Comparative efficacy of dermal fibroblast-mediated and direct adenoviral bone morphogenetic protein-2 gene therapy for bone regeneration in an equine rib model

Innovative Collagen Nano‐Hydroxyapatite Scaffolds Offer a Highly Efficient Non‐Viral Gene Delivery Platform for Stem Cell‐Mediated Bone Formation

“…[2,3] However, using viral vectorbased technology possesses numerous disadvantages not least the known safety concerns associated with insertational mutagenesis following viral integration [4] and the adverse immune responses observed in patients. [5] Additional problems include the time consuming and labour intensive process involved in Innovative Collagen Nano-Hydroxyapatite Scaffolds Offer a Highly Efficient Non-Viral Gene Delivery Platform for Stem Cell-Mediated Bone Formation the production of such viruses, thereby limiting their potential for tissue engineering.…”

Healing of massive segmental femoral bone defects in minipigs by allogenic ASCs engineered with FLPo/Frt-based baculovirus vectors

“…In one study, BMSCs were engineered with the adenovirus expressing BMP7 (AdBMP7), seeded into scaffolds and implanted into the critical-size (25 mm in length) segmental femoral defects in goats [32]. Alternatively, equine dermal fibroblasts engineered with the adenovirus expressing BMP2 (AdBMP2) were directly injected into the equine rib drill defects [33] or into horses with metacarpal/metatarsal osteotomies [34].…”

Cell-mediated and direct gene therapy for bone regeneration

“…Ad-BMP2 gene transfer induced greater bone regeneration in mice calvarial bone defects than Ad-Runx2 [63] DFb Ad-BMP2 Improved bone regeneration in equine metatarsal defects [64] DFb Ad-BMP2 Improved bone regeneration in equine rib drill defects [65] DFb Retro-BMP2 Ectopic bone formation in mice [66] DFb Ad-BMP7 Improved bone regeneration in rat cranial defects [67] DFb Ad-LMP3 Spine fusion and improved mandible defect regeneration in mice [68] DFb and BMD-MSC Ad-BMP2 Comparison of ectopic bone formation between DFb and BMD MSC in mice [69] Blood Buffy coat cells Ad-LMP1 Ectopic bone formation in rats [18] Buffy coat cells Ad-LMP1 Spine fusion in rabbits [70] Umbilical cord blood-derived MSC N/A Cells administered with beta-tricalcium phosphate improved the canine radial defect regeneration [71] Peripheral blood mononuclear cells N/A Cells administered with osteoinductive supplements improved the rat calvarial defect regeneration [72] Ad shown to undergo multilineage differentiation into bone, and cartilage, neuron, endothelial and hematopoietic tissues [79]. Unlike BMD-MSC, muscle-derived inducible osteoprogenitors do not express osteogenic markers until exposed to BMP2 [56]; therefore, the MDSC should be used in combination with osteoinductive factors to achieve full osteogenic potential.…”