Comparative efficacy of three extenders on quality of boar semen during preservation at 15°C

There is little information in the literature about the fungal contamination of boar semen and its persistence during storage. The challenge of this study was to perform a mycological screening to identify the yeast in the raw semen at 12/24 h after dilution. The research was done in pig farms in the N-E area of Romania, with maximum biosecurity and state-of-the-art technology. All the examined ejaculates (101) were considered to be normal for each spermogram parameter, with microbiological determinations in T0 at the time of ejaculate collection, T1 at the time of dilution, and T2 at 24 h of storage. Microbiological determinations (mycological spermogram) were performed for quantitative (LogCFU/mL) and qualitative (typification of fungal genera) identification. Bacterial burden (×103 LogCFU/mL) after dilution (T1) decreased drastically (p <0.0001) compared to the one in the raw semen (T0). After 24 h of storage at 17 °C, the mean value of the bacteriospermia remained constant at an average value of 0.44. Mycospermia had a constant trend at T0 (raw) and T1 (0.149 vs. 0.140) and was slightly higher at T2 (0.236). The difference between T1 vs. T2 (p = 0.0419) was close to the statistical reference value (p = 0.05). Of the total genera identified (24), the fungi had a proportion of 37.4% (9/15) and a ratio of 1:1.6. Regarding the total species (34), the fungi had a frequency of 29.42% (10/24) with a ratio between the fungi and bacteria of 1:2.4. A fertility rate of 86% was observed in the L1 group (50 AI sows with doses and mycospermia from T1), and an 82% rate was observed in the L2 group (50 AI sows with doses and mycospermia from T2). The litter size of L1 was 9.63 piglets and 9.56 for L2. Regarding the total number of piglets obtained between the two groups, there was a slight decrease of 22 piglets in group L2, without statistical differences (p > 0.05). The predominant genera persisted after dilution during a 12 h storage at 17 °C, where yeasts, such as Candida parapsilosis and C. sake were identified in more than 92% of AI doses.

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Candida Genus Maximum Incidence in Boar Semen Even after Preservation, Is It Not a Risk for AI though?

Ciornei

Drugociu

Roşca

2022

Molecules

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Effect of certain extenders on semen quality of boars during preservation at 17°C

KUMAR,

SINGH,

DHINDSA

et al. 2023

Indian J of Anim Sci

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The present study was designed to determine the effect of three selected extenders on liquid preservation of boar semen at 17°C. Ejaculates (30) were collected from three boars (one ejaculate/boar/week) housed at University pig farm, Ludhiana using dummy sow. Only ejaculates having >70% initial progressive motility were extended in Beltsville Thawing Solution (BTS), Safe Cell (SFC) and Tris-Egg Yolk (TEY) extenders in the ratio 1:4 and preserved in a BOD incubator at 17°C for 120 h. The semen was evaluated for different sperm attributes and lipid peroxidation (MDA) at 0, 24, 48, 72, 96 and 120 h of preservation. The mean percentage of sperm motility, viability,plasma membrane integrity and acrosome integrity were significantly higher in BTS as compared to SFC and TEY at different hours of storage period. At 120 h of preservation, highest percentage of in vitro capacitation/acrosome reaction was seen in semen stored in BTS followed by SFC and lowest in TEY after 6 h of incubation. Eventually, significantly lower levels of MDA were noticed in semen extended in BTS than in their contemporary extenders (SFC and TEY) at 96 and 120 h of storage period. The mean percentage of most sperm parameters decreased gradually from day of collection (0 h) up till 120 h in all the extenders. In conclusion, Beltsville Thawing Solution was better than Safe Cell and Tris-Egg Yolk extenders in liquid preservation of boar semen at 17oC owing to improved spermcharacteristics and reduced oxidative stress.

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Comparative efficacy of certain extenders on preservation of liquid boar semen

KAUR,

SINGH,

DHINDSA

et al. 2023

Indian J of Anim Sci

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This study was carried out to compare the efficacy of certain liquid semen extenders for boar semen preservation. Semen ejaculates (n=30) from three boars (one ejaculate/boar/week) stationed at University pig farm, Ludhiana were collected using dummy sow and extended (1:4) in lactose-sodium salt of EDTA-Egg yolk (LSEEY); glucose sodium salt of EDTA-potassium sodium tartrate-sodium citrate (GEPS); and Modena (MOD). The extended semen was kept at 17°C up to 120 h in a BOD incubator. The semen was assessed for various sperm characteristics and lipid peroxidation at 0, 24, 48, 72, 96 and 120 h of preservation. The results revealed significantly higher percentage of sperm motility, viability, plasma membrane integrity and acrosome integrity in semen extended with MOD thanin GEPS and LSEEY at different preservation times. The mean percentage of in vitro capacitation/acrosome reacted spermatozoa was significantly higher in MOD as compared to GEPS and LSEEY at 96 h and 120 h of storage period. In all the extenders, the mean percentage of most sperm attributes decreased progressively as the storage period increased. Correspondingly, the MDA levels were lower in MOD than in their contemporary extenders at all hours of preservation. In conclusion, Modena exhibited improved sperm parameters and reduced oxidative stress for liquid preservation of boar semen.

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Comparative efficacy of three extenders on quality of boar semen during preservation at 15°C

Cited by 3 publications

References 19 publications

Candida Genus Maximum Incidence in Boar Semen Even after Preservation, Is It Not a Risk for AI though?

Candida Genus Maximum Incidence in Boar Semen Even after Preservation, Is It Not a Risk for AI though?

Effect of certain extenders on semen quality of boars during preservation at 17°C

Comparative efficacy of certain extenders on preservation of liquid boar semen

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