Comparative effects of well-balanced diets enriched in α-linolenic or linoleic acids on LC-PUFA metabolism in rat tissues

Blanchard, H.; Pédrono, Frédérique; Boulier-Monthéan, Nathalie; Catheline, Daniel; Rioux, Vincent; Legrand, Philippe

doi:10.1016/j.plefa.2013.03.006

Cited by 47 publications

(45 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Interestingly, it has been proposed that such high ratios might represent a significant risk factor in some life-threatening diseases (Simopoulos 2008), including the loss of bone mass density (Jarvinen et al 2012). Even more, recent animal studies have shown that diets with both omega-6 and omega-3 fatty acids may alter the tissue lipidic composition, with a potentially healthier production of inflammatory cytokines when such ratio approaches the value of one (Blanchard et al 2013;Duan et al 2013). Nevertheless, the significance of these findings in the development of some diseases remains to be further studied and validated in well-qualified in vitro/ in vivo experimental models, as well as in epidemiological and intervention studies.…”

Section: Discussionmentioning

confidence: 99%

Effects of arachidonic acid on the concentration of hydroxyeicosatetraenoic acids in culture media of mesenchymal stromal cells differentiating into adipocytes or osteoblasts

et al. 2013

View full text Add to dashboard Cite

Metabolites derived from the polyunsaturated fatty acids (PUFA) may modulate the mesenchymal stromal cell (MSC) differentiation. Such cells can differentiate into different cellular types, including adipocytes and osteoblasts. Aging favors the bone marrow MSC differentiation toward the former, causing a loss of bone density associated with pathologies like osteoporosis. The omega-6 arachidonic acid (AA) favors MSC adipogenesis to a greater extent than omega-3 eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). In this work, we study the joint action of both PUFA. Thus, not induced and induced to adipocyte or osteoblast MSC were treated with 20 lM of each PUFA (either AA, AA ? DHA or AA ? EPA). The expression of osteogenic and adipogenic molecular markers, the alox15b lipoxygenase gene expression and the 5-, 8-, 11-, 12-and 15-hydroxyeicosatetraenoic acids (HETE) derived from the AA metabolism in the culture media were determined. The results show that the adipogenesis induction of AA is not suppressed by the joint presence of EPA and DHA. In fact, both increased the adipogenic effect of AA on MSC differentiated into osteoblasts. The different HETE concentrations increased in cultures supplemented with AA, albeit such concentrations were lower in the cultures induced to differentiate, mainly at day 21 after the induction. Furthermore, the reduction in the HETE concentration was correlated with a higher expression of the alox15b gene. These results highlight the PUFA metabolism differences between uninduced and induced MSC to differentiate into adipocytes and osteoblasts, besides the relevant role of the lipoxygenase gene expression in adipogenesis induction.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of arachidonic acid on the concentration of hydroxyeicosatetraenoic acids in culture media of mesenchymal stromal cells differentiating into adipocytes or osteoblasts

et al. 2013

View full text Add to dashboard Cite

show abstract

“…The liver was fractioned in a phosphate buffer containing 0.25 M sucrose to recover the post-mitochondrial supernatant as previously described [18]. Enzymatic activities were assayed in duplicates with different substrates: [1-14 C]20:3n-6 for Δ5-desaturase and [1-14 C]18:1n-9, [1-14 C]18:2n-6, and [1-14 C]18:3n-3 for Δ6-desaturase (60 µmol/L, 10 mCi/mmol).…”

Section: δ5-and δ6-desaturase Activity Assaysmentioning

confidence: 99%

Impact of a Standard Rodent Chow Diet on Tissue n‐6 Fatty Acids, Δ9‐Desaturation Index, and Plasmalogen Mass in Rats Fed for One Year

Pédrono

Boulier-Monthéan

Catheline

et al. 2015

Lipids

Self Cite

View full text Add to dashboard Cite

Although many studies focus on senescence mechanisms, few habitually consider age as a biological parameter. Considering the effect of interactions between food and age on metabolism, here we depict the lipid framework of 12 tissues isolated from Sprague-Dawley rats fed standard rodent chow over 1 year, an age below which animals are commonly studied. The aim is to define relevant markers of lipid metabolism influenced by age in performing a fatty acid (FA) and dimethylacetal profile from total lipids. First, our results confirm impregnation of adipose and muscular tissues with medium-chain FA derived from maternal milk during early infancy. Secondly, when animals were switched to standard croquettes, tissues were remarkably enriched in n-6 FA and especially 18:2n-6. This impregnation over time was coupled with a decrease of the desaturation index and correlated with lower activities of hepatic Δ5- and Δ6-desaturases. In parallel, we emphasize the singular status of testis, where 22:5n-6, 24:4n-6, and 24:5n-6 were exceptionally accumulated with growth. Thirdly, 18:1n-7, usually found as a discrete FA, greatly accrued over the course of time, mostly in liver and coupled with Δ9-desaturase expression. Fourthly, skeletal muscle was characterized by a surprising enrichment of 22:6n-3 in adults, which tended to decline in older rats. Finally, plasmalogen-derived dimethylacetals were specifically abundant in brain, erythrocytes, lung, and heart. Most notably, a shift in the fatty aldehyde moiety was observed, especially in brain and erythrocytes, implying that red blood cell analysis could be a good indicator of brain plasmalogens.

show abstract

“…Toutefois, les études s'accordent à dire que l'ALA serait un excellent substrat pour la b-oxydation et qu'une majeure partie serait b-oxydée (15 à 33 %) (Burdge et Wootton, 2002 ;Burdge, 2004 ;Arterburn et al, 2006). Seule une faible proportion serait convertie en EPA (8 à 21 %) et en DHA (0,05 à 9 %) (Burdge et Wootton, 2002 ;Burdge, 2004 ;Harnack et al, 2009 ;Blanchard et al, 2013).…”

Section: Introductionunclassified

Matrice lipidique et biodisponibilité de l’acide alpha-linolénique

Couëdelo

Termon

Vaysse

2017

OCL

View full text Add to dashboard Cite

Résumé -Les acides gras poly-insaturés (AGPI) de la série oméga-3 (v3) ont un rôle important dans la prévention de certaines pathologies. En plus d'être nécessaires dans des conditions physiologiques particulières (développement pré-et post-natal, croissance) (Riediger et al., 2009. J Am Diet Assoc 109 : 668-679), ils sont associés à des effets santé en termes de prévention, notamment au niveau de pathologies cardiovasculaires, inflammatoires, certains cancers et certaines maladies neuro-dégénératives (De Lorgeril et al., 1994. Lancet 343 : 1454-1459Simopoulos, 2008. Exp Biol Med (Maywood, NJ) 233 : 674-688). Cependant, les dernières études épidémiologiques montrent que les apports en AGPI v3, et notamment en acide alpha-linolénique (ALA), précurseur métabolique des AGPI à longue chaîne v3, sont deux fois inférieurs aux recommandations de l'Agence national de sécurité sanitaire, de l'alimentation, de l'environnement et du travail (ANSES, 2011). Outre la nécessité d'augmenter l'apport en ALA, il est désormais nécessaire de prendre en considération les facteurs qui améliorent sa biodisponibilité. Dans ce contexte, nous avons testé plusieurs paramètres susceptibles de moduler le devenir métabolique de l'ALA. Nos recherches ont mis en évidence que plusieurs paramètres physiques et chimiques, tels que l'émulsification d'une huile linolénique avec de la lécithine de soja, la position de l'ALA sur le squelette glycérique du triglycéride alimentaire mais aussi la composition de la matrice permettraient de moduler la biodisponibilité et le devenir métabolique de l'ALA dans l'organisme.Mots clés : alpha-linolénique / biodisponibilité / émulsion / structure glycéridique / matrice lipidique Abstract -Lipid matrix and bioavailability of alpha-linolenic acid. Polyunsaturated fatty acids (PUFA) of omega-3 series (v3) have an important role in preventing certain diseases. Besides being necessary in specific physiological conditions (pre-and post-natal development, growth) (Riedigeret al., 2009. J Am Diet Assoc 109: 668-679.), they are associated with health effects in terms of prevention, especially for cardiovascular disease, inflammation, some cancers and neurodegenerative diseases (De Lorgeril et al., 1994. Lancet 343: 1454-1459Simopoulos, 2008. Exp Biol Med (Maywood, NJ) 233: 674-688). However, recent epidemiological studies show that the PUFA v3 intake, including alpha-linolenic acid (ALA), the metabolic precursor of long chain PUFA v3, are three times lower than the French recommendations (ANSES, 2011). Besides the need to increase the ALA intake, it is now necessary to take into account the factors that improve its bioavailability. In this context, we tested several parameters that modulate the metabolic fate of ALA. Our research has demonstrated that several physical and chemical parameters, such as the emulsification of linolenic oil with soybean lecithin, the position of ALA on the glycerol backbone of the triglyceride but also the composition of the lipid matrix would modulate the bioavailability and the metabolic f...

show abstract

Comparative effects of well-balanced diets enriched in α-linolenic or linoleic acids on LC-PUFA metabolism in rat tissues

Cited by 47 publications

References 34 publications

Effects of arachidonic acid on the concentration of hydroxyeicosatetraenoic acids in culture media of mesenchymal stromal cells differentiating into adipocytes or osteoblasts

Effects of arachidonic acid on the concentration of hydroxyeicosatetraenoic acids in culture media of mesenchymal stromal cells differentiating into adipocytes or osteoblasts

Impact of a Standard Rodent Chow Diet on Tissue n‐6 Fatty Acids, Δ9‐Desaturation Index, and Plasmalogen Mass in Rats Fed for One Year

Matrice lipidique et biodisponibilité de l’acide alpha-linolénique

Contact Info

Product

Resources

About