Comparative erythrocyte deformability investigations by filtrometry, slit-flow and rotational ektacytometry in a long-term follow-up animal study on splenectomy and different spleen preserving operative techniques: Partial or subtotal spleen resection and spleen autotransplantation

Miko, Iren; Németh, Norbert; Sógor, Viktória; Kiss, Ferenc; Tóth, Enikő; Pető, Károly; Furka, Andrea; Vanyolos, Erzsebet; Tóth, L; Varga, József; Szigeti, Krisztián; Benkö, Ilona; Oláh, Anna V.; Furka, I.

doi:10.3233/ch-160231

Cited by 6 publications

(3 citation statements)

References 21 publications

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“…1). Furthermore, when the effectiveness of eliminating red blood cells with impaired deformability worsens, the rheology of circulating blood can be altered that, for instance, happens after splenectomy or in cases of hyposplenic/asplenic conditions [65].…”

Section: Main Factors and Pathophysiological Processes Affecting The Rheology Of Bloodmentioning

confidence: 99%

Effects and influencing factors on hemorheological variables taken into consideration in surgical pathophysiology research

Németh

Deák

Szentkereszty

et al. 2018

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In surgical pathophysiology ischemia-reperfusion, inflammatory processes, sepsis, vascular interventions, tissue trauma, shock, all mean conditions in which hemorheological parameters show alterations. Despite of numerous clinical and experimental studies, the in vivo hemorheology is not completely understood yet, and several fundamental questions still need to be answered. Investigating these issues, experimental surgical models are important, in point of view of the translational research as well. In this paper we aimed to make an attempt on summarizing the possible factors and conditions that might have an effect on hemorheological results in experimental surgical studies. Hemorheological parameters show alterations in surgical pathophysiological processes in a complex way. However, the changes are dominantly non-specific. Standardized experimental conditions, related to the experimental animal (species, animal welfare) anesthesia-medications, operation, sampling and, if applicable, conditions of the postoperative period, are inevitable for a safe assessment of valuable (hemorheological) results. Parallel investigations - such as microcirculatory monitoring, imaging techniques, other laboratory methods, histomorphology- have great importance, together with individual analysis of changes, for a better understanding of the changes and for comparability with clinical results.

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Section: Main Factors and Pathophysiological Processes Affecting The Rheology Of Bloodmentioning

confidence: 99%

Effects and influencing factors on hemorheological variables taken into consideration in surgical pathophysiology research

Németh

Deák

Szentkereszty

et al. 2018

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“…It is clear that the most important regulator of erythrocyte deformability is the clearance of rigid erythrocytes within the spleen. A mechanical checking of the deformability of circulating erythrocytes is regularly performed in the splenic microcirculation, so that the RBCs that are not able to correctly squeeze through the narrow splenic slits are trapped and removed from circulation [25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Metabolic Influences Modulating Erythrocyte Deformability and Eryptosis

et al. 2021

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Many factors in the surrounding environment have been reported to influence erythrocyte deformability. It is likely that some influences represent reversible changes in erythrocyte rigidity that may be involved in physiological regulation, while others represent the early stages of eryptosis, i.e., the red cell self-programmed death. For example, erythrocyte rigidification during exercise is probably a reversible physiological mechanism, while the alterations of red blood cells (RBCs) observed in pathological conditions (inflammation, type 2 diabetes, and sickle-cell disease) are more likely to lead to eryptosis. The splenic clearance of rigid erythrocytes is the major regulator of RBC deformability. The physicochemical characteristics of the surrounding environment (thermal injury, pH, osmolality, oxidative stress, and plasma protein profile) also play a major role. However, there are many other factors that influence RBC deformability and eryptosis. In this comprehensive review, we discuss the various elements and circulating molecules that might influence RBCs and modify their deformability: purinergic signaling, gasotransmitters such as nitric oxide (NO), divalent cations (magnesium, zinc, and Fe++), lactate, ketone bodies, blood lipids, and several circulating hormones. Meal composition (caloric and carbohydrate intake) also modifies RBC deformability. Therefore, RBC deformability appears to be under the influence of many factors. This suggests that several homeostatic regulatory loops adapt the red cell rigidity to the physiological conditions in order to cope with the need for oxygen or fuel delivery to tissues. Furthermore, many conditions appear to irreversibly damage red cells, resulting in their destruction and removal from the blood. These two categories of modifications to erythrocyte deformability should thus be differentiated.

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“…While this can be interpreted as fair survival data, a proportion of dogs (7.6%) will develop complications secondary to splenectomy because of thrombotic or coagulopathic syndromes [9]. Additional adverse effects following splenectomy in dogs have included peri- and post-operative ventricular arrhythmias [9–11], reduced blood filtration and renewal [12,13], impairment of humoral immune response [14], reduced immune-surveillance against bacteria and parasites [15–19], and higher incidence of gastric dilatation-volvulus [6,20–22]. For these reasons, any preoperative diagnostic approach to splenic lesions, including cytology, may be beneficial in preventing unnecessary splenectomy.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of cytological diagnostic accuracy for canine splenic neoplasms: An investigation in 78 cases using STARD guidelines

et al. 2019

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Cytology represents a useful diagnostic tool in the preliminary clinical approach to canine splenic lesions, and may prevent unnecessary splenectomy. However, few studies have evaluated diagnostic accuracy of cytology in the diagnosis of canine splenic neoplasms. The aim of this study was to determine overall accuracy, sensitivity, specificity, positive and negative predictive values (i.e. diagnostic accuracy indexes) of cytology for canine splenic neoplasms following Standards for the Reporting of Diagnostic Accuracy Studies (STARD) guidelines. A consecutive series of canine splenic cytological samples was retrospectively retrieved from the database of the Diagnostic Pathology Service of the Department of Veterinary Medicine (DIMEVET—University of Milan). Histopathology was set as the diagnostic reference standard. Cytological cases were enrolled when slides were available for review and when the same lesion was submitted for histopathology. Seventy-eight (78) lesions were included in the study. By histopathology, 56 were neoplastic and 22 were non-neoplastic. Cytology had an overall accuracy of 73.08% (95% C.I. 61.84%-82.50%), sensitivity of 64.29% (95% C.I. 50.36%-76.64%), specificity of 95.45% (95% C.I. 77.16%-99.88%), and positive and negative predictive values of 97.3% (95% C.I. 84.01%-99.60%) and 51.22% (95% C.I. 42.21%-60.15%), respectively. Low sensitivity and negative predictive value were balanced by very high specificity and positive predictive value. When positive for neoplasia, cytology represents a useful diagnostic tool to rule in splenic neoplasia, prompting surgery independently from other diagnostic tests. Conversely, a negative cytological result requires additional investigations to confirm the dog to be disease free.

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Comparative erythrocyte deformability investigations by filtrometry, slit-flow and rotational ektacytometry in a long-term follow-up animal study on splenectomy and different spleen preserving operative techniques: Partial or subtotal spleen resection and spleen autotransplantation

Cited by 6 publications

References 21 publications

Effects and influencing factors on hemorheological variables taken into consideration in surgical pathophysiology research

Effects and influencing factors on hemorheological variables taken into consideration in surgical pathophysiology research

Metabolic Influences Modulating Erythrocyte Deformability and Eryptosis

Evaluation of cytological diagnostic accuracy for canine splenic neoplasms: An investigation in 78 cases using STARD guidelines

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