Comparative evaluation of antibacterial activity of total-etch and self-etch adhesive systems: An ex vitro study

Abstract

Aim:The aim of this ex vivo study was to compare the antibacterial activity of total-etch and self-etch adhesive systems against Streptococcus mutans, Lactobacillus acidophilus, and Actinomyces viscosus through disk diffusion method.Materials and Methods:The antibacterial effects of Single Bond (SB) and Adper Prompt (AP) and aqueous solution of chlorhexidine 0.2% (positive control) were tested against standard strain of S. mutans, L. acidophilus, and A. viscosus using the disk diffusion method. The diameters of inhibition zones were measured in millimeters. Data was analyzed using Kruskal-Wallis test. Mann-Whitney U test was used for pairwise comparison.Result:Of all the materials tested, AP showed the maximum inhibitory action against S. mutans and L. acidophilus. Aqueous solution of chlorhexidine 0.2% showed the maximum inhibitory action against A. viscosus. Very minimal antibacterial effect was noted for SB.Conclusion:The antibacterial effects observed for the tested different dentin bonding systems may be related to the acidic nature of the materials.

Drug loaded homogeneous electrospun PCL/gelatin hybrid nanofiber structures for anti-infective tissue regeneration membranes

“…The antibacterial activity of the membranes against the typical anaerobic bacterium Fusobacterium nucleatum (ATCC 25586, Chinese General Microbiological Culture Collection Center), which is commonly found in the oral cavity in an infection [28], was determined by the modified Kirby-Bauer method [29] under anaerobic conditions [30]. Anaerobic environment was created by using MGC AnaeroPack TM -Anaero Series (Anaero-2.5 L is a disposable oxygen-absorbing and carbon dioxide generating agent for use in anaerobic jars, which has been widely used in laboratories to create anaerobic environment).…”

“…Acetic acid (HAc) was introduced to improve the miscibility of PCL and gelatin to fabricate homogeneous hybrid nanofiber membranes. The effects of the addition of HAc and the MNA content (0,1,5,10,20,30, and 40 wt.% of polymer) on the properties of the membranes were investigated. The membranes showed good mechanical properties, appropriate biodegradation rate and barrier function.…”

Chitosan in different concentrations added to a two-step etch-and-rinse adhesive system: influence on bond strength to dentin

“…At the time of incorporating chitosan, or other materials into the adhesive system, the pH must be acid, because chitosan is insoluble at pH values higher than 7.0 (basic mean) and extremely soluble in an acid medium. Adper Single Bond 2 and the experimental adhesive system present a pH around 4.5 [21,30], which facilitate the incorporation of chitosan. The study of Elsaka [21] tested the bond strength and pH of adhesives containing different concentrations of chitosan (0.12%, 0.25%, 0.5% and 1%), observing that the lower the pH, the higher the concentration of chitosan, and that there was a reduction in bond strength with the increase in the concentration of the product.…”

The Effect of Bioactive Chitosan Nanoparticles on Micro-Shear Bond Strength to Dentin (an in vitro study)

“…The monomer/solvent combination in adhesive systems with their application performed in a larger number of steps must be capable of breaking the interpeptide hydrogen bridges, and thus re-expand the dentin matrix, recreating the interfibrillar spaces of the collapsed collagen network to allow infiltration of the hydrophobic monomer (25) .…”