Comparative efficacy of four anaesthetic agents in the yellow seahorse, Hippocampus kuda (Bleeker, 1852)

Pawar, H.B.; Sanaye, S.V.; Sreepada, R.A.; Harish, V.; Suryavanshi, U.; Tanu, .; Ansari, Z.A.

doi:10.1016/j.aquaculture.2010.12.007

Cited by 60 publications

(72 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The induction time in this study were found to be lower in juveniles' fish when compared to adult fish. This agrees with the findings of Hseu et al [35], in gold lined sea bream, (Sparus sarba) and that of Pawar et al [36], in yellow horse (Hippocampus kuda). The shorter induction time in juvenile fish when compared to adult may be due to small body size and reduced surface area of the gill in juvenile fish [37].…”

Section: Discussionsupporting

confidence: 83%

The Efficacy of Clove Seed Extracts as an Anaesthetic Agent and Its Effect on Haematological Parameters of African Catfish (Clarias Gariepinus)

Akinrotimi¹

2015

Int J Aquac Fish Sci

View full text Add to dashboard Cite

Background and Aim: The intensive nature of aquaculture has subjected fish to a number of stressors in the culture medium, anesthetics are widely used to minimize the issue of stress during farming operations and activities. Clove oil is a well known, established and acceptable anesthetics commonly used in aquaculture, this anesthetics is not readily available in developing countries, thus leaving the fish farmers with option of using the locally available plant extracts as anesthetic agent in fish culture. This study therefore assessed the efficacy of aqueous extracts of clove seed and its effect on hematological parameters of the fish.Methods: A total of 300 Clarias gariepinus comprises of 150 juveniles and 150 adult fish were exposed in triplicates to aqueous extracts of clove seed at concentrations of 5.0, 10.0, 15.0, 20.0 and 25.0mg/l at the rate of 10 fish per tank for both juveniles and adult fish. Induction time (Time taken for the fish to be completely anaesthetized) and recovery time (Time taken for the fish to resume normal swimming) was monitored using stop watch. Also, the effect of the extracts on water quality, survival and hematological parameters of the fish were evaluated using standard methods. Results:The result obtained revealed that the induction time indicated a size related response with the adult fish significantly (P<0.05) higher than the juveniles at all concentration of exposure. However, reverse was the case in recovery time, as the recover times in juveniles were higher than the adult fish. The survival was 100% in both sizes. Significant alterations were recorded in the haematological variables of the fish, which was more pronounced at higher concentrations of the extracts.

show abstract

Section: Discussionsupporting

confidence: 83%

The Efficacy of Clove Seed Extracts as an Anaesthetic Agent and Its Effect on Haematological Parameters of African Catfish (Clarias Gariepinus)

Akinrotimi¹

2015

Int J Aquac Fish Sci

View full text Add to dashboard Cite

show abstract

“…At 500 μL LG 1 , the time to reach anaesthesia stage (I 3 ) of induction (159±13 sec) and recovery (R 3 ) time (133±16 sec) was significantly different (p<0.05) from the other dosages 400 μL LG 1 ( 193±14 sec), 300 μL LG 1 ( 216±78 sec) and 600 μL LG 1 ( 190±14 sec) (Table 1). These results are consistent with previous studies in teleost fishes (Gilderhus and Marking, 1987;Hseu et al, 1998;Weber et al, 2009;Pawar et al, 2011). A Tukey's HSD test revealed that the induction time 500 μL LG 1 was significantly different from 400 μL LG 1 (193±14 sec, p = 0.004) and 600 μL LG 1 (190±14 sec, p = 0.347).…”

Section: Resultssupporting

confidence: 82%

“…Res., 11 (3): 102-106, 2015 stage was detected at 300 μL LG 1 . Longer recovery time with increased anaesthetic dosage has been reported in Hippocampus kuda Bleeker (Pawar et al, 2011) and Puntius denisonii (Day, 1865) (Sajan et al, 2012;Mercy et al, 2013). However, experiments by Mylonas et al (2005) documented recovery times decreasing with increase in anaesthetic concentration in Dicentrachus labrax and Sparus aurata (Linnaeus, 1758).…”

Section: Resultsmentioning

confidence: 88%

“…Experiments were repeated four times to verify the findings. The recovered fish were transferred into the observation tanks (1000 L) and held for 7 days, to assess the post recovery mortality (Pawar et al, 2011).…”

Section: Methodsmentioning

confidence: 99%

“…2-phenoxyethanol, a common fish anaesthetic is widely applied in sedation and transportation of fish (Guo et al, 1995). The effective anaesthetic concentration of 2-phenoxyethanol in a number of fish species have been reported to range between 200-600 µL LG 1 (Gilderhus and Marking, 1987;Guo et al, 1995;Weber et al, 2009;Pawar et al, 2011). Anaesthetising the fish is a practical option for facilitating ease of handling, improved egg yield and breeding response, besides significantly lowering brood mortality rates.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Adequacy Study of 2-Phenoxyethanol on Hypselobarbus kurali as an Anesthetic

Nai¹,

Williams²

2015

International J. of Zoological Research

View full text Add to dashboard Cite

Hypselobarbus kurali, one of the most demanded fresh water hill stream fish belonging to the family cyprinidae. In the case of brood H. kurali, injuries inflicted to the sudden death of the fish often pose a major problem. When large brood fish are involved, it can lead to incomplete voiding of eggs and mortality in most cases. Anaesthetizing the fish is a practical option for facilitating ease of handling, improved egg yield and breeding response, besides significantly lowering brood mortality rates. In this context, detailed studies were conducted on the use of a commonly employed anaesthetic 2-phenoxyethanol, in the handling and transportation of the fresh water hill stream fish Hypselobarbus kurali. Four levels of anaesthetic were tried viz., 300, 400, 500 and 600 µL LG 1 . The efficacy of the anaesthetic was assessed by considering four stages of induction (I 1 , I 2 , I 3 and I 4 ) and three stages of recovery (R 1 , R 2 and R 3 ). In the present study, the lowest induction time (<180 sec) was observed at 500 µL LG 1 and therefore, this dose was considered as the lowest effective concentration for anaesthesia in H. kurali. At 500 µL LG 1 , the time to reach anaesthesia stage (I3) of induction (159±13 sec) and recovery (R3) time (133±16 sec) was significantly different (p<0.05) from the other dosages 400 µL LG 1 ( 193±14 sec), 300 µL LG 1 ( 216±78 sec) and 600 µL LG 1 (190±14 sec).

show abstract

A fast and accurate isotope dilution GC‐IT‐MS/MS method for determination of eugenol in different tissues of fish: Application to a depletion study in mandarin fish

Liu

et al. 2018

Biomedical Chromatography

View full text Add to dashboard Cite

An accurate, rapid and effective method was established for determination of eugenol in plasma, muscle, skin, liver, kidney and gill of fish using gas chromatography-ion trap tandem mass spectrometry. Samples of muscle, skin, liver, kidney and gill were prepared using the modified QuEChERS (quick, easy, cheap, effective, rugged and safe) procedure, and a plasma sample was prepared by a liquid-liquid extraction procedure. Eugenol was monitored in <7 min using an electron-ionization source in MS/MS mode and quantified by an internal standard of eugenol-d . The limit of detection was 5.0 μg/kg, and the limit of quantification was 10.0 μg/kg. The calibration curve was linear in the range of 5-1000 μg/L (R = 0.9996). Intra- and inter-day precisions of eugenol expressed as relative standard deviation were within 9.74%, and the accuracy exhibited a relative error ranging from -2.20 to 8.89%. The developed method was successfully used to study the elimination regularity of eugenol in mandarin fish.

show abstract

Comparative efficacy of four anaesthetic agents in the yellow seahorse, Hippocampus kuda (Bleeker, 1852)

Cited by 60 publications

References 33 publications

The Efficacy of Clove Seed Extracts as an Anaesthetic Agent and Its Effect on Haematological Parameters of African Catfish (Clarias Gariepinus)

The Efficacy of Clove Seed Extracts as an Anaesthetic Agent and Its Effect on Haematological Parameters of African Catfish (Clarias Gariepinus)

Adequacy Study of 2-Phenoxyethanol on Hypselobarbus kurali as an Anesthetic

A fast and accurate isotope dilution GC‐IT‐MS/MS method for determination of eugenol in different tissues of fish: Application to a depletion study in mandarin fish

Contact Info

Product

Resources

About