Comparative evaluation of antibiofilm efficacy of chitosan nanoparticle- and zinc oxide nanoparticle-incorporated calcium hydroxide-based sealer: An In vitro study

Nair, Narayan; James, Baby; Johny, Minimol K; Mathew, Josey; Jacob, José Luiz Balthazar

doi:10.4103/ccd.ccd_225_18

Cited by 25 publications

(9 citation statements)

References 29 publications

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“…The second mechanism is the production of free radicals such as reactive oxygen species which can affect the resistance of bacterial cells by inhibiting protein function and damaging deoxyribonucleic acid. 22 This present study revealed the development of the inhibition zone of ERB sealer also occurred with no addition of chitosan nanoparticles. It might be due to the presence of antibacterial components, such as hexamethylenetetramine and formaldehyde in the ERB sealer used in this study (AH 26).…”

Section: Discussionsupporting

confidence: 56%

Antibacterial and Cytotoxicity of Root Canal Sealer with the Addition of Chitosan Nanoparticle at Various Concentrations

et al. 2022

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Objectives The aim of this study was to evaluate the addition of chitosan nanoparticle with concentrations of 0, 10, 20, and 30% to the epoxy resin-based (ERB) sealer on its antibacterial and cytotoxicity effect. Methods and Materials This research was divided into two studies, the first study was the addition of chitosan with a concentration of 0% (as control), 10, 20, and 30% to an ERB sealer on its antibacterial effect, and the second study was on its cytotoxicity. An agar diffusion test was employed to determine the antibacterial effect on Enterococcus faecalis. An MTT (3-{4,5-dimethylthiazol-2-yl}-2,5-diphenyl tetrazolium bromide) assay was utilized to test the cytotoxicity by evaluating cell viability. Statistical Analysis One-way analysis of variance and Tukey's test (α = 0.05) were used to analyze data obtained from each evaluation with a significance level of 95%. Results The addition of chitosan nanoparticles at concentrations 10, 20, and 30% produced a greater inhibition zone of E. faecalis (p < 0.05), however, had less cytotoxicity compared with no addition of chitosan (0%) (p < 0.05). Conclusion The addition of chitosan nanoparticles at concentrations 10, 20, and 30% to the ERB sealer produced greater antibacterial and less cytotoxicity compared with no addition of chitosan (0%).

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Section: Discussionsupporting

confidence: 56%

Antibacterial and Cytotoxicity of Root Canal Sealer with the Addition of Chitosan Nanoparticle at Various Concentrations

et al. 2022

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“…Likewise, Nair et al tested the antibiofilm potential of ZnO‐NPs and chitosan NPs against Enterococcus faecalis (OG1RF) and Enterococcus faecalis (ATCC 29212). Chitosan NPs treatment significantly reduced the growth of E.faecalis (ATCC 29212) biofilm and Zno‐NPs found to exhibit antibiofilm properties against both of the strains 40 …”

Section: Resultsmentioning

confidence: 99%

“…Chitosan NPs treatment significantly reduced the growth of E.faecalis (ATCC 29212) biofilm and Zno-NPs found to exhibit antibiofilm properties against both of the strains. 40 Recently, Khan et al 41 claimed that the sol-gel fabricated Zn nanospikes exhibits antibiofilm activity against Pseudomonas aeruginosa. However, ZN1 nanospikes reduced biofilm formation around (23-58%) at 200 μg/mL concentration and ZN2 nanospikes reduced maximum level of (85%) at 200 μg/mL concentration.…”

Section: Antibiofilm Activitymentioning

confidence: 99%

Characterization and therapeutic potential of chitosan‐zinc oxide nanostructured particles synthesized using crab shell derived biopolymer

Rajan,

Saravanan,

Mohan

et al. 2024

Polymers for Advanced Techs

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The development of novel drug candidates for treating microbial infections and cancer turns into a challenging task. The biopolymer‐based nanomaterials have gained popularity for their effective roles in the pharmaceutical sector. The present investigation aims to valorize crab shell wastes to extract chitosan. The obtained (chitosan) biomass was utilized to synthesize chitosan‐zinc oxide nanoparticles (Ch‐ZnO‐NPs). The nanomaterial characterization was achieved by a variety of physicochemical techniques including UV, Fourier transform infrared spectroscopy (FT‐IR), X‐ray diffraction (XRD), elemental analysis, and High‐resolution transmission electron microscopic (HR‐TEM) examination. The synthesized nanomaterials have a particle size range of 35–80 nm and are more stable with a zeta potential value of (−40.78) mv. Additionally, the designed nanomaterials were utilized to evaluate the biological efficiency. The minimal inhibitory concentration value of Ch‐ZnO‐NPs was observed as 10 μg/mL. The designed nanomaterials significantly reduced the growth of Vibrio alginolyticus and Bacillus cereus and inhibited biofilm formation. The synthesized Ch‐ZnO‐NPs unveiled cytotoxic effects on A549 cells with an IC50 (half‐maximal inhibitory concentration) of 20 μg/mL. Furthermore, substantial increase in cell death was noticed in A549 cells treated with Ch‐ZnO‐NPs. Consequently, this investigation concludes that Ch‐ZnO‐NPs may act as a biomedicine.

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“…Data from bacteriostasis circle and SEM micrographs have identified that the synergistic bactericidal activity of chitosan NPs and CHX had the strongest inhibition against E. faecalis , irrespective of the types of sealers. However, one study by Nair et al [ 159 ] showed that the incorporation of chitosan NPs in Ca(OH) 2 sealer (i.e., Apexit Plus) failed to improve its antibacterial properties against E. faecalis strain OG1RF. Moreover, Ratih et al [ 160 ] found that the increment of chitosan NP concentration from 10% to 30% in epoxy resin-based sealer (AH-26) did not significantly enlarge E. faecalis inhibition zone, indicating the need for more comprehensive investigations to optimize the antimicrobial effects of chitosan NPs.…”

Section: Nanomaterials As Endodontic Sealersmentioning

confidence: 99%

Application of Nanomaterials in Endodontics

Afkhami,

Chen,

Walsh

et al. 2024

BME Front

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Recent advancements in nanotechnology have introduced a myriad of potential applications in dentistry, with nanomaterials playing an increasing role in endodontics. These nanomaterials exhibit distinctive mechanical and chemical properties, rendering them suitable for various dental applications in endodontics, including obturating materials, sealers, retro-filling agents, and root-repair materials. Certain nanomaterials demonstrate versatile functionalities in endodontics, such as antimicrobial properties that bolster the eradication of bacteria within root canals during endodontic procedures. Moreover, they offer promise in drug delivery, facilitating targeted and controlled release of therapeutic agents to enhance tissue regeneration and repair, which can be used for endodontic tissue repair or regeneration. This review outlines the diverse applications of nanomaterials in endodontics, encompassing endodontic medicaments, irrigants, obturating materials, sealers, retro-filling agents, root-repair materials, as well as pulpal repair and regeneration. The integration of nanomaterials into endodontics stands poised to revolutionize treatment methodologies, presenting substantial potential advancements in the field. Our review aims to provide guidance for the effective translation of nanotechnologies into endodontic practice, serving as an invaluable resource for researchers, clinicians, and professionals in the fields of materials science and dentistry.

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Comparative evaluation of antibiofilm efficacy of chitosan nanoparticle- and zinc oxide nanoparticle-incorporated calcium hydroxide-based sealer: An In vitro study

Cited by 25 publications

References 29 publications

Antibacterial and Cytotoxicity of Root Canal Sealer with the Addition of Chitosan Nanoparticle at Various Concentrations

Antibacterial and Cytotoxicity of Root Canal Sealer with the Addition of Chitosan Nanoparticle at Various Concentrations

Characterization and therapeutic potential of chitosan‐zinc oxide nanostructured particles synthesized using crab shell derived biopolymer

Application of Nanomaterials in Endodontics

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