates hepatic IRI and implied that hepatic IRI could be protected by blocking pyroptosis, which may become a potential therapeutic target in the clinic.

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“…After incubation under anaerobic conditions for 24 h, the level of microbial growth was tested using a microplate reader at 600 nm. The MIC was defined as the lowest dilution of walrobsins at which growth was inhibited completely 20. , 21.…”

Section: Methodsmentioning

confidence: 99%

“…The formazan crystals were solubilized in 150 μL dimethyl sulfoxide after 4 h incubation. Absorbance was measured by a spectrophotometer at 570 nm excitation and 630 nm emission 20. , 21.…”

Section: Methodsmentioning

confidence: 99%

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Bioactive A-ring rearranged limonoids from the root barks of Walsura robusta

Wang

Yang

et al. 2019

Acta Pharmaceutica Sinica B

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Screening active natural products, rapid identification, and accurate isolation are of great important for modern natural lead compounds discovery 1 . We hereby reported the isolation of seven new neotecleanin-type limonoids ( 1 — 7 ), seven new limonoids with 5-oxatricyclo[5.4.0.1 1. , 4. ]hendecane ring system ( 8 — 14 ), and two new precursors ( 15 — 16 ) together with four known limonoids ( 17 — 20 ) from the root barks of Walsura robusta . Their structures, including their absolute configurations, were elucidated based on analyses of HR-ESI-MS, 1D/2D NMR, ECD spectrum calculations and single-crystal X-ray diffraction techniques. Compounds 2 , 8 , 9 , 11 , 13 , 14 , 18 showed significant anti-inflammatory activities in LPS-induced RAW 264.7 cell line, BV2 microglial cells, and Propionibacterium acnes -stimulated THP-1 human monocytic cells. Walrobsin M ( 11 ) exhibited anti-inflammatory activity with IC 50 value of 7.96±0.36 μmol/L, and down-regulated phosphorylation levels of ERK and p38 in a dose-dependent manner.

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Comparative effects of schisandrin A, B, and C on Propionibacterium acnes-induced, NLRP3 inflammasome activation-mediated IL-1β secretion and pyroptosis

Cited by 47 publications

References 42 publications

Albendazole-Schisandrin B Co-Therapy on Angiostrongylus cantonensis-Induced Meningoencephalitis in Mice

Albendazole-Schisandrin B Co-Therapy on Angiostrongylus cantonensis-Induced Meningoencephalitis in Mice

Blocking GSDMD processing in innate immune cells but not in hepatocytes protects hepatic ischemia–reperfusion injury

Bioactive A-ring rearranged limonoids from the root barks of Walsura robusta

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