Comparative embryotoxicity and proteotoxicity of three carrier solvents to zebrafish (Danio rerio) embryos

Hallare, Arnold V.; Nagel, Kerstin; Köhler, Heinz‐R.; Triebskorn, Rita

doi:10.1016/j.ecoenv.2005.07.006

Cited by 215 publications

(127 citation statements)

References 40 publications

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“…For over three decades it has been clear that ethanol exerts deleterious effects on the developing zebrafish embryo (e.g., Laale, 1971). Numerous recent studies have investigated the teratogenic and toxicological properties of this substance in zebrafish (Arenzana et al, 2006;Hallare et al, 2006) and zebrafish have also been proposed as a model of fetal alcohol syndrome (Bilotta et al, 2004). The behavioral effects of ethanol have also been studied in the zebrafish embryo (Lockwood et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Effects of acute and chronic ethanol exposure on the behavior of adult zebrafish (Danio rerio)

Gerlai

Lee

Blaser

2006

Pharmacology Biochemistry and Behavior

206

157

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The zebrafish has been a popular subject of embryology and genetic research for the past three decades. Recently, however, the interest in its neurobiology and behavior has also increased. Nevertheless, compared to other model organisms, e.g., rodents, zebrafish behavior is understudied and very few behavioral paradigms exist for mutation or drug screening purposes. Alcoholism is one of the biggest and costliest diseases whose mechanisms are not well understood. Model organisms such as the zebrafish may be utilized in this line of research. Previously, we investigated the effects of acute ethanol exposure on adult zebrafish using four behavioral paradigms and employing manual quantification methods. Here, we study the effects of chronic ethanol exposure and analyze how it modifies the effects of acute ethanol treatment. We employ a videotracking-based automated quantification method in a predator model paradigm and show that this method is capable of detecting an avoidance reaction that is ameliorated by higher doses of ethanol, a potential anxiolytic effect. Importantly, we also demonstrate that chronic, two week long, exposure to ethanol results in significant adaptation to this substance in adult zebrafish. Overall, our results suggest that zebrafish will be an appropriate subject for high throughput screening applications aimed at the analysis of the mechanisms and pharmacology of acute and chronic ethanol induced changes in the vertebrate brain.

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Section: Discussionmentioning

confidence: 99%

Effects of acute and chronic ethanol exposure on the behavior of adult zebrafish (Danio rerio)

Gerlai

Lee

Blaser

2006

Pharmacology Biochemistry and Behavior

206

157

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“…13 For example, the lowest effective dose that causes gross morphological defects after exposure for 96 hpf to TCDD and ethanol is 0.005 lM and 1% (218 mM), respectively; and after exposure for 48 hpf to TCCD and 24 hpf to ethanol is 0.1 lM and 200 mM, respectively. 21,24,27 Overall, nitrite caused developmental defects in zebrafish at concentrations close to those for ethanol, a known human No defects were observed at 250, 500, 750, and 1000 mg/L nitrite.…”

Section: Discussionmentioning

confidence: 99%

Effects of Nitrite on Development of Embryos and Early Larval Stages of the Zebrafish (Danio rerio)

Simmons¹,

Karimi²,

Talwar

et al. 2012

Zebrafish

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Epidemiological studies suggest that high nitrate levels in food and water may cause birth defects or spontaneous abortions in humans. Experimental mammalian studies show that high nitrite levels adversely affect reproductive outcomes, but have not shown congenital malformations. Consequently, the teratogenic potential of nitrite is unclear. In this study, the effects of nitrite on development of zebrafish embryos and early larval stages were investigated. Eggs were exposed to ethanol (a known teratogen), nitrite, or nitrate for 24 or 96 hours, and larvae examined at 120 hours. Sublethal exposure to 300 mM ethanol for 24 hours caused severe pericardial and yolk sac edema, craniofacial and axial malformations, and swim bladder noninflation. The 96 hour LC 50 for nitrite was 411 mg/L. Less severe edema, craniofacial (but not axial) malformations, swim bladder noninflation, and immobility were observed after sublethal exposure to nitrite between 10 and 300 mg/L for 96 hours. Exposure to nitrite for 24 hours at concentrations as high as 2000 mg/L was not lethal. Only axial malformations and swim bladder noninflation were observed at 1500 mg/L. The results demonstrate that sublethal nitrite concentrations cause developmental defects. The type and magnitude of these defects differed after 24 and 96 hours of exposure.

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“…Although not significantly different, a 12% higher mean weight of fish exposed to the lowest concentration (0.2 ml·l -1 ) of DMSO, compared to the control group was found in the current study. Hallare et al (2006) tested the effect of 48 and 96-h exposure of DMSO (concentration of 0.0001% DMSO (v/v) to 2.0% DMSO (v/v), e.g. 0.001 ml·l -1 to 20 ml·l -1 ) on early life stage indicators, such as egg and embryo mortality, gastrulation, somite formation, movement and tail detachment, pigmentation, heart beat, and hatching success and the levels of heat shock proteins (hsp 70) of zebrafish.…”

Section: Discussionmentioning

confidence: 99%

“…A major concern in toxicology studies is the choice of appropriate solvents. Carrier solvents may also have toxic effects on organisms (Hallare et al 2006;Ball 1966;Rayburn and Fisher 1997;Benville 1968). Hence, it was necessary to set guidelines that will describe the types and the maximum allowable concentrations of the solvent to be used in experimental systems that will cause no significant impact on the observed results (Hallare et al 2006).…”

Section: Dmso Auxiliary Substance Toxicity Fish Embryos and Larvaementioning

confidence: 99%

“…Carrier solvents may also have toxic effects on organisms (Hallare et al 2006;Ball 1966;Rayburn and Fisher 1997;Benville 1968). Hence, it was necessary to set guidelines that will describe the types and the maximum allowable concentrations of the solvent to be used in experimental systems that will cause no significant impact on the observed results (Hallare et al 2006).Dimethyl sulphoxide (DMSO) is commonly used in fish toxicity tests as a solvent for several water-insoluble substances (Rufli et al 1998;Rayburn et al 1991). DMSO is a colourless hygroscopic liquid that is synthesized by the oxidation of dimethyl sulphide, or originates as a waste product from wood pulp processing.…”

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confidence: 99%

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Early Ontogeny, Growth and Mortality of Common Carp (Cyprinus carpio) at Low Concentrations of Dimethyl Sulfoxide

et al. 2009

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Dimethyl sulphoxide (DMSO) is an important polar aprotic solvent, less toxic than other members of this class. Because of its excellent solvating power, DMSO is frequently used as solvent for chemical reactions involving salts, especially Finkelstein reactions and other nucleophilic substitutions. Furthermore, DMSO is used as auxiliary substance in toxicity tests on aquatic organisms, usually at concentrations of 0.2 and 0.5 ml·l -1 . The aim of the present study was to evaluate the effect of DMSO on early development, growth and mortality of early life stages in common carp. Tests were performed from fertilized egg to the end of the larval period with continuous exposure to DMSO at concentrations of 0.2, 0.5, 1.0 and 5.0 ml·l -1 . Mortality of embryos and larvae, intensity of development and growth, weight and occurrence of abnormalities were analyzed. DMSO exposure at all the tested concentrations did not result in any lethal effects or abnormalities compared to the control over the entire test period (29 days). Growth indicators were similar in DMSO treated (at concentrations of 0.2, 0.5 and 1.0 ml·l ) and control groups. However, mean body weight (35 ± 9 mg) of larvae exposed to the highest DMSO concentration (5 ml·l -1 ) tested was significantly lower compared to the control (45 ± 14 mg). DMSO, auxiliary substance, toxicity, fish embryos and larvaeAquatic toxicology tests are designed for readily water soluble substances. However, due to the occurrence of several water-insoluble substances, the use of various solvents is needed. A major concern in toxicology studies is the choice of appropriate solvents. Carrier solvents may also have toxic effects on organisms (Hallare et al. 2006;Ball 1966;Rayburn and Fisher 1997;Benville 1968). Hence, it was necessary to set guidelines that will describe the types and the maximum allowable concentrations of the solvent to be used in experimental systems that will cause no significant impact on the observed results (Hallare et al. 2006).Dimethyl sulphoxide (DMSO) is commonly used in fish toxicity tests as a solvent for several water-insoluble substances (Rufli et al. 1998;Rayburn et al. 1991). DMSO is a colourless hygroscopic liquid that is synthesized by the oxidation of dimethyl sulphide, or originates as a waste product from wood pulp processing. Its chemical structure creates extraordinary properties. The sulphur-oxygen bond determines the polar nature of DMSO with the capability to dissolve sparingly soluble substances, and it is completely miscible in water, common organic solvents and unsaturated hydrocarbons (Willhite and Katz 1984). Furthermore, the chemical structure of DMSO enables it to penetrate membrane barriers by increasing the permeability without affecting the morphologic characteristics of the skin and body membranes. Therefore, DMSO acts as a skin penetration enhancer for drugs and other substances (Kligman 1965a; Kligman 1965b;Jacob et al. 1964;Stoughton and Fritsch 1964). The main applications and properties of DMSO were summarized in reviews...

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Comparative embryotoxicity and proteotoxicity of three carrier solvents to zebrafish (Danio rerio) embryos

Cited by 215 publications

References 40 publications

Effects of acute and chronic ethanol exposure on the behavior of adult zebrafish (Danio rerio)

Effects of acute and chronic ethanol exposure on the behavior of adult zebrafish (Danio rerio)

Effects of Nitrite on Development of Embryos and Early Larval Stages of the Zebrafish (Danio rerio)

Early Ontogeny, Growth and Mortality of Common Carp (Cyprinus carpio) at Low Concentrations of Dimethyl Sulfoxide

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